第四室(ワクチン・血液室)
当室では、業務として血液製剤 (アルブミン製剤、ハプトグロビン製剤)の製剤担当室として、血液製剤の安全性向上に関する基礎研究を行っています。また生物学的製剤(ワクチン及び血液製剤)の安全性に関わる国家検定・検査 (発熱試験・エンドトキシン試験(準備中)) を行っています。また、HTLV-1感染の効果的検査法の開発、国家検定の安全評価法の開発、細菌性ベクター及び粘膜アジュバンドを用いた新規予防・治療法の開発、既存の安全性試験法の改良及び新しい安全性試験法の研究・開発を行っています。HTLV-1感染の効果的検査法の開発や成人T細胞白血病 (ATL) の基礎的研究を行っております。
メンバー
室長
倉 光 球
主任研究官
谷 生 道 一
前 山 順 一
非常勤職員
石 井 美 枝 子
業務内容
1. 血液製剤(人血清アルブミン、人ハプトグロビン)の国家検定・検査
2. 品質管理に関する業務、研究
発熱試験、エンドトキシン試験、承認前試験
研究内容
1. HTLV-1感染の効果的検査法の開発
2. 国家検定の安全評価法の開発
3. 細菌性ベクター及び粘膜アジュバンドを用いた新規予防・治療法の開発
研究業績
【原著論文(英文)】
- Recombinant mycobacterial DNA-binding protein 1 with post-translational modifications boosts IFN-gamma production from BCG-vaccinated individuals' blood cells in combination with CpG-DNA.
Ozeki Y, Yokoyama A, Nishiyama A, Yoshida Y, Ohara Y, Mashima T, Tomiyama C, Shaban AK, Takeishi A, Osada-Oka M, Yamaguchi T, Tateishi Y, Maeyama JI, Hakamata M, Moro H, Kikuchi T, Hayashi D, Suzuki F, Yamamoto T, Iho S, Katahira M, Yamamoto S, Matsumoto S.
Sci Rep, 14:9141, 2024 - Establishment of a Novel Human T-cell Leukemia Virus Type 1 Infection Model Using Cell-Free Virus.
Nagata K, Tezuka K, Kuramitsu M, Fuchi N, Hasegawa Y, Hamaguchi I, Miura K.
J Virol, 98:e0186223, 2024 - Development of a novel multiplex digital PCR-based method for the detection of HTLV-1 proviral deletion.
Hiraga K, Tezuka K, Nagata K, Koh KR, Nakamura H, Sagara Y, Sobata R, Satake M, Tanio M, Hasegawa H, Saito M, Miura K, Mizukami T, Hamaguchi I, Kuramitsu M.
J Virol Methods, 332:115071, 2024 - Highly homologous simian T-cell leukemia virus type 1 genome in Japanese macaques: a large cohort study.
Hiraga K, Kitamura T, Kuramitsu M, Murata M, Tezuka K, Okuma K, Hamaguchi I, Akari H, Mizukami T.
Virol J, 21:166, 2024 - Emergence of BS.1 and BS.1.1, highly mutated new SARS-CoV-2 Omicron variants.
Takahashi K, Saito M, Sekizuka T, Itokawa K, Tanio M, Yoshida-Furihata H, Nojiri N, Hamada Y, Yokota E, Kuroda M, Saito T.
J Med Virol, 95:e28615, 2023 - Effect of cigarette smoke on mucosal vaccine response with activation of plasmacytoid dendritic cells: The outcomes of in vivo and in vitro experiments.
Suzuki F, Maeyama JI, Kubota A, Nishimune A, Horiguchi S, Takii T, Urasaki Y, Shimada I, Iho S.
Vaccine, 41:1447-1456, 2023 - Report on the seventh meeting of national control laboratories for vaccines and biologicals of the WHO Western Pacific and South-East Asia member states.
Shim SB, Choi CW, Shin JH, Kim JW, Schepelmann S, Jung JH, Chander H, Pujilestari R, Kuramitsu M, Ochiai M, Qi NY, Dimapilis GN, Dung LT, Moon HS, Shin IS.
Biologicals, 84:101712, 2023 - Efficacy of convalescent plasma therapy for COVID-19 in Japan: An open-label, randomized, controlled trial.
Saito S, Kutsuna S, Akifumi I, Hase R, Oda R, Terada J, Shimizu Y, Uemura Y, Takamatsu Y, Yasuhara A, Shiratori K, Satake M, Sakamoto N, Miyazaki Y, Shimizu H, Togano T, Matsunaga A, Okuma K, Hamaguchi I, Fujisawa K, Nagashima M, Ashida S, Terada M, Kimura A, Morioka S, Matsubayashi K, Tsuno NH, Kojima M, Kuramitsu M, Tezuka K, Ikebe E, Ishizaka Y, Kenji M, Hangaishi A, Mikami A, Sugiura W, Ohmagari N, Mitsuya H.
J Infect Chemother, 29:869-874, 2023 - Performance evaluation of in vitro diagnostic kits for hepatitis B virus infection using the regional reference panel of Japan.
Momose H, Murayama A, Yamada N, Matsubayashi K, Matsuoka S, Ikebe E, Kuramitsu M, Muramatsu M, Kato T, Hamaguchi I.
Virol J, 20:93, 2023 - Performance evaluation of Espline HTLV-I/II, a newly developed rapid immunochromatographic antibody test for different diagnostic situations.
Kuramitsu M, Momose H, Uchida Y, Ishitsuka K, Kubota R, Tokunaga M, Utsunomiya A, Umekita K, Hashikura Y, Nosaka K, Koh KR, Nakamura H, Sagara Y, Sobata R, Satake M, Nagata K, Hasegawa Y, Sasaki D, Hasegawa H, Sato T, Yamano Y, Hiraga K, Tezuka K, Ikebe E, Matsuoka S, Okuma K, Watanabe T, Miura K, Hamaguchi I.
Microbiol Spectr, 11:e0207823, 2023 - Vaccination with short-term-cultured autologous PBMCs efficiently activated STLV-1-specific CTLs in naturally STLV-1-infected Japanese monkeys with impaired CTL responses.
Hasegawa A, Murata M, Fujikawa T, Katagiri K, Nagano Y, Masuda T, Kuramitsu M, Nakajima S, Fujisawa JI, Okuma K, Grover P, Kidiga M, Akari H, Kannagi M.
PLoS Pathog, 19:e1011104, 2023 - RAISING is a high-performance method for identifying random transgene integration sites.
Wada Y, Sato T, Hasegawa H, Matsudaira T, Nao N, Coler-Reilly ALG, Tasaka T, Yamauchi S, Okagawa T, Momose H, Tanio M, Kuramitsu M, Sasaki D, Matsumoto N, Yagishita N, Yamauchi J, Araya N, Tanabe K, Yamagishi M, Nakashima M, Nakahata S, Iha H, Ogata M, Muramatsu M, Imaizumi Y, Uchimaru K, Miyazaki Y, Konnai S, Yanagihara K, Morishita K, Watanabe T, Yamano Y, Saito M.
Commun Biol, 5:535, 2022 - Calcium-dependent reversible coaggregation activity of C-reactive protein and M-ficolin.
Tanio M.
Mol Immunol, 149:157-164, 2022 - Comparative Study of the Susceptibility to Oxidative Stress between Two Types of Mycobacterium bovis BCG Tokyo 172.
Taniguchi K, Hayashi D, Yasuda N, Nakayama M, Yazawa K, Ogawa S, Miyatake Y, Suda S, Tomita H, Tokuda M, Itoh S, Maeyama JI, Ohara N, Yamamoto S, Hida S, Onozaki K, Takii T.
mSphere, 6, 2021 - No SARS-CoV-2 RNA detected in the convalescent plasma of COVID-19 patients with different disease severity.
Nomoto H, Kutsuna S, Okuma K, Kuramitsu M, Tezuka K, Ikebe E, Saito S, Kinoshita N, Terada M, Endo M, Suzuki T, Miyazato Y, Nakamoto T, Inada M, Hamaguchi I, Ohmagari N.
J Infect Chemother, 27:653-655, 2021 - Evaluation of a booster tuberculosis vaccine containing mycobacterial DNA-binding protein 1 and CpG oligodeoxynucleotide G9.1 using a Guinea pig model that elicits immunity to Bacillus Calmette-Guerin.
Maeyama JI, Iho S, Suzuki F, Hayashi D, Yamamoto T, Yamazaki T, Goto Y, Ozeki Y, Matsumoto S, Yamamoto S.
Tuberculosis (Edinb), 128:102067, 2021 - First case of molecularly identified and genetically characterized human T-cell leukemia virus type 2 infection in a pregnant woman in non-endemic Japan.
Kuramitsu M, Okuma K, Horiya M, Sekizuka T, Kaneko N, Saito E, Sokunaga J, Kuroda M, Hamaguchi I.
J Virol Methods, 287:114005, 2021 - Streptococcus pyogenes TrxSR Two-Component System Regulates Biofilm Production in Acidic Environments.
Isaka M, Okamoto A, Miura Y, Tatsuno I, Maeyama JI, Hasegawa T.
Infect Immun, 89:e0036021, 2021 - Validation of HPLC Method for Determination of Histamine in Human Immunoglobulin Formulations.
Tanio M, Nakamura T, Kusunoki H, Ideguchi K, Nakashima K, Hamaguchi I.
Journal of AOAC International, 103, 1223–1229, 2020 - A high-throughput detection method for the clonality of Human T-cell leukemia virus type-1-infected cells in vivo.
Saito M, Hasegawa H, Yamauchi S, Nakagawa S, Sasaki D, Nao N, Tanio M, Wada Y, Matsudaira T, Momose H, Kuramitsu M, Yamagishi M, Nakashima M, Nakahata S, Iha H, Ogata M, Imaizumi Y, Uchimaru K, Morishita K, Watanabe T, Miyazaki Y, Yanagihara K.
International journal of hematology, 112, 300–306, 2020
【著書・総説,その他(和文)】
- 倉光球「HTLV-1感染を捉える検査の進展」日本臨床検査医学会誌、2024年、72(5)、416-418.
【その他】
- 「HTLV-1感染の診断指針 第3版」、HTLV-1水平感染の動向と検査法・検査体制の整備、研究代表者 三浦清徳、研究分担・協力者 渡邉俊樹、内丸薫、山野嘉久、相良康子、鴨居功樹、宇都宮與、久保田龍二、 石塚賢治、根路銘安仁、長谷川寛雄、梅北邦彦、橋倉悠輝、野坂生郷、緒方正男、高起良、大隈和、滝麻衣、長谷川ゆり、倉光球、2023-2025年度 AMED.