Abstract:PURPOSE: To correlate the morphogenic and molecular traits that affect fungal virulence in human corneas. METHODS: C. albicans wild-type strains SC5314 and VE175 were compared using in vitro growth kinetics, filamentation assays, and microarray analysis. Corneal virulence was assessed ex vivo by inoculating C. albicans onto superficially scarified human corneas that were processed after 1 and 3 days to measure hyphal penetration. For comparison, DSY459, a C. albicans homozygous deletion mutant deficient in secreted aspartyl proteinases (SAP) 4, 5, and 6, was evaluated. RESULTS: C. albicans strain SC5314 was highly filamentous in vitro and more invasive in human corneal explants while VE175 demonstrated limited filamentation and less corneal invasion. Among 6,655 C. albicans genes, 9.0% significantly (p<.05) differed by 2 fold or more between SC5314 and VE175. Genes involved in fungal filamentation that were upregulated in strain SC5314 compared to VE175 included SAP5, SAP6, and other hypha-associated genes. Compared to wild-type strains, DSY459 had intermediate filamentation and stromal penetration. CONCLUSIONS: Fungal genes involved in filamentation likely contribute to virulence differences between wild-type strains of C. albicans. The corneal pathogenicity of C. albicans involves the morphogenic transformation of yeasts into hyphae.

Status: Published Type: Journal Article PubMed ID: 19649176

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#1-10(AAP1-ALS4) #11-20(ALS7-ASR1) #21-30(ATX1-CCP1) #31-40(CDC34-CTN1) #41-50(CWH8-EFG1) #51-60(ENA2-FGR13) #61-70(FGR14-GAD1) #71-80(GCA1-HAP3) #81-90(HBR2-HXT5) #91-100(HYR1-KRE6) #101-110(LAP3-MXR1) #111-120(NCE103-PGA31) #121-130(PGA34-PHO87) #131-140(PHO89-RAS2) #141-150(RBE1-SAM2) #151-160(SAP10-SMC1) #161-170(SMC3-THR4) #171-180(TIF11-YWP1) #181-181(ZRT2-ZRT2)

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#1-10(AAP1-ALS4) #11-20(ALS7-ASR1) #21-30(ATX1-CCP1) #31-40(CDC34-CTN1) #41-50(CWH8-EFG1) #51-60(ENA2-FGR13) #61-70(FGR14-GAD1) #71-80(GCA1-HAP3) #81-90(HBR2-HXT5) #91-100(HYR1-KRE6) #101-110(LAP3-MXR1) #111-120(NCE103-PGA31) #121-130(PGA34-PHO87) #131-140(PHO89-RAS2) #141-150(RBE1-SAM2) #151-160(SAP10-SMC1) #161-170(SMC3-THR4) #171-180(TIF11-YWP1) #181-181(ZRT2-ZRT2)

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#1-10(AAP1-ALS4) #11-20(ALS7-ASR1) #21-30(ATX1-CCP1) #31-40(CDC34-CTN1) #41-50(CWH8-EFG1) #51-60(ENA2-FGR13) #61-70(FGR14-GAD1) #71-80(GCA1-HAP3) #81-90(HBR2-HXT5) #91-100(HYR1-KRE6) #101-110(LAP3-MXR1) #111-120(NCE103-PGA31) #121-130(PGA34-PHO87) #131-140(PHO89-RAS2) #141-150(RBE1-SAM2) #151-160(SAP10-SMC1) #161-170(SMC3-THR4) #171-180(TIF11-YWP1) #181-181(ZRT2-ZRT2)

• To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  displays other papers with information about that topic for that gene.
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  The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.


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Jump to Summary Chart for:

#1-10(AAP1-ALS4) #11-20(ALS7-ASR1) #21-30(ATX1-CCP1) #31-40(CDC34-CTN1) #41-50(CWH8-EFG1) #51-60(ENA2-FGR13) #61-70(FGR14-GAD1) #71-80(GCA1-HAP3) #81-90(HBR2-HXT5) #91-100(HYR1-KRE6) #101-110(LAP3-MXR1) #111-120(NCE103-PGA31) #121-130(PGA34-PHO87) #131-140(PHO89-RAS2) #141-150(RBE1-SAM2) #151-160(SAP10-SMC1) #161-170(SMC3-THR4) #171-180(TIF11-YWP1) #181-181(ZRT2-ZRT2)

• To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  displays other papers with information about that topic for that gene.
  displays other papers in CGD that are associated with that topic.
  The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.


• To go to the Locus page for a gene, click on the gene name.