Designing Your Experimental Protocol
These recommendations and best practices are designed to help you ensure the success of your next-generation sequencing (NGS) experiment. Review key considerations, such as calculating read length, estimating coverage, and measuring sequencing accuracy. Use our interactive tools to help you create an NGS protocol or select the right products and methods for your project.
Jump-Start Your NGS Experiment
Want to get started faster? Consult with experimental design experts through our Workflow Design and Evaluation Service.* We’ll help you design a workflow that’s right for you, process your samples, and generate your first NGS data set.
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Contact Us Learn MoreOther NGS Experimental Considerations
Library Quantification
Sequencing Quality
Planning an Experimental Budget
As next-generation sequencing costs continue to decline, Illumina is leading the way in making NGS more affordable and accessible. With these resources, we’ll guide you through key factors to consider when planning your NGS budget.
Learn MoreCreate Your NGS Protocol
A fast, flexible, and mobile-friendly tool, our Custom Protocol Selector helps you generate next-generation sequencing protocols tailored to your experiment.
Learn MoreAdditional Resources
Experiment Planning Tools
Find interactive tools designed to help you choose the right methods and products for your needs.
How to Plan Your First NGS Experiment
Experimental considerations and workflow resources for your sequencing project.
Technical Bulletins
NGS experimental design tips and resources from our Technical Support team.
Online Training
View all our training courses or sign up for upcoming webinars.