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1 Fixation

2 Washing in tap water

3 Dissecting Brains

4 Numbering

5 Dehydration

6 Clearing

7 Paraffin impregnation

8 Paraffin embedding

9 Mounting on a wooden block

10 Sectioning

11 Deparaffinization & hydration

12 Staining

13 Dehydration, clearing and coverslipping

3 Dissecting Brains

Dissect the brain tissue to the size of a paraffin block.

Method

  • Perform the dissecting procedure on a workbench equipped with a down-flow push-pull ventilation system to vent formalin fumes.
  • Equipment for dissecting
  • Photography stand for imaging the dissecting tissue.
  • Set up photocatalyst environmental purification equipment to deal with the formalin.

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