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Table of Contents

July 2025

Cover

Florida Triatoma sanguisuga adult female (left) with 1st instar (top right) and 5th instar nymphal stages collected at a Florida home. Beatty & Bhosale et al, 2025

Image Credit: Nathan Burkett-Cadena

Viewpoints

Large language models and their performance for the diagnosis of histoplasmosis

Mathieu Nacher, Ugo Françoise, Antoine Adenis

PLOS Neglected Tropical Diseases: published July 9, 2025 | https://doi.org/10.1371/journal.pntd.0013151

Engaging communities in the control of arboviral diseases: insights from the African region

Anankpo Gildas Yahouédo, Corinne S. Merle, Emmanuel Chanda, Ingrid B. Rabe, Florence Fouque, Diana P. Rojas, Abraham Aseffa, Christine M. Halleux, Raman Velayudhan

PLOS Neglected Tropical Diseases: published July 15, 2025 | https://doi.org/10.1371/journal.pntd.0013300

Country-level indices in predictive models of helminth infections: Perspectives from Southeast Asia

Nathkapach Kaewpitoon Rattanapitoon, Chutharat Thanchonnang, Schawanya Kaewpitoon Rattanapitoon

PLOS Neglected Tropical Diseases: published July 18, 2025 | https://doi.org/10.1371/journal.pntd.0013330

Cholera crisis persists: A call for integrated health strategies

Musawer Khan, Kamil Ahmad Kamil

PLOS Neglected Tropical Diseases: published July 23, 2025 | https://doi.org/10.1371/journal.pntd.0013272

Reviews

Trend analysis and spatiotemporal distribution of leishmaniasis disease incidence in Sri Lanka: A detailed review from 2009 to 2023

Nayana Gunathilaka, Deshaka Jayakody, Rajitha Wickremasinghe

PLOS Neglected Tropical Diseases: published July 2, 2025 | https://doi.org/10.1371/journal.pntd.0013158

Comprehensive analysis of Toxoplasma gondii migration routes and tissue dissemination in the host

Carlos J. Ramírez-Flores, Ricardo Mondragón-Flores

PLOS Neglected Tropical Diseases: published July 31, 2025 | https://doi.org/10.1371/journal.pntd.0013369

Research Articles

Safety and tolerability of metformin in overweight and obese patients with dengue: An open-label clinical trial (MeDO)

Nguyet Minh Nguyen, Dong Thi Hoai Tam, Ho Quang Chanh, Huynh Trung Trieu, Nguyen Thi Xuan Chau, Nguyen Thanh Van, Nguyen Thanh Phong, Cao Thi Tam, Nguyen Thi Hong Lan, Luong Thi Hue Tai, Nguyen Thi Cam Huong, Le Thi Ngoc Thuan, Nguyen Van Hao, Phan Tu Qui, Tran Thi Dong Vien, Pham Thi Hai Men, Huynh Thi Thuy Hoa, Phan Vinh Tho, Huynh Thi Le Duyen, Vi Tran Thuy, Thuan Dang Trong, Nguyen Tan Thanh Kieu, Angela McBride, Evelyne Kestelyn, Ronald B. Geskus, Nguyen Lam Vuong, Sophie Yacoub

PLOS Neglected Tropical Diseases: published July 1, 2025 | https://doi.org/10.1371/journal.pntd.0013281

Assessment of the effectiveness of public art in improving knowledge, attitude, practices and mitigation of stigmatization regarding neglected tropical diseases in South Eastern, Nigeria

Uchechukwu M. Chukwuocha, Ayoola O. Bosede, Chidubem D. Osuji, Adanna N. Chukwuocha, Harriet Abugewa, Amarachi Amawuru, Adesua Okoroh, Akuchi Echefula, Precious Nwabueze, Nathan Ukwenga, Alfred Barile

PLOS Neglected Tropical Diseases: published July 1, 2025 | https://doi.org/10.1371/journal.pntd.0013266

Tracking progress along the WHO Neglected Tropical Diseases Road Map to 2030: A guide to the Gap Assessment Tool (GAT) and results from the 2023–2024 assessment

Meritxell Donadeu, Theresa W. Gyorkos, Olaf Horstick, Patrick Lammie, Upendo J. Mwingira, Wyckliff P. Omondi, Joanna M. Pritchard, Jordan Tappero, YuYen L. Chan, Sophie Welsche, Junerlyn Farah V. Agua, Serge Madjou, Pamela S. Mbabazi, Supriya Warusavithana, Carlos A. Torres-Vitolas

PLOS Neglected Tropical Diseases: published July 1, 2025 | https://doi.org/10.1371/journal.pntd.0013194

Genetic structuring and estimation of reproductive adults in Onchocerca volvulus: A genome-wide analysis across hosts and regions

Pawan Kumar, Young-Jun Choi, Kerstin Fischer, Shannon M. Hedtke, Anusha Kode, Nicholas Opoku, Lincoln Gankpala, Tony O. Ukety, Jöel Lonema Mande, Timothy J.C. Anderson, Warwick N. Grant, Peter U. Fischer, Makedonka Mitreva

PLOS Neglected Tropical Diseases: published July 1, 2025 | https://doi.org/10.1371/journal.pntd.0013221

Effects of an integrated intervention on schistosomiasis prevalence in a rural area of Tanzania

Yoonho Cho, Jungim Lee, Humphrey Deogratias Mazigo, Leah Elisha Salamba, Seungman Cha

PLOS Neglected Tropical Diseases: published July 2, 2025 | https://doi.org/10.1371/journal.pntd.0013215

Treatment outcomes of multi-drug-resistant and rifampicin-resistant tuberculosis with and without isolation of nontuberculous mycobacteria between 2018–2021: A retrospective cohort study in Ghana

Elizabeth Tabitha Abbew, Roger Laryea, Ama Owusuaa Kwakye, Yaw Adusi Poku, Dorcas Obiri-Yeboah, Lutgarde Lynen, Tom Decroo, Leen Rigouts, Natalie Lorent

PLOS Neglected Tropical Diseases: published July 2, 2025 | https://doi.org/10.1371/journal.pntd.0013204

Aedes aegypti ecology and dengue infection in three agricultural areas of Côte d’Ivoire

Edwige M. A. Y. Kadjo, Sylla Yahaya, Négnorogo Guindo-Coulibaly, Valery E. Adjogoua, Danielle D. Zoh, François D. Traoré, Fabrice K. Assouho, Mardoché R. K. Azongnibo, Stéphane D. M. Kpan, Ahoua Yapi, Fabrice Chandre, Maurice A. Adja

PLOS Neglected Tropical Diseases: published July 3, 2025 | https://doi.org/10.1371/journal.pntd.0013255

Genetic diversity of Murray Valley encephalitis virus 1951–2020 identified via phylogenetic and evolutionary analyses

Timo Ernst, Alice Michie, Chisha Sikazwe, Jay Nicholson, Avram Levy, I.-Ly Joanna Chua, John S. Mackenzie, David W. Smith, Allison Imrie

PLOS Neglected Tropical Diseases: published July 3, 2025 | https://doi.org/10.1371/journal.pntd.0013181

Detecting soil-transmitted helminth and Schistosoma mansoni eggs in Kato-Katz stool smear microscopy images: A comprehensive in- and out-of-distribution evaluation of YOLOv7 variants

Mohammed Aliy Mohammed, Esla Timothy Anzaku, Peter Kenneth Ward, Bruno Levecke, Janarthanan Krishnamoorthy, Wesley De Neve, Sofie Van Hoecke

PLOS Neglected Tropical Diseases: published July 3, 2025 | https://doi.org/10.1371/journal.pntd.0013234

Optimizing dog population control strategies in Thailand using mathematical and economic modeling

Weerakorn Thichumpa, Anuwat Wiratsudakul, Saranath Lawpoolsri, Yanin Limpanont, Weerapong Thanapongtharm, Lauren M. Smith, Sarawut Maneewong, Wirichada Pan-ngum

PLOS Neglected Tropical Diseases: published July 3, 2025 | https://doi.org/10.1371/journal.pntd.0013202

Assessing the burden of Scorpionism: Epidemiological trends and health outcomes in Northwest of Iran

Madineh Abbasi, Mehran Shahi, Hossein Barahoei, Ahmad Ali Hanafi-Bojd, Ali Banagozar Mohammadi, Mahasti Alizadeh, Mostafa Farmani, Simin Khayatzadeh, Karim Gerami, Abdollah Badzohreh, Aida Amirijavid, Saideh Yousefi

PLOS Neglected Tropical Diseases: published July 3, 2025 | https://doi.org/10.1371/journal.pntd.0013201

Brucellosis relapse: Rate, patient characteristics, and clinical outcomes in an endemic region

Ibrahim Bahabri, Anadel Hakeem, Mohammed S. Alturki, Mutaz Althobaiti, Sameera Aljohani, Ahmad Alharbi, Mohammad Bosaeed

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0013270

Integrated vector management with the sterile insect technique component for the suppression of Aedes aegypti in an urban setting in Indonesia

Hadian Iman Sasmita, Kok-Boon Neoh, Beni Ernawan, Murni Indarwatmi, Indah Arastuti Nasution, Nur Fitrianto, Tri Ramadhani, Tri Isnani, Yorianta Hidayat Sasaerila, Rafa Listyani Rahman, Sri Yusmalinar, Ramadhani Eka Putra, Intan Ahmad, Wu-Chun Tu

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0013290

Cardiac alterations induced by Trypanosoma cruzi extracellular vesicles and immune complexes

Alberto Cornet-Gomez, Francisco O’Valle, José M. Garrido, Fernando Rodríguez Serrano, Ana I. Nieto, Antonio Osuna

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0013273

The frequency, clinical characteristics and outcomes of Naja species related injuries in Malaysia consulted to Remote Envenomation Consultancy Services from 2020–2023

Ahmad Khaldun Ismail, Zhi Xuan Ng, Syahirah Rezwan Eskandar, Hamelda Tanisha Ganaprakasam, Zainalabidin Mohamed Ismail

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0013271

Associations between podoconiosis and pedogenic factors globally – A systematic review

Leo Stallard, James Watson, Matthew Brolly, Tegist Chernet, Wendemagegn Enbiale, Ferenc Molnár, Gezahegn Yirgu, Gail Davey

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0013294

Field evidence of Trypanosoma cruzi infection, diverse host use and invasion of human dwellings by the Chagas disease vector in Florida, USA

Norman L. Beatty, Chanakya R. Bhosale, Zoe S. White, Carson W. Torhorst, Kristen N. Wilson, Rayann Dorleans, Tanise M.S. Stenn, Keswick C. Killets, Rachel Curtis-Robles, Nathan Burkett-Cadena, Eva Nováková, Gabriel L. Hamer, Sarah A. Hamer, Samantha M. Wisely

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0012920

Schistosoma japonicum cathepsin L1: A potential target for anti-schistosomiasis strategies

Xianyu Piao, Yuanlong Wang, Ning Jiang, Pengfei Cai, Jiamei Duan, Shuai Liu, Qijun Chen, Nan Hou

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0013241

A mixed-method study to improve outcome of mass drug administration in two TAS failed districts of Uttar Pradesh, India

Achintya Srivatsa, Padmalochan Biswal, Satyabrata Routray, Shweta Prasad, Kevin Bardosh, Paresh Kumar, Amresh Kumar, Aurpit Patnaik, Shoaib Anwar, Vindu Prakash Singh

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0013112

Antitrypanosomal therapy for Chagas disease: A single center experience with adverse drug reactions and strategies for enhancing treatment completion

Katherine Reifler, Alyse Wheelock, Samantha M. Hall, Madolyn Dauphinais, Samuel Roytburd, Michael Maiullari, Alejandra Salazar, Ashley Maldonado, Helen Mahoney West, Julia R. Köhler, Elizabeth D. Barnett, Deepa M. Gopal, Davidson H. Hamer, Daniel L. Bourque

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0013218

Target product profiles for new diagnostics to inform strongyloidiasis control programs

Adama Kazienga, Luc E. Coffeng, Sara Roose, Sake J. de Vlas, Dora Buonfrate, Salvatore Scarso, Francesca Tamarozzi, Bruno Levecke

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0012774

Determining genotype and antimicrobial resistance of Salmonella Typhi in environmental samples by amplicon sequencing

Catherine Troman, Samuel T. Horsfield, Dilip Abraham, Venkata Raghava Mohan, Sidhartha Giri, Satheesh Nair, Alexander G. Shaw, Zoe Dyson, Kathryn E. Holt, Nicholas C. Grassly

PLOS Neglected Tropical Diseases: published July 8, 2025 | https://doi.org/10.1371/journal.pntd.0013211

Prevalence of Leishmania infection in refugee camps: A serological and molecular study in Gambella and Benishangul-Gumuz, Ethiopia

Habtamu Belay, Adugna Abera, Esayas Aklilu, Bortola Abdisa, Mahlet Belachew, Heven Sime, Myrthe Pareyn, Tesfahun Bishaw, Saskia van Henten, Johan van Griensven, Geremew Tasew, Berhanu Erko

PLOS Neglected Tropical Diseases: published July 8, 2025 | https://doi.org/10.1371/journal.pntd.0013280

Functional characterization of a common XIa inhibitory Kunitz-domain Shp4 from nine schistosoma secreted proteins with diverse C-terminal fragments

Xiangdong Ye, Xiaolin Deng, Huanhuan Gao, Shuwen Chen, Li Ding, Fang Sun, Chenhu Qin, Hongyi Luo, Wen Zhu, Xudong Luo, Zongyun Chen

PLOS Neglected Tropical Diseases: published July 8, 2025 | https://doi.org/10.1371/journal.pntd.0013282

Neurocysticercosis in Ecuador: Spatial clustering, social determinants, and epidemiological trends (2017–2023)

Andrés Fernando Vinueza-Veloz, Marlon Fabricio Calispa-Aguilar, Pamela Vinueza-Veloz, Tannia Valeria Carpio-Arias, Jefferson Santiago Piedra-Andrade, María Fernanda Vinueza-Veloz, Belkys Galindo-Santana

PLOS Neglected Tropical Diseases: published July 8, 2025 | https://doi.org/10.1371/journal.pntd.0012205

Forecasting high-risk areas for dengue outbreaks in China: A trend analysis of Aedes albopictus and Aedes aegypti distributions from 2014 to 2030

Yulun Xie, Bingbing Wang, Qi Chen, Hongjie Wei, Yanshu Ke, Fang Xie, Xuhua Guan, Jia Rui, Tianmu Chen

PLOS Neglected Tropical Diseases: published July 9, 2025 | https://doi.org/10.1371/journal.pntd.0013237

Benefits of public awareness in mitigating cystic echinococcosis risk in Western China: A climate and socio-economic perspective

Fang Yin, Wenrui Meng, Peiwei Fan, Yue Shi, Shuai Chen, Yongchun Liang, Jianyi Yao, Yeping Wang, Chuizhao Xue, Shuai Han, Mengmeng Hao, Qian Wang, Ze Meng, Jun Zhuo, Kai Sun, Yongqing Bai, Tingting Kang, Zhenyu Wang, Lei Liu, Dong Jiang, Liqun Fang, Canjun Zheng, Jiping Dong, Fangyu Ding, Tian Ma

PLOS Neglected Tropical Diseases: published July 9, 2025 | https://doi.org/10.1371/journal.pntd.0013182

Mapping the risk of Zika virus infections in pregnant persons and microcephaly in newborns in relation to socioeconomic indicators in Recife, Pernambuco, Brazil: A spatial analysis (2015 to 2021)

Ana Carolyne de Carvalho Lucena Sá, André Luiz Sá de Oliveira, Demócrito de Barros Miranda-Filho, Celina Maria Turchi Martelli, Thalia Velho Barreto de Araújo, Elizabeth B. Brickley, Ricardo Arraes de Alencar Ximenes, Ulisses Ramos Montarroyos

PLOS Neglected Tropical Diseases: published July 9, 2025 | https://doi.org/10.1371/journal.pntd.0013240

Zika virus-specific and orthoflavivirus-cross-reactive IgGs correlate with Zika virus seroneutralization depending on prior dengue virus infection

Solène Marquine, Sébastien Briolant, Damien Claverie, Jessica Denis, Manon Geulen, Laurent Bosio, Bernard Tenebray, Sarah Attoumani, Annabelle Garnier, Laurence Cheutin, Gilda Grard, Bruno Coutard, Guillaume A. Durand, Cyril Badaut

PLOS Neglected Tropical Diseases: published July 9, 2025 | https://doi.org/10.1371/journal.pntd.0013274

Estimating the incidence of dengue in international air travelers from non-endemic countries between 2010–2019

Matt D. T. Hitchings, Yi Xu, Bernardo García-Carreras, Adriana Gallagher, Justin J. O’Hagan, Derek A. T. Cummings

PLOS Neglected Tropical Diseases: published July 9, 2025 | https://doi.org/10.1371/journal.pntd.0013291

Vector control for Aedes aegypti and Aedes albopictus mosquitoes implemented in the field in sub-Saharan Africa: A scoping review

Luciana Lepore, Veerle Vanlerberghe, Kristien Verdonck, Emery Metelo, Mawlouth Diallo, Wim Van Bortel

PLOS Neglected Tropical Diseases: published July 9, 2025 | https://doi.org/10.1371/journal.pntd.0013203

Healthcare workers’ priorities of WHO snakebite strategic objectives for the control and prevention of snakebite envenoming in Ghana: A machine learning statistical design of experiment modeling

Eric Nyarko, Iddrisu Abugbil Atubiga, Emmanuel Tetteh Siame, José María Gutiérrez, Eduardo Alberto Fernandez

PLOS Neglected Tropical Diseases: published July 10, 2025 | https://doi.org/10.1371/journal.pntd.0013295

Traditional medicine use among rabies exposed individuals in Ethiopia: A systematic review and meta-analysis

Gizew Dessie Asres, Yeshiwork Kebede Gessesse, Desalew Salew, Negese Sewagegn Semie, Etsegenet Kindie, Molalign Tarekegn Minwagaw, Wudu Tafere, Zewudu Belay, Habtamu Alebachew, Getasew Mulat Bantie, Taye Zeru

PLOS Neglected Tropical Diseases: published July 11, 2025 | https://doi.org/10.1371/journal.pntd.0013319

Suppression of Aedes aegypti by the sterile insect technique on Captiva Island, Florida, USA from 2020 to 2022

Rachel Morreale, Danilo O. Carvalho, Steven Stenhouse, Johanna Bajonero, Rui Pereira, Daniel A. Hahn, Aaron Lloyd, David F. Hoel

PLOS Neglected Tropical Diseases: published July 11, 2025 | https://doi.org/10.1371/journal.pntd.0013256

Reactive case detection can improve the efficiency of lymphatic filariasis surveillance compared to random sampling, Samoa 2023

Helen J. Mayfield, Benn Sartorius, Angus McLure, Stephanie J. Curtis, Beatris Mario Martin, Sarah Sheridan, Robert Thomsen, Rossana Tofaeono-Pifeleti, Satupaitea Viali, Patricia M. Graves, Colleen L. Lau

PLOS Neglected Tropical Diseases: published July 11, 2025 | https://doi.org/10.1371/journal.pntd.0012622

Complexity of schistosome vector bulinine snails in Kenya: Insights from nuclear genome size variation, complete mitochondrial genome sequence, and morphometric analysis

Si-Ming Zhang, Coen M. Adema, Mohamed R. Habib, Abdelmalek Lekired, Marijan Posavi, Martina R. Laidemitt, Geoffrey M. Maina, Ibrahim N. Mwangi, Joseph M. Kinuthia, Martin W. Mutuku, Eric S. Loker

PLOS Neglected Tropical Diseases: published July 14, 2025 | https://doi.org/10.1371/journal.pntd.0013305

Genetic analysis of IFNG-AS1 implicates opposite effects to Leishmania guyanensis-cutaneous leishmaniasis: rs4913269 confers protection while rs7134599 enhances susceptibility and correlates with high plasma IL-4 and IL-10 levels

Marcus Vinitius de Farias Guerra, Josué Lacerda de Souza, Lener Santos da Silva, José do Espírito Santo Júnior, Tirza Gabrielle Ramos de Mesquita, Krys Layane Guimarães Duarte Queiroz, George Allan Villarouco da Silva, Mauricio Morishi Ogusku, Mara Lúcia Gomes de Souza, José Pereira de Moura Neto, Aya Sadahiro, Rajendranath Ramasawmy

PLOS Neglected Tropical Diseases: published July 14, 2025 | https://doi.org/10.1371/journal.pntd.0013318

Community perceptions of yellow fever and its treatment practices in regions with reported outbreaks in Uganda: A qualitative study

Lena Huebl, Aloysious Nnyombi, Patricia Apoko, Denis Okello, Eddy Walakira, Ruth Kutalek

PLOS Neglected Tropical Diseases: published July 14, 2025 | https://doi.org/10.1371/journal.pntd.0013251

African strains of Zika virus resist ISG-mediated restriction

Inès Bribes, Jim Zoladek, Marion Cannac, Sara Salinas, Sam J. Wilson, Sébastien Nisole

PLOS Neglected Tropical Diseases: published July 14, 2025 | https://doi.org/10.1371/journal.pntd.0013326

The m6A demethylase FTO regulates TNF-α expression in human macrophages following Toxoplasma gondii infection

Min Qin, Nan Gao, Jierui Sun, Shuqing Lin, Tingting Hu, Xinjian Liu, Rong Zhang, Yong Wang, Jingfan Qiu

PLOS Neglected Tropical Diseases: published July 15, 2025 | https://doi.org/10.1371/journal.pntd.0013289

High prevalence of Strongyloides stercoralis in people living with HIV: A critical health challenge in the Peruvian Amazon Basin

Silvia Otero-Rodriguez, Martin Casapia-Morales, Viviana Pinedo-Cancino, Seyer Mego-Campos, Victoria-Ysabel Villacorta-Pezo, Jorge Parráguez-de-la-Cruz, Eva H. Clark, Esperanza Merino, Jose-Manuel Ramos-Rincon

PLOS Neglected Tropical Diseases: published July 15, 2025 | https://doi.org/10.1371/journal.pntd.0013231

Large-scale epidemiology of opisthorchiasis in 21 provinces in Thailand based on diagnosis by fecal egg examination and urine antigen assay and analysis of risk factors for infection

Kulthida Y. Kopolrat, Chanika Worasith, Phattharaphon Wongphutorn, Anchalee Techasen, Chatanun Eamudomkarn, Jiraporn Sithithaworn, Watcharin Loilome, Nisana Namwat, Attapol Titapun, Chaiwat Tawarungruang, Bandit Thinkhamrop, Samarn Futrakul, Simon D. Taylor-Robinson, Melissa R. Haswell, Narong Khuntikeo, Thomas Crellen, Paiboon Sithithaworn

PLOS Neglected Tropical Diseases: published July 16, 2025 | https://doi.org/10.1371/journal.pntd.0013095

Chikungunya in a pediatric cohort: Asymptomatic infection, seroconversion, and chronicity rates

Blenda de Jesus Pereira, Michelle Queiroz Aguiar Brasil, Jessica de Jesus Silva, Juqueline Rocha Cristal, Isabele de Pádua Carvalho, Maria Carolina Prado Miranda, Daniela Paixão, Ricardo Khouri, Thiago Cerqueira-Silva, Fernanda Castro Boulos, Manoel Barral-Netto, Esper Georges Kalas, Aldina Barral, Antônio Bandeira, Viviane Sampaio Boaventura

PLOS Neglected Tropical Diseases: published July 16, 2025 | https://doi.org/10.1371/journal.pntd.0013254

Identifying key aspects to enhance predictive modeling for early identification of schistosomiasis hotspots to guide mass drug administration

Yewen Chen, Fangzhi Luo, Leonardo Martinez, Susan Jiang, Ye Shen

PLOS Neglected Tropical Diseases: published July 16, 2025 | https://doi.org/10.1371/journal.pntd.0013315

Heterologous prime-boost immunization based on a human adenovirus 5 vectored containing Trichinella spiralis Cystatin-like protein elicits protective mucosal immunity in mice

Nuo Xu, Yang Wang, Ning Xu, Zhenhuan Xiang, Dexian Wang, Yao Yu, Mingyuan Liu, Xiaolei Liu, Bin Tang, Xue Bai

PLOS Neglected Tropical Diseases: published July 16, 2025 | https://doi.org/10.1371/journal.pntd.0013323

Genetic shifts of Japanese encephalitis virus (JEV) in mosquitoes in the Republic of Korea, 2017–2022

Bo-Ram Yun, Ji-Young Kwon, Byung-Eon Noh, Sehoon Cho, Dongmi Kwak, Hee Il Lee

PLOS Neglected Tropical Diseases: published July 17, 2025 | https://doi.org/10.1371/journal.pntd.0013258

Contributions of different host species to the natural transmission of severe fever with thrombocytopenia syndrome virus in China

Qu Cheng, Xinqiang Wang, Qi Li, Hailan Yu, Xiaolu Wang, Chenlong Lv, Junhua Tian, Banghua Chen, Zhihang Peng, Liqun Fang, Wei Liu, Yang Yang, Bethan V. Purse

PLOS Neglected Tropical Diseases: published July 17, 2025 | https://doi.org/10.1371/journal.pntd.0013304

Promising therapeutic efficacy of nitazoxanide-loaded zinc oxide nano-formula against intestinal and muscular phases of experimental trichinellosis

Nancy Abd-elkader Hagras, Fatma Hegab, Shimaa Atta, Reham A. Gadallah, Youssef Elsayed, Gehan A. M. Khodear

PLOS Neglected Tropical Diseases: published July 18, 2025 | https://doi.org/10.1371/journal.pntd.0013239

Enhanced rabies surveillance in roadkill specimens by real-time RT-PCR

Crystal M. Gigante, Claire Hartloge, Rene Edgar Condori, Jordona D. Kirby, Lauren Hovis, Kathleen M. Nelson, Ryan Wallace, Yu Li, Richard B. Chipman

PLOS Neglected Tropical Diseases: published July 18, 2025 | https://doi.org/10.1371/journal.pntd.0013348

Cost-effectiveness of adding measurement of Chlamydia trachomatis infection and serology to trachoma prevalence surveys in Tanzania and Mozambique

Emily C. Decker, Molly W. Adams, William E. Oswald, Rebecca M. Flueckiger, Jeremiah M. Ngondi, Mawo Fall, Ezgi E. Yilmaz, Lisa C. Brooks, George Kabona, Henis Mior Sitoe, Mabula Kasubi, Tamimo Momade, Thomas M. Lietman, Thuy Doan, Rachel D. Stelmach

PLOS Neglected Tropical Diseases: published July 21, 2025 | https://doi.org/10.1371/journal.pntd.0013257

Thrombocytopenia in patients with Plasmodium vivax in Colombia is associated with anti-phosphatidylserine autoantibodies and IL-6, IFNγ, IL-10 and TGF-β

María Camila Velasco-Pareja, Catalina Tovar-Acero, Miriam E. Cantero Guevara, Ana Rodriguez, Juan Rivera-Correa, María Fernanda Yasnot-Acosta

PLOS Neglected Tropical Diseases: published July 21, 2025 | https://doi.org/10.1371/journal.pntd.0013284

Fascioliasis in north-central Vietnam: Assessing community knowledge, attitudes, and practices

Vinh Hoang Quang, Bruno Levecke, Dung Do Trung, Binh Vu Thi Lam, Le Thuy Dung, Thuy Duc Nguyen, Tran Thi Tuyen, Hien Thi Thu Nguyen, Nguyen Ngoc Ha, Brecht Devleesschauwer, Kathy Goossens, Theodorus de Jong, Linda Paredis, Nathalie De Wilde, Katja Polman, Steven Callens, Pierre Dorny, Veronique Dermauw

PLOS Neglected Tropical Diseases: published July 21, 2025 | https://doi.org/10.1371/journal.pntd.0013324

Prevalence and risk factors associated with intestinal parasitosis in children with diarrhoea in the Huambo municipality

Ednogildo Domingos Miguel Sachocal, Sandra Cristina Ribeiro Buta da Costa, Hermenegildo Osvaldo Chitumba

PLOS Neglected Tropical Diseases: published July 21, 2025 | https://doi.org/10.1371/journal.pntd.0013314

STAT1-mediated interferon signaling in the hematopoietic system is essential for restricting Usutu virus infection in vivo

Amy N. Nelson, Saloni Sinha, Sydney J. Mullin, Sebastian S. Carver, Thomas R. Cafiero, Aaron E. Lin, Robert E. Schwartz, Alexander Ploss

PLOS Neglected Tropical Diseases: published July 22, 2025 | https://doi.org/10.1371/journal.pntd.0013317

Assessing Aedes mosquito larval indicators, dengue virus infection rates, and risk factors in Khyber Pakhtunkhwa: Insights for improved vector control strategies

Jehangir Khan, Muhammad Adil, Tsheten Tsheten, Pablo Manrique-Saide, Dongjing Zhang, Abdul Aziz, Zhiyue Lv, Tao Chen

PLOS Neglected Tropical Diseases: published July 22, 2025 | https://doi.org/10.1371/journal.pntd.0013252

Outbreak of cutaneous leishmaniasis amongst militia members in a non-endemic district under conflict in the lowlands of Somali Region caused by Leishmania tropica, Eastern Ethiopia

Adugna Abera, Henok Tadesse, Dereje Beyene, Desalegn Geleta, Mahlet Belachew, Ebise Abose Djirata, Solomon Kinde, Hailemariam Difabachew, Tesfahun Bishaw, Mussie Abdosh Hassen, Abdulahi Gire, Tariku Mulatu Bore, Binyam Mohammedbirhan Berhe, Medhanye Habtetsion, Zalalam Olani Tugga, Endawoke Eyelachew, Worku Birhanu Sefer, Kaoutar Choukri, Jasmine Coppens, Gemechu Tadese, Kebron Haile, Henock Bekele, Zeyede Kebede, Gert van der Auwera, Fikre Seife, Melkamu Abte, Getachew Tollera, Mesay Hailu, Jean-Claude Dujardin, Johan van Griensven, Dawit Wolday, Wendemagegn Embiale, Myrthe Pareyn, Geremew Tasew

PLOS Neglected Tropical Diseases: published July 22, 2025 | https://doi.org/10.1371/journal.pntd.0013246

An implementation science framework to understand low coverage in mass dog rabies vaccination

Ricardo Castillo-Neyra, Lizzie Ortiz-Cam, Jorge L. Cañari-Casaño, Elvis W. Díaz, Laura D. Tamayo, Guillermo Porras, Sergio E. Recuenco, Valerie A. Paz-Soldan

PLOS Neglected Tropical Diseases: published July 23, 2025 | https://doi.org/10.1371/journal.pntd.0012869

A community-based vector control intervention "Slash and Clear" implemented in two onchocerciasis-endemic foci in South Sudan

Thomson Luroni Lakwo, Joseph Siewe Fodjo, Stephen Raimon Jada, Peter Alinda, Moses Tionga, Constantino Doggale Remijo Marcello, Deng Gai Dual War, Marina Saleeb, Robert Colebunders

PLOS Neglected Tropical Diseases: published July 23, 2025 | https://doi.org/10.1371/journal.pntd.0013309

Spatial occurrence-intensity modeling of dengue incidence in southernmost provinces of Thailand

Apiradee Lim, Lumpoo Ammatawiyanon, Haris Khurram, Phattrawan Tongkumchum, Don McNeil

PLOS Neglected Tropical Diseases: published July 23, 2025 | https://doi.org/10.1371/journal.pntd.0013347

The long-term persistence of the wMel strain in Rio de Janeiro is threatened by poor integrated vector management and bacterium fitness cost on Aedes aegypti

Márcio Galvão Pavan, Franck Jeannot Gnonhoue, Jessica Corrêa-Antônio, Karine Pedreira Padilha, Gabriela Azambuja Garcia, Felipe de Oliveira, Luiz Paulo Brito, Luciana Dias, Ademir Jesus Martins, Vincent Corbel, José Bento Pereira Lima, Gabriel Luz Wallau, Ary Hoffmann, Oswaldo Gonçalves Cruz, Daniel Antunes Maciel Villela, Rafael Maciel-de-Freitas

PLOS Neglected Tropical Diseases: published July 23, 2025 | https://doi.org/10.1371/journal.pntd.0013372

Association between hydrometeorological conditions and hemorrhagic fever with renal syndrome in Shandong Province, China, from 2005 to 2019

Qi Gao, Chuanlong Cheng, Hui Zuo, Rui Xi, Zhiqiang Wang, Xiujun Li

PLOS Neglected Tropical Diseases: published July 24, 2025 | https://doi.org/10.1371/journal.pntd.0013306

Development and evaluation of a triplex real-time PCR assay for enhanced plague diagnostics in Madagascar

Beza Ramasindrazana, Zoé A. Rahalison, Philippe Gauthier, Guillain Mikaty, Zaina I. Bodoarison, Lanto A. Maminirina, Soloandry Rahajandraibe, Mamy G. Randriamanantsoa, Gilbert Kayoko, Jean-Claude Manuguerra, Anne-Sophie Le Guern, Andriamiliharison J. Rasamindrakotroka, Minoarisoa Rajerison

PLOS Neglected Tropical Diseases: published July 24, 2025 | https://doi.org/10.1371/journal.pntd.0013278

Epidemiological characteristics of human-to-human transmission of severe fever with thrombocytopenia syndrome in China from 1996 to 2023

Li Yuan, Tingting Tian, Aqian Li, Shanshan Du, Shiwen Wang, Dexin Li, Xiaoxia Huang, Jiandong Li

PLOS Neglected Tropical Diseases: published July 24, 2025 | https://doi.org/10.1371/journal.pntd.0013283

Knowledge, attitude, and practice (KAP), and acceptance and willingness to pay (WTP) for mosquito-borne diseases control through sterile mosquito release in Bangkok, Thailand

Pattamaporn Kittayapong, Suwannapa Ninphanomchai, Namon Jalichandra, Luechai Sringernyuang, Penchan Sherer, Natthani Meemon

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0011935

Precision mapping of schistosomiasis and soil-transmitted helminthiasis among school-age children: Targeting interventions in western Kenya

Stella Kepha, Wyckliff Omondi, Maurice R. Odiere, Chitiavi Juma, Martin Muchangi, Dollycate N. Wanja, Chrisistosom Kanyi, Joseph Oloo, Alison Ower, Irene Natalie Chami, Sultani Hadley Matendechero, Florence Wakesho

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0013233

Impact of weather extremes on the spatiotemporal dynamics of visceral leishmaniasis in Brazil

Quinn H. Adams, Emma L. Gause, Rachel E. Baker, Davidson H. Hamer, Guilherme L. Werneck, Lucy R. Hutyra, Kayoko Shioda, Gregory A. Wellenius

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0013316

Noncommunicable diseases associated with bleeding disorders, hospitalization, and mortality in patients with dengue in Mexico: A national analysis of confirmed cases in 2024

Diego Rolando Hernández-Galdamez, Miguel Ángel González-Block, Daniela Karola Romo-Dueñas, Erick Antonio Osorio-López, Rosalba Cerón-Meza, Pablo Méndez-Hernández

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0012933

High seroprevalence of antibodies to Dengue, Chikungunya, and Zika viruses in Dire Dawa, Ethiopia: A cross-sectional survey in 2024

Daniel M. Parker, Werissaw Haileselassie, Temesgen Sisay Hailemariam, Arsema Workenh, Salle Workineh, Xiaoming Wang, Ming-Chieh Lee, Guiyun Yan

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0013357

Epidemiologic, clinical, and therapeutic aspects of formally identified Echis romani bites in northern Cameroon

Jean-Philippe Chippaux, Pierre Amta, Yoann Madec, Rodrigue Ntone, Gaëlle Noël, Pedro Clauteaux, Yap Boum II, Armand S. Nkwescheu, Fabien Taieb

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0013195

Field evaluation of Standard Q Filariasis Antigen Test for Lymphatic Filariasis (LF) during a pre-transmission assessment survey in Sierra Leone, 2022

Benoit Dembele, Mohamed Salieu Bah, Abdulai Conteh, Habib I. Kamara, Ibrahim Kargbo-Labour, Ashley Souza, Patricia Houck, Ernest O. Mensah, Victoria Turay, Eric Scott Elder, Katherine Gass, Steven D. Reid, Joseph P. Shott, Yaobi Zhang, Kimberly Y. Won, Angela Weaver

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0012773

Impact of co-infection with Plasmodium berghei ANKA in Leishmania major-parasitized mice on immune modulation and cutaneous leishmaniasis

Uyla Ornellas-Garcia, Lucas Freire-Antunes, Marcos Rangel-Ferreira, Carina Heusner Gonçalves de Sousa, Mônica Lucas Ribeiro-Almeida, Cláudio Tadeu Daniel-Ribeiro, Patricia Cuervo, Flávia Lima Ribeiro-Gomes

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0013302

Effects of environmental factors on dengue incidence in the Central Region, Burkina Faso: A time series analyses

Jean Claude Romaric Pingdwindé Ouédraogo, Sylvain Ilboudo, Richard Joshua Tetteh, Charles Kyei, Siaka Lougué, Wendlasida Thomas Ouédraogo, Salfo Ouédraogo, David Dosoo, Kwaku Poku Asante, Léon Gueswendé Blaise Savadogo

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0013356

Effectiveness and equity of vaccination strategies against Rift Valley fever in a heterogeneous landscape

Warren S. D. Tennant, Eric Cardinale, Youssouf Moutroifi, Simon E. F. Spencer, Onzade Charafouddine, Michael J. Tildesley, Raphaëlle Métras

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0013346

Field responses of Glossina pallidipes and Glossina fuscipes fuscipes tsetse flies to Novel Repellent Blend and Waterbuck Repellent Compounds in Kenya

Benson M. Wachira, Richard Echodu, Johnson O. Ouma, Imna I. Malele, Daniel Gamba, Michael Okal, Kennedy O. Ogolla, Clement Mangwiro, Robert Opiro, Deusdedit J. Malulu, Bernard Ochieng, Raymond E. Mdachi, Paul O. Mireji

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0013367

Assessing the spatio-temporal risk of Aedes-borne arboviral diseases in non-endemic regions: The case of Northern Spain

Bruno V. Guerrero, Vanessa Steindorf, Rubén Blasco-Aguado, Luís Mateus, Aitor Cevidanes, Jesús F. Barandika, Ana Ramírez de La Peciña Pérez, Joseba Bidaurrazaga Van-Dierdonck, Jesús Angel Ocio Armentia, Nico Stollenwerk, Maíra Aguiar

PLOS Neglected Tropical Diseases: published July 28, 2025 | https://doi.org/10.1371/journal.pntd.0013325

Computational screening of antiviral candidates for Monkeypox virus DNA polymerase and A42R protein

Muhammad Amjid, Muhammad Maroof Khan, Stephen F. Pastore, John B. Vincent, Tahir Muhammad

PLOS Neglected Tropical Diseases: published July 29, 2025 | https://doi.org/10.1371/journal.pntd.0013312

Estimation of Lassa fever incidence rates in West Africa: Development of a modeling framework to inform vaccine trial design

Sean M. Moore, Erica Rapheal, Sandra Mendoza Guerrero, Natalie E. Dean, Steven T. Stoddard

PLOS Neglected Tropical Diseases: published July 29, 2025 | https://doi.org/10.1371/journal.pntd.0012751

A rare case report of neuro-brucellosis with concurrence of depression, visual impairment, bilateral sensorineural hearing loss, and paraplegia

Hossein Pourmontaseri, Maziyar Rismani, Barbad Karami, Zahra Montaseri, Ali Azmi, Sedighe Hooshmandi

PLOS Neglected Tropical Diseases: published July 29, 2025 | https://doi.org/10.1371/journal.pntd.0012824

LeishTec vaccination disrupts vertical transmission of Leishmania infantum

Diogo G. Valadares, Eric Kontowicz, Serena Tang, Angela Toepp, Adam Lima, Mandy Larson, Tara Grinnage-Pulley, Breanna Scorza, Danielle Pessoa-Pereira, Jacob Oleson, Christine Petersen

PLOS Neglected Tropical Diseases: published July 30, 2025 | https://doi.org/10.1371/journal.pntd.0013320

Seasonal change of Burkholderia pseudomallei in paddy field water strongly correlates with ambient temperature: A study in north-central Vietnam

Thi Ngoc Anh Vu, Thi Le Quyen Tran, Nguyen Hai Linh Bui, Trung Thanh Trinh

PLOS Neglected Tropical Diseases: published July 30, 2025 | https://doi.org/10.1371/journal.pntd.0013322

Anti-Cryptosporidium efficacy of BKI-1708, an inhibitor of Cryptosporidium calcium-dependent protein kinase 1

Ryan Choi, Matthew A. Hulverson, Deborah A. Schaefer, Dana P. Betzer, Michael W. Riggs, Wenlin Huang, Vicky Sun, Grant R. Whitman, Molly C. McCloskey, Kennan Marsh, Wayne R. Buck, David S. Wagner, Junhai Yang, Andrew P. Bowman, Rita Ciurlionis, Jubilee Ajiboye, Andrew Hemphill, Dilep K. Sigalapalli, Samuel L.M. Arnold, Lynn K. Barrett, Kayode K. Ojo, Erkang Fan, Wesley C. Van Voorhis

PLOS Neglected Tropical Diseases: published July 30, 2025 | https://doi.org/10.1371/journal.pntd.0013263

Diarrhea in Yemeni children under five: A multi-level analysis of population-based surveys, 1991–2022

Zahir M. Tag, Laith J. Abu-Raddad, Hiam Chemaitelly

PLOS Neglected Tropical Diseases: published July 30, 2025 | https://doi.org/10.1371/journal.pntd.0013297

Outcomes of posterior lamellar tarsal rotation vs bilamellar tarsal rotation for trachomatous trichiasis

John H. Kempen, Yineng Chen, Aida Abashawl, Ahlam Awad Mohammed, Sarity Dodson, Wondu Alemayehu, Alemu Gemechu, Aemero Abateneh Mengesha, Dereje Adugna Kumsa, Tony Succar, Kathleen McWilliams, Fangming Jin, Vatinee Y. Bunya, Maureen G. Maguire, Matthew J. Burton, Gui-shuang Ying, FLuorometholone as Adjunctive MEdical Therapy for Trachomatous Trichiasis Surgery (FLAME) Trial Research Group

PLOS Neglected Tropical Diseases: published July 30, 2025 | https://doi.org/10.1371/journal.pntd.0013152

Water, sanitation, and hygiene (WASH) factors and the incidence of communicable diseases in Urban Bangladesh: Evidence from municipal areas

Mohammad Hayatun Nabi, Fatema Hashem Rupa, Ishrat Jahan, A. B. M. Nahid Hasan, Farah Naz, Iqbal Masud, Mohammad Delwer Hossain Hawlader, Mosharop Hossian

PLOS Neglected Tropical Diseases: published July 31, 2025 | https://doi.org/10.1371/journal.pntd.0013329

A comprehensive murine clinical model for development of countermeasures and studying Mayaro virus infection

Rafael Borges Rosa, Emilene Ferreira de Castro, Débora de Oliveira Santos, Willyenne Marília Dantas, Camila Ayumi Tanaka, Renata Pessôa Germano Mendes, Ronaldo Celerino da Silva, Cláudio Antônio de Moura Pereira, João Paulo Silva Servato, Anaíra Ribeiro Guedes Fonseca Costa, Roberta Vieira de Morais Bronzoni, Lindomar José Pena

PLOS Neglected Tropical Diseases: published July 31, 2025 | https://doi.org/10.1371/journal.pntd.0013333

Unveiling the cardiovascular impact of Zika Virus: Myocardial injury, prothrombotic state, and oxidative stress in immunocompetent mice

Fernanda Marques da Silva, Rafael Aguiar Marschner, Markus Berger, Thais Fumaco Teixeira, Ana Paula Muterle, Letícia Rodrigues, Rafael Teixeira Ribeiro, Paulo Michel Roehe, Diogo Onofre Souza, Carlos Alberto Saraiva Gonçalves, Patrícia Sesterheim

PLOS Neglected Tropical Diseases: published July 31, 2025 | https://doi.org/10.1371/journal.pntd.0013292

Psychological distress, perceived stress, and public stigma in skin neglected tropical diseases in Ghana: A structural equation model of a cross-sectional survey

Samuel Adjorlolo, Stephanopoulos Kofi Junior Osei, Emma Efua Adimado, Mawuko Setordzi, Vincent Valentine Akorli, Lawrencia Obenewaa Aprekua, Paul Adjorlolo

PLOS Neglected Tropical Diseases: published July 31, 2025 | https://doi.org/10.1371/journal.pntd.0013387

MaxEnt modeling and risk evaluation of chagas disease vectors in the domestic cycle of Hidalgo, Mexico

Mónica Chico-Avelino, Josefina Ramos-Frías, Adriana López-Mejía, Santiago Martínez-Calvillo, Rebeca Georgina Manning-Cela

PLOS Neglected Tropical Diseases: published July 31, 2025 | https://doi.org/10.1371/journal.pntd.0013199

Corrections

Correction: A randomised controlled feasibility trial of a BabyWASH household playspace: The CAMPI study

Sophie Budge, Paul Hutchings, Alison Parker, Sean Tyrrel, Sam Norton, Camila Garbutt, Fitsume Woldemedhin, Mohammed Yasin Jemal, Mathewos Moges, Siraj Hussen, Hunachew Beyene

PLOS Neglected Tropical Diseases: published July 24, 2025 | https://doi.org/10.1371/journal.pntd.0013351

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Formal Comments

Response to comment on: [Potential bias in the analysis of prenatal treatment for congenital toxoplasmosis]

Borja Guarch-Ibáñez, Clara Carreras-Abad, Marie Antoinette Frick, Daniel Blázquez-Gamero, Fernando Baquero-Artigao, Isabel Fuentes, Pere Soler-Palacin

PLOS Neglected Tropical Diseases: published July 7, 2025 | https://doi.org/10.1371/journal.pntd.0013138

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  1. Potential bias in the analysis of prenatal treatment for congenital toxoplasmosis

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