Terminal-based plasmid map viewer, sequence editor, and cloning/mutagenesis workbench in pure Python
Batch primer design for genomic regions
Safe, idiomatic Rust bindings to the primer3 C library for PCR primer design and oligonucleotide thermodynamic calculations
A high-performance Rust implementation of primer3 design, leveraging PyO3 to interface with Python's primer3 package. This tool provides fast and reliable primer design for qPCR applications with thermodynamic filtering.
Species-specific primer design tool: NCBI retrieval → BLAST mismatch analysis → Primer3 design → Primer-BLAST validation. Desktop GUI (PySide6) + PDF/DOCX reports.
WebAssembly build of primer3 for client-side PCR primer and probe design
Focused WSL-backed Primer3 and primer specificity GUI for Windows
Focused WSL-backed exon primer and CAPS marker GUI for Windows
Local, open-source Primer-BLAST for plant breeding: Primer3 + BLAST+, multi-reference specificity, in-silico PCR, CAPS/dCAPS, offline
DesignLab — conservation-aware PCR/qPCR primer design: NCBI search, MAFFT alignment, conserved-block Primer3 design and in-silico validation, with every binding site visualised
Beginner-friendly Streamlit tool for NCBI sequence retrieval, Primer3-based PCR primer design, thermodynamic analysis, and CSV export.
Evidence-traceable Windows desktop app for transposable-element (TE) annotation and TE-aware, genome-validated PCR primer design. Native PySide6/Qt, offline scientific core, optional one-click Dfam/RepeatMasker + minimap2 backend.
Python 3 toolkit for designing specific, unique primer pairs across target and background genomes
A collection of simple python tools for molecular biology and bioinformatics
A web app interface for Primer3 using Streamlit and primer3-py, modeled after Primer3Plus
CE-specific qPCR primer design pipeline: Primer3 + pair-wise BLAST against genome & curated RefSeq mRNA to select junction-spanning assays.
Simple shiny app to analyze primer quality.
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