Protein Isoelectric Point

Sequence Manipulation Suite:

Protein Isoelectric Point

Protein Isoelectric Point calculates the theoretical pI (isoelectric point) for the protein sequence you enter. Use Protein Isoelectric Point when you want to know approximately where on a 2-D gel a particular protein will be found.

Paste the raw sequence or one or more FASTA sequences into the text area below. Input limit is 200,000,000 characters.
>sequence 1 GAMPSTRV >sequence 2 MPSTYLLQ

• Add 1 2 3 4 5 copies of Nothing AU1 (DTYRYI) AU5 (TDFYLK) BTag (QYPALT) c-myc (EQKLISEEDL) FLAG (DYKDDDDK) GFP (MSKGEELFTG...) Glu-Glu (EYMPME) Glu-Glu (EFMPME) GST (MSPILGYWKI...) HA (YPYDVPDYA) His6 (HHHHHH) HSV (QPELAPEDPE...) HTTPHH (HTTPHH) IRS (RYIRS) KT3 (PPEPET) Protein C (EDQVDPRLID...) S-Tag (KETAAAKFER...) SBP (MDEKTTGWRG...) T7 (MASMTGGQQM...) V5 (GKPIPNPLLG...) VSV-G (MNRLGK) to each sequence.
• Use pK values from EMBOSS DTASelect

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Sun 1 Oct 12:00:07 2023

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