November 2012

Cover

Kinshasa, Democratic Republic of the Congo: antibiotic susceptibility test of Salmonella spp. carried out at the bench. Antibiotic disks are placed on an inoculated agar plate; the methanol flame is used to sterilize the tweezers. See Lunguya et al.

Image Credit: Jan Jacobs, Institute of Tropical Medicine

Editorial

Engaging a Rising China through Neglected Tropical Diseases

Peter J. Hotez

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001599

Viewpoints

Yaws Eradication: Facing Old Problems, Raising New Hopes

Andrea Rinaldi

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001837

Considerations in the Design of Clinical Trials to Test Novel Entomological Approaches to Dengue Control

Marcel Wolbers, Immo Kleinschmidt, Cameron P. Simmons, Christl A. Donnelly

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001937

Research Articles

Serum Proteome and Cytokine Analysis in a Longitudinal Cohort of Adults with Primary Dengue Infection Reveals Predictive Markers of DHF

Yadunanda Kumar, Cui Liang, Zheng Bo, Jagath C. Rajapakse, Eng Eong Ooi, Steven R. Tannenbaum

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001887

A New Strategy Based on Smrho Protein Loaded Chitosan Nanoparticles as a Candidate Oral Vaccine against Schistosomiasis

Carolina R. Oliveira, Cíntia M. F. Rezende, Marina R. Silva, Ana Paula Pêgo, Olga Borges, Alfredo M. Goes

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001894

Identification of Compounds with Anti-Proliferative Activity against Trypanosoma brucei brucei Strain 427 by a Whole Cell Viability Based HTS Campaign

Melissa L. Sykes, Jonathan B. Baell, Marcel Kaiser, Eric Chatelain, Sarah R. Moawad, Danny Ganame, Jean-Robert Ioset, Vicky M. Avery

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001896

A Transcriptomic Analysis of Echinococcus granulosus Larval Stages: Implications for Parasite Biology and Host Adaptation

John Parkinson, James D. Wasmuth, Gustavo Salinas, Cristiano V. Bizarro, Chris Sanford, Matthew Berriman, Henrique B. Ferreira, Arnaldo Zaha, Mark L. Blaxter, Rick M. Maizels, Cecilia Fernández

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001897

Correction: A Transcriptomic Analysis of Echinococcus granulosus Larval Stages: Implications for Parasite Biology and Host Adaptation

A Protein Microarray for the Rapid Screening of Patients Suspected of Infection with Various Food-Borne Helminthiases

Jia-Xu Chen, Mu-Xin Chen, Lin Ai, Jun-Hu Chen, Shao-Hong Chen, Yong-Nian Zhang, Yu-Chun Cai, Xing-Quan Zhu, Xiao-Nong Zhou

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001899

Forecast of Dengue Incidence Using Temperature and Rainfall

Yien Ling Hii, Huaiping Zhu, Nawi Ng, Lee Ching Ng, Joacim Rocklöv

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001908

Application of PCR in Serum Samples for Diagnosis of Paracoccidioidomycosis in the Southern Bahia-Brazil

Lucas Dias, Leila Falcão de Carvalho, Carla C. Romano

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001909

Salivary Antigen SP32 Is the Immunodominant Target of the Antibody Response to Phlebotomus papatasi Bites in Humans

Soumaya Marzouki, Maha Abdeladhim, Chaouki Ben Abdessalem, Fabiano Oliveira, Beya Ferjani, Dana Gilmore, Hechmi Louzir, Jesus G. Valenzuela, Mélika Ben Ahmed

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001911

Correction: Salivary Antigen SP32 Is the Immunodominant Target of the Antibody Response to Phlebotomus papatasi Bites in Humans

Transcriptional Profiling of the Bladder in Urogenital Schistosomiasis Reveals Pathways of Inflammatory Fibrosis and Urothelial Compromise

Debalina Ray, Tyrrell A. Nelson, Chi-Ling Fu, Shailja Patel, Diana N. Gong, Justin I. Odegaard, Michael H. Hsieh

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001912

Live Brugia malayi Microfilariae Inhibit Transendothelial Migration of Neutrophils and Monocytes

Jan-Hendrik Schroeder, Bigboy H. Simbi, Louise Ford, Sara R. Cole, Mark J. Taylor, Charlotte Lawson, Rachel A. Lawrence

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001914

The Monetary Burden of Cystic Echinococcosis in Iran

Majid Fasihi Harandi, Christine M. Budke, Sima Rostami

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001915

Molecular Changes in Opisthorchis viverrini (Southeast Asian Liver Fluke) during the Transition from the Juvenile to the Adult Stage

Aaron R. Jex, Neil D. Young, Jittiyawadee Sripa, Ross S. Hall, Jean-Pierre Scheerlinck, Thewarach Laha, Banchob Sripa, Robin B. Gasser

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001916

Modeling Dynamic Introduction of Chikungunya Virus in the United States

Diego Ruiz-Moreno, Irma Sanchez Vargas, Ken E. Olson, Laura C. Harrington

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001918

Human African Trypanosomiasis Diagnosis in First-Line Health Services of Endemic Countries, a Systematic Review

Patrick Mitashi, Epco Hasker, Veerle Lejon, Victor Kande, Jean-Jacques Muyembe, Pascal Lutumba, Marleen Boelaert

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001919

In-Hospital Safety in Field Conditions of Nifurtimox Eflornithine Combination Therapy (NECT) for T. b. gambiense Sleeping Sickness

Caecilia Schmid, Andrea Kuemmerle, Johannes Blum, Salah Ghabri, Victor Kande, Wilfried Mutombo, Medard Ilunga, Ismael Lumpungu, Sylvain Mutanda, Pathou Nganzobo, Digas Tete, Nono Mubwa, Mays Kisala, Severine Blesson, Olaf Valverde Mordt

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001920

Differential Anti-Glycan Antibody Responses in Schistosoma mansoni-Infected Children and Adults Studied by Shotgun Glycan Microarray

Angela van Diepen, Cornelis H. Smit, Loes van Egmond, Narcis B. Kabatereine, Angela Pinot de Moira, David W. Dunne, Cornelis H. Hokke

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001922

Endemic Foci of the Tick-Borne Relapsing Fever Spirochete Borrelia crocidurae in Mali, West Africa, and the Potential for Human Infection

Tom G. Schwan, Jennifer M. Anderson, Job E. Lopez, Robert J. Fischer, Sandra J. Raffel, Brandi N. McCoy, David Safronetz, Nafomon Sogoba, Ousmane Maïga, Sékou F. Traoré

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001924

Corticosteroid-Induced Immunosuppression Ultimately Does Not Compromise the Efficacy of Antibiotherapy in Murine Mycobacterium ulcerans Infection

Teresa G. Martins, Gabriela Trigo, Alexandra G. Fraga, José B. Gama, Adhemar Longatto-Filho, Margarida Saraiva, Manuel T. Silva, António G. Castro, Jorge Pedrosa

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001925

Epidemiological Assessment of Eight Rounds of Mass Drug Administration for Lymphatic Filariasis in India: Implications for Monitoring and Evaluation

Subramanian Swaminathan, Vanamail Perumal, Srividya Adinarayanan, Krishnamoorthy Kaliannagounder, Ravi Rengachari, Jambulingam Purushothaman

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001926

Appraisal of a Leishmania major Strain Stably Expressing mCherry Fluorescent Protein for Both In Vitro and In Vivo Studies of Potential Drugs and Vaccine against Cutaneous Leishmaniasis

Estefania Calvo-Álvarez, Nestor Adrian Guerrero, Raquel Álvarez-Velilla, Christopher Fernández Prada, Jose María Requena, Carmen Punzón, Miguel Ángel Llamas, Francisco J. Arévalo, Luis Rivas, Manuel Fresno, Yolanda Pérez-Pertejo, Rafael Balaña-Fouce, Rosa M. Reguera

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001927

Synchrony of Sylvatic Dengue Isolations: A Multi-Host, Multi-Vector SIR Model of Dengue Virus Transmission in Senegal

Benjamin M. Althouse, Justin Lessler, Amadou A. Sall, Mawlouth Diallo, Kathryn A. Hanley, Douglas M. Watts, Scott C. Weaver, Derek A. T. Cummings

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001928

A Static-Cidal Assay for Trypanosoma brucei to Aid Hit Prioritisation for Progression into Drug Discovery Programmes

Manu De Rycker, Sandra O'Neill, Dhananjay Joshi, Lorna Campbell, David W. Gray, Alan H. Fairlamb

PLOS Neglected Tropical Diseases: published November 29, 2012 | https://doi.org/10.1371/journal.pntd.0001932

Open Release of Male Mosquitoes Infected with a Wolbachia Biopesticide: Field Performance and Infection Containment

Linda O'Connor, Catherine Plichart, Ayo Cheong Sang, Corey L. Brelsfoard, Hervé C. Bossin, Stephen L. Dobson

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001797

Host Life History Strategy, Species Diversity, and Habitat Influence Trypanosoma cruzi Vector Infection in Changing Landscapes

Nicole L. Gottdenker, Luis Fernando Chaves, José E. Calzada, Azael Saldaña, C. Ronald Carroll

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001884

A Novel Procedure for Precise Quantification of Schistosoma japonicum Eggs in Bovine Feces

Bin Xu, Catherine A. Gordon, Wei Hu, Donald P. McManus, Hong-Gen Chen, Darren J. Gray, Chuan Ju, Xiao-Jun Zeng, Geoffrey N. Gobert, Jun Ge, Wei-Ming Lan, Shu-Ying Xie, Wei-Sheng Jiang, Allen G. Ross, Luz P. Acosta, Remigio Olveda, Zheng Feng

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001885

Direct Interaction between EgFABP1, a Fatty Acid Binding Protein from Echinococcus granulosus, and Phospholipid Membranes

Jorge L. Porfido, Gabriela Alvite, Valeria Silva, Malcolm W. Kennedy, Adriana Esteves, Betina Corsico

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001893

Affinity Is an Important Determinant of the Anti-Trypanosome Activity of Nanobodies

Guy Caljon, Benoît Stijlemans, Dirk Saerens, Jan Van Den Abbeele, Serge Muyldermans, Stefan Magez, Patrick De Baetselier

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001902

Correction: Affinity Is an Important Determinant of the Anti-Trypanosome Activity of Nanobodies

Projecting the Long-Term Impact of School- or Community-Based Mass-Treatment Interventions for Control of Schistosoma Infection

Xiaoxia Wang, David Gurarie, Peter L. Mungai, Eric M. Muchiri, Uriel Kitron, Charles H. King

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001903

Development of a Temperature-Switch PCR-Based SNP Typing Method for Mycobacterium ulcerans

Katharina Röltgen, Kobina Assan-Ampah, Emelia Danso, Dorothy Yeboah-Manu, Gerd Pluschke

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001904

First Attempt To Validate Human IgG Antibody Response to Nterm-34kDa Salivary Peptide as Biomarker for Evaluating Exposure to Aedes aegypti Bites

Emmanuel Elanga Ndille, Souleymane Doucoure, Georgia Damien, François Mouchet, Papa Makhtar Drame, Sylvie Cornelie, Herbert Noukpo, Sandra Yamadjako, Armel Djenontin, Nicolas Moiroux, Dorothee Misse, Martin Akogbeto, Vincent Corbel, Marie-Claire Henry, Fabrice Chandre, Thierry Baldet, Franck Remoue

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001905

Population Genetic Structuring in Opisthorchis viverrini over Various Spatial Scales in Thailand and Lao PDR

Nonglak Laoprom, Paiboon Sithithaworn, Ross H. Andrews, Katsuhiko Ando, Thewarach Laha, Sirawut Klinbunga, Joanne P. Webster, Trevor N. Petney

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001906

Correction: Population Genetic Structuring in Opisthorchis viverrini over Various Spatial Scales in Thailand and Lao PDR

Transcriptional Analysis of a Unique Set of Genes Involved in Schistosoma mansoni Female Reproductive Biology

Alexis A. Cogswell, Valerie P. Kommer, David L. Williams

PLOS Neglected Tropical Diseases: published November 15, 2012 | https://doi.org/10.1371/journal.pntd.0001907

A Systematic Review of High Quality Diagnostic Tests for Chagas Disease

Anna M. Afonso, Mark H. Ebell, Rick L. Tarleton

PLOS Neglected Tropical Diseases: published November 8, 2012 | https://doi.org/10.1371/journal.pntd.0001881

Chitinase 3-Like 1 Protein Levels Are Elevated in Schistosoma haematobium Infected Children

Laura J. Appleby, Norman Nausch, Claire D. Bourke, Nadine Rujeni, Nicholas Midzi, Takafira Mduluza, Judith E. Allen, Francisca Mutapi

PLOS Neglected Tropical Diseases: published November 8, 2012 | https://doi.org/10.1371/journal.pntd.0001898

Mechanistic and Single-Dose In Vivo Therapeutic Studies of Cry5B Anthelmintic Action against Hookworms

Yan Hu, Bin Zhan, Brian Keegan, Ying Y. Yiu, Melanie M. Miller, Kathryn Jones, Raffi V. Aroian

PLOS Neglected Tropical Diseases: published November 8, 2012 | https://doi.org/10.1371/journal.pntd.0001900

Urban Cholera Transmission Hotspots and Their Implications for Reactive Vaccination: Evidence from Bissau City, Guinea Bissau

Andrew S. Azman, Francisco J. Luquero, Amabelia Rodrigues, Pedro Pablo Palma, Rebecca F. Grais, Cunhate Na Banga, Bryan T. Grenfell, Justin Lessler

PLOS Neglected Tropical Diseases: published November 8, 2012 | https://doi.org/10.1371/journal.pntd.0001901

Human Beta-Defensin 3 Is Up-Regulated in Cutaneous Leprosy Type 1 Reactions

Anna L. Cogen, Stephen L. Walker, Chrissy H. Roberts, Deanna A. Hagge, Kapil D. Neupane, Saraswoti Khadge, Diana N. J. Lockwood

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001869

Fexinidazole: A Potential New Drug Candidate for Chagas Disease

Maria Terezinha Bahia, Isabel Mayer de Andrade, Tassiane Assíria Fontes Martins, Álvaro Fernando da Silva do Nascimento, Lívia de Figueiredo Diniz, Ivo Santana Caldas, André Talvani, Bernadette Bourdin Trunz, Els Torreele, Isabela Ribeiro

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001870

Burden of Visceral Leishmaniasis in Villages of Eastern Gedaref State, Sudan: An Exhaustive Cross-Sectional Survey

Yolanda Kathrin Mueller, Fabienne Nackers, Khalid A. Ahmed, Marleen Boelaert, Jean-Claude Djoumessi, Rahma Eltigani, Himida Ali Gorashi, Omer Hammam, Koert Ritmeijer, Niven Salih, Dagemlidet Worku, Jean-François Etard, François Chappuis

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001872

CT694 and pgp3 as Serological Tools for Monitoring Trachoma Programs

E. Brook Goodhew, Jeffrey W. Priest, Delynn M. Moss, Guangming Zhong, Beatriz Munoz, Harran Mkocha, Diana L. Martin, Sheila K. West, Charlotte Gaydos, Patrick J. Lammie

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001873

Venezuelan Equine Encephalitis Virus Activity in the Gulf Coast Region of Mexico, 2003–2010

A. Paige Adams, Roberto Navarro-Lopez, Francisco J. Ramirez-Aguilar, Irene Lopez-Gonzalez, Grace Leal, Jose M. Flores-Mayorga, Amelia P. A. Travassos da Rosa, Kali D. Saxton-Shaw, Amber J. Singh, Erin M. Borland, Ann M. Powers, Robert B. Tesh, Scott C. Weaver, Jose G. Estrada-Franco

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001875

Accuracy of a Dual Path Platform (DPP) Assay for the Rapid Point-of-Care Diagnosis of Human Leptospirosis

Scott A. Nabity, Guilherme S. Ribeiro, Carolina Lessa Aquino, Daniele Takahashi, Alcinéia Oliveira Damião, André H. O. Gonçalves, Demócrito B. Miranda-Filho, Rena Greenwald, Javan Esfandiari, Konstantin P. Lyashchenko, Mitermayer G. Reis, Marco A. Medeiros, Albert I. Ko

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001878

Sheep Skin Odor Improves Trap Captures of Mosquito Vectors of Rift Valley Fever

David P. Tchouassi, Rosemary Sang, Catherine L. Sole, Armanda D. S. Bastos, Klaus Mithoefer, Baldwyn Torto

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001879

Relative Neurotoxicity of Ivermectin and Moxidectin in Mdr1ab (−/−) Mice and Effects on Mammalian GABA(A) Channel Activity

Cécile Ménez, Jean-François Sutra, Roger Prichard, Anne Lespine

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001883

Toxocara Seropositivity, Atopy and Wheezing in Children Living in Poor Neighbourhoods in Urban Latin American

Lívia Ribeiro Mendonça, Rafael Valente Veiga, Vitor Camilo Cavalcante Dattoli, Camila Alexandrina Figueiredo, Rosemeire Fiaccone, Jackson Santos, Álvaro Augusto Cruz, Laura Cunha Rodrigues, Philip John Cooper, Lain Carlos Pontes-de-Carvalho, Maurício Lima Barreto, Neuza Maria Alcantara-Neves

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001886

New Insights on Taxonomy, Phylogeny and Population Genetics of Leishmania (Viannia) Parasites Based on Multilocus Sequence Analysis

Mariana C. Boité, Isabel L. Mauricio, Michael A. Miles, Elisa Cupolillo

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001888

Interactions and Potential Implications of Plasmodium falciparum-Hookworm Coinfection in Different Age Groups in South-Central Côte d'Ivoire

Aurélie A. Righetti, Dominik Glinz, Lukas G. Adiossan, Ahou-Yah G. Koua, Sébastien Niamké, Richard F. Hurrell, Rita Wegmüller, Eliézer K. N'Goran, Jürg Utzinger

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001889

Filariasis Attenuates Anemia and Proinflammatory Responses Associated with Clinical Malaria: A Matched Prospective Study in Children and Young Adults

Housseini Dolo, Yaya I. Coulibaly, Benoit Dembele, Siaka Konate, Siaka Y. Coulibaly, Salif S. Doumbia, Abdallah A. Diallo, Lamine Soumaoro, Michel E. Coulibaly, Seidina A. S. Diakite, Aldiouma Guindo, Michael P. Fay, Simon Metenou, Thomas B. Nutman, Amy D. Klion

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001890

Impact of Wolbachia on Infection with Chikungunya and Yellow Fever Viruses in the Mosquito Vector Aedes aegypti

Andrew F. van den Hurk, Sonja Hall-Mendelin, Alyssa T. Pyke, Francesca D. Frentiu, Kate McElroy, Andrew Day, Stephen Higgs, Scott L. O'Neill

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001892

Chrysomya putoria, a Putative Vector of Diarrheal Diseases

Steven W. Lindsay, Thomas C. Lindsay, Jessica Duprez, Martin J. R. Hall, Brenda A. Kwambana, Musa Jawara, Ikumapayi U. Nurudeen, Neneh Sallah, Nigel Wyatt, Umberto D'Alessandro, Margaret Pinder, Martin Antonio

PLOS Neglected Tropical Diseases: published November 1, 2012 | https://doi.org/10.1371/journal.pntd.0001895

Cover

Editorial

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Research Articles

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