Nucleic acid QC with the Agilent Fragment Analyzer system
The Fragment Analyzer system accurately assesses quality and quantity of isolated nucleic acids and prepared libraries to optimize cluster generation during sequencing.
Minimize errors, reduce hands-on-time, and enable higher throughput with automation options across Illumina library prep products
Automated liquid-handling systems help labs achieve and maintain consistent results while driving efficiency. NGS library prep automation also allows for the rapid scaling of throughput while reducing hands-on time.
Illumina partners with leading vendors to provide automated protocols for Illumina library prep kits, combining our partners’ automation knowledge with our library prep and sequencing expertise to build an NGS solution that works for you.
This guide walks you through the range of automation tools available for NGS library prep and helps you find a solution tailored for your application, throughput, and budget needs. Learn about top considerations for building your automated library prep protocol.
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Methods are developed by our automation partners with input from Illumina. Illumina reviews sequencing data from these methods to ensure performance and data quality. Our partners own, supply, install, and support these methods. Our partners service and support their liquid handler systems.
Illumina co-develops and tests the method with our automation partners. Our partners own, supply, and install these methods. Illumina provides front-line field and technical support for these methods.* Our partners provide secondary support for these methods. Our partners service and support their liquid handler systems.
These methods are developed and tested by our automation partners. Illumina does not review the data from these methods. Our partners own, install, and support these methods. Our partners service and support their liquid handler systems.
*Support levels may vary by region. Contact your local support team for more information.
See which library prep kits are automated on which liquid handling platforms, or find Illumina Qualified and/or partner-developed methods for a given platform.
| Beckman Coulter | Eppendorf | Hamilton | Revvity | Tecan | SPT Labtech | |
| AmpliSeq for Illumina** |
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NGS STAR NGS STARlet |
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| AmpliSeq for Illumina Cancer HotSpot Panel v2** |
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Sciclone G3 NGSx | |||
| AmpliSeq for Illumina Comprehensive Panel v3** |
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| AmpliSeq for Illumina Focus Panel** |
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Sciclone G3 NGSx | |||
| COVIDSeq Assay (96 samples) | Biomek i7 | epMotion 5075t | NGS STAR |
Zephyr G3 NGS |
Fluent DreamPrep | |
| COVIDSeq Test (RUO Version) | epMotion 5075t | NGS STAR |
Zephyr G3 NGS |
Fluent DreamPrep | ||
| Illumina Complete Long Read Prep, Human | epMotion 5075t | |||||
| Illumina DNA PCR-Free Prep | Biomek i5
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Fontus NGS
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| Illumina DNA Prep | Biomek i5
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Zephyr G3 NGS |
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| Illumina DNA Prep with Enrichment |
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DreamPrep | |
| Illumina DNA Prep with Exome 2.5 Enrichment |
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NGS STAR
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| Illumina RNA Prep with Enrichment | Biomek i5
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NGS STAR |
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| Illumina Stranded mRNA Prep |
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DreamPrep | ||
| llumina Stranded Total RNA Prep |
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DreamPrep | ||
| Nextera XT DNA |
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Zephyr G3 NGS |
DreamPrep | |
| Pillar Biosciences oncoReveal Solid Tumor v2 Gene Panel Kit |
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| TruSeq DNA Nano |
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Sciclone G3 NGSx |
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| TruSeq DNA PCR-Free |
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NGS STAR |
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| TruSeq Stranded mRNA |
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Zephyr G3 NGS |
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| TruSeq Stranded Total RNA |
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Zephyr G3 NGS |
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| TruSight Oncology 500 | Biomek i5
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epMotion 5075t |
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DreamPrep | |
| TruSight Oncology 500 ctDNA v2 |
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| TruSight Oncology 500 (DNA Only) | Biomek i5 Biomek i7
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| TruSight Oncology 500 High-Throughput |
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DreamPrep | |||
| TruSight RNA Pan-Cancer Panel |
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epMotion 5075t | ||||
| TruSight Tumor 15 |
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| TruSight Tumor 170 |
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Starting in January 2022, Hamilton will refer to the NGS STAR configuration for TruSight Oncology library preparations as the SBS STAR (LSA). All existing Illumina Qualified methods are backwards compatible and will continue to run on this system as this change is in name only.
** In order to accommodate for higher dead volume requirements associated with automated platforms and variation in overfill volumes by the original reagent manufacturer, more than one AmpliSeq for Illumina kit may be required when preparing the maximum number of libraries per kit.
Track samples, manage your runs, and launch into production with preset laboratory information management system (LIMS) protocols. Leverage a compatible robotic liquid handler to prepare libraries for rapid high-throughput sequencing and data analysis.
Laboratory information management system designed to track samples and manage workflows for an optimized and efficient lab.
Sequencing systems delivering up to 16 Tb of output, allowing sequencing of 128 human genomes at ×ばつ coverage in a single run.
Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.
Illumina DRAGEN secondary analysis
Accurate, comprehensive, and efficient secondary analysis of next-generation sequencing data.
A comprehensive research software platform to support tertiary analysis clinical research workflows.
The Fragment Analyzer system accurately assesses quality and quantity of isolated nucleic acids and prepared libraries to optimize cluster generation during sequencing.
This streamlined automated workflow generates highly uniform libraries and provides excellent data for species identification, metagenomic profiling, and de novo genome assembly.
COVIDSeq Assay and COVIDSeq Test (RUO) workflows are compatible with a range of automation solutions and configurable for batch sizes from 8–96 samples.
Automation workflow for Illumina DNA Prep with Exome 2.5 Enrichment
This workflow automates library preparation and enrichment on the Hamilton SBS STARlet Automated Liquid Handling Platform with consistent, high-quality data with less hands-on time, compared to the manual workflow.
Contact us to learn more about automation partners and Illumina Qualified methods.
Your email address is never shared with third parties.