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Phosphorylation of RAF
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authored
[InstanceEdit:5658468] Rothfels, Karen, 2014年12月18日
catalystActivity
[CatalystActivity:5672970] protein serine/threonine/tyrosine kinase activity of RAF activating kinases [plasma membrane]
category
transition
compartment
[Compartment:876] plasma membrane
created
[InstanceEdit:5672968] Rothfels, Karen, 2015年02月06日
dbId
5672969
deleted
[Deleted:6798807] Deletion of instance: 109829
[Deleted:9619823] Deletion of instance: 442726
displayName
Phosphorylation of RAF
edited
[InstanceEdit:5674528] Rothfels, Karen, 2015年02月12日
eventOf
[Pathway:R-HSA-5673000] RAF activation - Homo sapiens
followingEvent
[Reaction:R-HSA-5672972] MAP2Ks and MAPKs bind to the activated RAF complex - Homo sapiens
input
4 ×
[SimpleEntity:R-ALL-113592] ATP [cytosol]
[Complex:R-HSA-5672733] p21 RAS:GTP:homo/heterodimerized RAF complex [plasma membrane]
isChimeric
false
isInDisease
false
isInferred
false
literatureReference
[LiteratureReference:5672441] Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding
[LiteratureReference:5672419] Activation of B-Raf kinase requires phosphorylation of the conserved residues Thr598 and Ser601
[LiteratureReference:5672502] Positive regulation of A-RAF by phosphorylation of isoform-specific hinge segment and identification of novel phosphorylation sites
[LiteratureReference:5672246] Raf family kinases: old dogs have learned new tricks
[LiteratureReference:5672474] Mechanistic principles of RAF kinase signaling
[LiteratureReference:5672158] Allosteric activation of functionally asymmetric RAF kinase dimers
[LiteratureReference:5607362] Ras recruits Raf-1 to the plasma membrane for activation by tyrosine phosphorylation
[LiteratureReference:5672435] Differential regulation of Raf-1, A-Raf, and B-Raf by oncogenic ras and tyrosine kinases
[LiteratureReference:5672494] Phosphorylation of Raf-1 serine 338-serine 339 is an essential regulatory event for Ras-dependent activation and biological signaling
[LiteratureReference:5672489] Phosphorylation of 338SSYY341 regulates specific interaction between Raf-1 and MEK1
[LiteratureReference:5607113] Raf-1 serine 338 phosphorylation plays a key role in adhesion-dependent activation of extracellular signal-regulated kinase by epidermal growth factor
[LiteratureReference:5672542] Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation
[LiteratureReference:5672554] The cytokine-activated tyrosine kinase JAK2 activates Raf-1 in a p21ras-dependent manner
[LiteratureReference:5672568] The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338
[LiteratureReference:5672492] Phosphorylation of Raf-1 by p21-activated kinase 1 and Src regulates Raf-1 autoinhibition
[LiteratureReference:5672173] B-Raf and Raf-1 are regulated by distinct autoregulatory mechanisms
[LiteratureReference:5672536] Regulation of the protein kinase Raf-1 by oncogenic Ras through phosphatidylinositol 3-kinase, Cdc42/Rac and Pak
[LiteratureReference:5675130] Regulation of RKIP binding to the N-region of the Raf-1 kinase
[LiteratureReference:5675167] The RKIP (Raf-1 Kinase Inhibitor Protein) conserved pocket binds to the phosphorylated N-region of Raf-1 and inhibits the Raf-1-mediated activated phosphorylation of MEK
[LiteratureReference:5675123] Positive- and negative-feedback regulations coordinate the dynamic behavior of the Ras-Raf-MEK-ERK signal transduction pathway
[LiteratureReference:4420073] Calcium/calmodulin-dependent protein kinase II (CaMKII) phosphorylates Raf-1 at serine 338 and mediates Ras-stimulated Raf-1 activation
maxDepth
1
modified
[InstanceEdit:10778922] Weiser, Joel, 2025年08月27日
name
Phosphorylation of RAF
orthologousEvent
[Reaction:R-MMU-5672969] Phosphorylation of RAF - Mus musculus
[Reaction:R-RNO-5672969] Phosphorylation of RAF - Rattus norvegicus
[Reaction:R-CFA-5672969] Phosphorylation of RAF - Canis familiaris
[Reaction:R-BTA-5672969] Phosphorylation of RAF - Bos taurus
[Reaction:R-SSC-5672969] Phosphorylation of RAF - Sus scrofa
[Reaction:R-GGA-5672969] Phosphorylation of RAF - Gallus gallus
[Reaction:R-DME-5672969] Phosphorylation of RAF - Drosophila melanogaster
output
4 ×
[SimpleEntity:R-ALL-29370] ADP [cytosol]
[Complex:R-HSA-5672718] p21 RAS:GTP:activated RAF homo/heterodimer complexes [plasma membrane]
precedingEvent
[Reaction:R-HSA-5672966] RAS:GTP:'activator' RAF homo/heterodimerizes with other RAF monomers - Homo sapiens
releaseDate
2015年06月17日
reviewStatus
[ReviewStatus:9821382] five stars
reviewed
[InstanceEdit:5690572] Roskoski, Robert Jr, 2015年04月28日
schemaClass
Reaction
species
[Species:48887] Homo sapiens
stId
R-HSA-5672969
summation
[Summation:5672612] Downstream of RAF dimerization and allosteric activation, RA...
updateTrackers
[UpdateTracker:9783842] Update Tracker - [Reaction:5672969] Phosphorylation of RAF - v55:[modifyText]
[UpdateTracker:9778924] Update Tracker - [Reaction:5672969] Phosphorylation of RAF - v67:[addLiteratureReference, modifyText]
Referrals
(precedingEvent)
[Reaction:R-HSA-5672972] MAP2Ks and MAPKs bind to the activated RAF complex
(hasEvent)
[Pathway:R-HSA-5673000] RAF activation
(normalReaction)
[Reaction:R-HSA-6802924] RAF is phosphorylated downstream of oncogenic RAS
[Reaction:R-HSA-6802916] RAF is phosphorylated downstream of moderate kinase activity BRAF mutants
[Reaction:R-HSA-6802941] RAF is paradoxically phosphorylated downstream of kinase-inactive RAF
[Reaction:R-HSA-9656212] Phosphorylation of RAF1 mutants
[Reaction:R-HSA-6802927] BRAF and RAF fusion mutant dimers are phosphorylated
(replacementInstances)
[Deleted:6798807] Deletion of instance: 109829
[Deleted:9619823] Deletion of instance: 442726
(updatedInstance)
[UpdateTracker:9783842] Update Tracker - [Reaction:5672969] Phosphorylation of RAF - v55:[modifyText]
[UpdateTracker:9778924] Update Tracker - [Reaction:5672969] Phosphorylation of RAF - v67:[addLiteratureReference, modifyText]
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