Strain number NIES-2202
Phylum Chlorophyta
Class Chlorophyceae
Scientific name Chlamydomonas applanata Pringsheim
Synonym
Former name Chlamydomonas aggregata Deason et Bold
Common name Green alga
Locality (Date of collection) Williamson County, Texas, USA
Latitude / Longitude 30.689888 /
Habitat (Isolation source) Terrestrial
History < IAM (2007) < BIU (UTEX; 1967);
Isolator (Date of isolation) Deason, T. R. (1957-**-**)
Identified by
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Jan]
Culture condition
(Preculture condition)
Medium: C (agar)
Temperature: 20 C
Light intensity: 3-5 µmol photons/m2/sec, L/D cycle: 10L:14D
Duration: 6 M
Gene information Whole-genome ( BDCZ00000000 ) , 18S rRNA ( AB701514 )
Cell size (min - max) 2 - 10 μm
Organization Unicellular; Flagellate
Characteristics Phototaxis ; Genome decoded strain (Hirashima et al. 2016)
Other strain no. Other collection strain no. : IAM C-214; SAG 2.72; UTEX 969
Other strain no. : Deason T-1-12
Remarks Cryopreserved; Axenic
Movie
Related strain information
(Large number of orders and same genus or species)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2235
Chlamydomonas reinhardtii Green alga Phototaxis ; Starch production (Kuchitsu et al. 1988) ; Genome decoded strain ; Mating type (−) ; Crosses with NIES-2239 (IAM C-562) ; Live food (Liu et al. 2016)
NIES-2236
Chlamydomonas reinhardtii Green alga Terrestrial (Soil) Phototaxis ; Mating type (+)
NIES-2237
Chlamydomonas reinhardtii Green alga Phototaxis ; D-Threonine aldolase (Hirato et al. 2007) ; D-amino acids ; Cell wall less mutant of NIES-2238 ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005)
NIES-2238
Chlamydomonas reinhardtii Green alga Terrestrial (Soil) Phototaxis ; Mating type (+) ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005)
NIES-2463
Chlamydomonas reinhardtii Green alga Freshwater (Soil) Phototaxis ; Heterothallic ; Dioecious ; Isogamous ; Mating type (+)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2499
Chlamydomonas eustigma Green alga Freshwater (Microfilm) Acidophilic ; Phototaxis ; Arsenic tolerance (Hirooka et al. 2017) ; Genome decoded strain (Hirooka et al. 2017)
NIES-2212
Chlamydomonas debaryana Green alga Phototaxis ; Starch production (Toyoshima et al. 2015) ; Genome decoded strain (Hirashima et al. 2016)
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2215
Chloromonas difformis Green alga Freshwater (Pond water) Phototaxis
NIES-144
Haematococcus lacustris Green alga Freshwater (Lake water) Astaxanthin production ; Genome decoded strain (Morimoto et al. 2017) ; Homothallic ; Isogamous
NIES-2222
Chlamydomonas moewusii Green alga Phototaxis
NIES-968
Chlamydomonas kuwadae Green alga Freshwater (Lake water) Phototaxis
NIES-362
Pandorina morum Green alga Freshwater (Lake water) Phototaxis ; Heterothallic ; Mating type (−) ; Crosses with NIES-242
Reference
Brand, J. J. & Wright, J. N. 1989 Hydrogen production by eukaryotic algae. Biotech. Bioeng., 33, 1482-1488.
Strain(s): 2151, 2169, 2190, 2194, 2201, 2202, 2203, 2204, 2211, 2213, 2219, 2279, 2291

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158, 437, 438, 440, 884, 968, 1021, 1022, 1048, 1733, 1848, 1849, 2201, 2202, 2203, 2204, 2206, 2207, 2208, 2209, 2210, 2211, 2212, 2213, 2214, 2215, 2216, 2218, 2219, 2220, 2222, 2223, 2224, 2225, 2228, 2229, 2230, 2231, 2233, 2234, 2235, 2236, 2237, 2238, 2239, 2240, 2241, 2242, 2243, 2246, 2247, 2248, 2314, 2315, 2316, 2317, 2318, 2319, 2320, 2321, 2322, 2323, 2324, 2462, 2463, 2499, 2575, 2576, 2577, 2578, 2579, 3284

Sakurai, K., Mori, N., Sato, N. 2014 Detection and characterization of phosphatidylcholine in various strains of the genus Chlamydomonas (Volvocales, Chlorophyceae). J. Plant Res., 127, 641-650.
Keywords: Chlamydomonas; Delta 5 desaturase; Diacylglyceryl-N,N,N-trimethylhomoserine; Fatty acid; Phosphatidylcholine
Strain(s): 2202, 2207, 2212, 2215, 2219, 2225, 2230, 2242, 2316, 2462, 2499
PubMed: 24947506
DOI: 10.1007/s10265-014-0644-0

Hirashima, T., Tajima, N., Sato, N. 2016 Draft genome sequences of four species of Chlamydomonas containing phosphatidylcholine. GenomeA, 4, e01070-16 (article ID).
Strain(s): 2202, 2207, 2212, 2242
PubMed: 27688324
DOI: 10.1128/genomeA.01070-16

Hirashima, T., Toyoshima, M., Moriyama, T., Nakamura, Y., Sato, N. 2017 Characterization of phosphoethanolamine-N-methyltransferases in green algae. Biochem. Biophys. Res. Commun., 488, 141-146.
Keywords: Green algal evolution; Chlamydomonas; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase
Strain(s): 2202, 2207, 2212, 2235, 2242
PubMed: 2848352
DOI: 10.1016/j.bbrc.2017年05月02日6

Hirashima, T., Toyoshima, M., Moriyama, T., Sato, N. 2018 Evolution of the phosphatidylcholine biosynthesis pathways in green algae: Combinatorial diversity of methyltransferases. J. Mol. Evol., 86, 68-76.
Keywords: Chlamydomonas; Metabolic diversity; Phosphatidylcholine biosynthesis; Phosphoethanolamine-N-methyltransferase; Phospholipid-N-methyltransferase
Strain(s): 2202, 2207, 2212, 2242
PubMed: 29330556
DOI: 10.1007/s00239-017-9826-4

Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2019 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol., 55, 118-133.
Keywords: abnormal swimming behavior; cell mortality; cell wall damage; Chlamydomonas rein-hardtii; poly(isobutyl-cyanoacrylate) nanoparticles; ROS
Strain(s): 35, 144, 362, 418, 421, 432, 438, 456, 545, 571, 732, 737, 858, 1022, 1048, 1848, 2150, 2169, 2201, 2202, 2208, 2209, 2212, 2280, 2352, 2462, 2578
PubMed: 30304548
DOI: 10.1111/jpy.12798

Flores, D. C., Fricke, M., Wesp, V., Desirò, D., Kniewasser, A., Hölzer, M., Marz, M., Mittag, M. 2021 A marine Chlamydomonas sp. emerging as an algal model. J. Phycol., 57, 54-69.
Strain(s): 882, 2202, 2204, 2207, 2211, 2218, 2220, 2223, 2229, 2236, 2241, 2242, 2248
PubMed: 33043442
DOI: 10.1111/jpy.13083

Sato, N., Sato, M., Wakazaki, M., Moriyama, T., Hirashima,T., Toyooka, K. 2024 Chloroplasts with clefts and holes: a reassessment of the chloroplast shape using 3D FE-SEM cellular reconstruction of two species of Chlamydomonas. Protoplasma, 262, 207–218.
Keywords: Array tomography; Chlamydomonas; Chloroplast shape; Three-dimensional reconstruction; Lipid droplet topology
Strain(s): 2202
PubMed: 39320475
DOI: 10.1007/s00709-024-01990-7

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