Strain Data

Synonym
Strain number		NIES-2587
Phylum		Heterokontophyta
Class		Eustigmatophyceae
Scientific name		Microchloropsis gaditana (L.M.Lubián) M,W.Fawley, I.Jameson & K.P.Fawley
Former name		Nannochloropsis gaditana Lubián
Common name
Locality (Date of collection)		Mediterranean Sea (Spanish Coast), Spain (2001年03月20日)
Latitude / Longitude		41.66666666 / 2.8
Habitat (Isolation source)		Marine (Seawater)
History		< RCC (2009);
Isolator (Date of isolation)		Guillou, L. (2001年03月20日)
Identified by
State of strain		Cryopreservation; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: f/2 Temperature: 20 C Light intensity: 19-24 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		16S rRNA ( LN735429 ) , 18S rRNA ( KT861104 )
Cell size (min - max)		- 3 μm
Organization		Unicellular
Characteristics		Planktonic
Other strain no.		Other collection strain no. : RCC504 Other strain no. : BL_120-7
Remarks		Cryopreserved
Movie

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2700	Gephyrocapsa oceanica	Coccolithophore	Marine (Seawater)	Planktonic
NIES-3369	Triparma sp.	Marine (Seawater)	Non-siliceous flagellated stage[2018 May]
NIES-323	Skeletonema marinoi-dohrnii complex	Centric diatom	Marine (Seawater)	Red tide ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade
NIES-2691	Pelagomonas calceolata	Marine (Seawater)	Planktonic
NIES-2697	Emiliania huxleyi	Coccolithophore	Marine (Seawater)	Planktonic

Reference
del Campo, J., Sieracki, M.E., Molestina, R., Keeling, P., Massana, R., Ruiz-Trillo, I. 2014 The others: our biased perspective of eukaryotic genomes. Trends Ecol. Evol., 29, 252-259. Strain(s): 1000, 2582, 2584, 2587, 2590, 2670, 2673, 2691, 2693, 2694, 2695, 2696, 2697, 2699, 2700, 2707, 2751, 2753, 2755, 2756, 2758, 2761, 3369, 3370, 3371 PubMed: 24726347 DOI: 10.1016/j.tree.201403006 Yamagishi, T., Ota, S., Yamaguchi, H., Koshikawa, H., Tatarazako, N., Yamamoto, H., Kawachi, M. 2019 Ecotoxicological bioassay using marine algae for deep-sea mining. In Environmental Issues of Deep-Sea Mining: Impacts, Consequences and Policy Perspectives, Ed. by Sharma, R., Springer, Cham, pp. 255-271. Strain(s): 323, 613, 969, 971, 981, 1000, 1001, 1310, 1411, 2304, 2587, 2590, 2670, 2672 DOI: 10.1007/978-3-030-12696-4_10 Ota, S., Yamaguchi, H., Vanel, F., Fuchida, S., Koshikawa, H., Yamagishi, T., Yamamoto, H., Kawachi, M. 2020 Differential heavy metal sensitivity in seven algal species from the NIES culture collection based on delayed fluorescence assays. Phycol. Res., 68, 41-49. Keywords: bioassay; delayed fluorescence; metal toxicity; pelagic environment, phytoplankton; seafloor massive sulfide Strain(s): 969, 971, 981, 1001, 1310, 2587, 2670 DOI: 10.1111/pre.12403 Kareya, M. S., Mariam, I., Shaikh, K. M., Nesamma, A. A., Jutur, P. P. 2020 Photosynthetic carbon partitioning and metabolic regulation in response to very-low and high CO2 in Microchloropsis gaditana NIES 2587. Front. Plant Sci., 11, 981 (article ID). Keywords: Microchloropsis; biomass; carbon dioxide; oleaginous microalga; photosynthetic cell factories Strain(s): 2587 PubMed: 32719702 DOI: 10.3389/fpls.2020.00981 Mojzeš, P., Gao, L., Ismagulova, T., Pilátová, J., Moudříková, Š., Gorelova, O., Solovchenko, A., Nedbal, L., Salih, A. 2020 Guanine, a high-capacity and rapid-turnover nitrogen reserve in microalgal cells. Proc. Natl. Acad. Sci. U. S. A., 117, 32722-32730. Strain(s): 2285, 2587, 2860 PubMed: 33293415 DOI: 10.1073/pnas.2005460117 Fitriyah, F., Faramitha, Y., Sari, D. A., Kresnawaty, I., Panji, T., Santoso, D. 2020 Molecular identification of species in genus Nannochloropsis using Ribulose-1,5-Biphosphate carboxylase/oxygenase large subunit (rbcL) gene barcode. Eurasia. J. Biosci., 14, 7855-7860. Keywords: DNA barcode; Microalgae; rbcL Strain(s): 2145, 2587 Fitriyah, F., Faramitha, Y., Sari, D. A., Kresnawaty, I., Panji, T., Santoso, D. 2021 Improved direct lysis PCR amplification method of microalgal culture for sequencing and species identification. IOP Conf. Ser.: Earth Environ. Sci., 948, 012013 (article ID). Strain(s): 2587 DOI: 10.1088/1755-1315/948/1/012013

Reference

del Campo, J., Sieracki, M.E., Molestina, R., Keeling, P., Massana, R., Ruiz-Trillo, I. 2014 The others: our biased perspective of eukaryotic genomes. Trends Ecol. Evol., 29, 252-259.
Strain(s): 1000, 2582, 2584, 2587, 2590, 2670, 2673, 2691, 2693, 2694, 2695, 2696, 2697, 2699, 2700, 2707, 2751, 2753, 2755, 2756, 2758, 2761, 3369, 3370, 3371
PubMed: 24726347
DOI: 10.1016/j.tree.201403006

Yamagishi, T., Ota, S., Yamaguchi, H., Koshikawa, H., Tatarazako, N., Yamamoto, H., Kawachi, M. 2019 Ecotoxicological bioassay using marine algae for deep-sea mining. In Environmental Issues of Deep-Sea Mining: Impacts, Consequences and Policy Perspectives, Ed. by Sharma, R., Springer, Cham, pp. 255-271.
Strain(s): 323, 613, 969, 971, 981, 1000, 1001, 1310, 1411, 2304, 2587, 2590, 2670, 2672
DOI: 10.1007/978-3-030-12696-4_10

Ota, S., Yamaguchi, H., Vanel, F., Fuchida, S., Koshikawa, H., Yamagishi, T., Yamamoto, H., Kawachi, M. 2020 Differential heavy metal sensitivity in seven algal species from the NIES culture collection based on delayed fluorescence assays. Phycol. Res., 68, 41-49.
Keywords: bioassay; delayed fluorescence; metal toxicity; pelagic environment, phytoplankton; seafloor massive sulfide
Strain(s): 969, 971, 981, 1001, 1310, 2587, 2670
DOI: 10.1111/pre.12403

Kareya, M. S., Mariam, I., Shaikh, K. M., Nesamma, A. A., Jutur, P. P. 2020 Photosynthetic carbon partitioning and metabolic regulation in response to very-low and high CO2 in Microchloropsis gaditana NIES 2587. Front. Plant Sci., 11, 981 (article ID).
Keywords: Microchloropsis; biomass; carbon dioxide; oleaginous microalga; photosynthetic cell factories
Strain(s): 2587
PubMed: 32719702
DOI: 10.3389/fpls.2020.00981

Mojzeš, P., Gao, L., Ismagulova, T., Pilátová, J., Moudříková, Š., Gorelova, O., Solovchenko, A., Nedbal, L., Salih, A. 2020 Guanine, a high-capacity and rapid-turnover nitrogen reserve in microalgal cells. Proc. Natl. Acad. Sci. U. S. A., 117, 32722-32730.
Strain(s): 2285, 2587, 2860
PubMed: 33293415
DOI: 10.1073/pnas.2005460117

Fitriyah, F., Faramitha, Y., Sari, D. A., Kresnawaty, I., Panji, T., Santoso, D. 2020 Molecular identification of species in genus Nannochloropsis using Ribulose-1,5-Biphosphate carboxylase/oxygenase large subunit (rbcL) gene barcode. Eurasia. J. Biosci., 14, 7855-7860.
Keywords: DNA barcode; Microalgae; rbcL
Strain(s): 2145, 2587

Fitriyah, F., Faramitha, Y., Sari, D. A., Kresnawaty, I., Panji, T., Santoso, D. 2021 Improved direct lysis PCR amplification method of microalgal culture for sequencing and species identification. IOP Conf. Ser.: Earth Environ. Sci., 948, 012013 (article ID).
Strain(s): 2587
DOI: 10.1088/1755-1315/948/1/012013

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