Differential expression of Shaw-related K+ channels in the rat central nervous system

@inproceedings{Weiser1994DifferentialEO,
 title={Differential expression of Shaw-related K+ channels in the rat central nervous system},
 author={Michael Weiser and Eleazar Vega-Saenz de Miera and Clifford G. Kentros and Herman Moreno and Linda Franzen and Dean E. Hillman and H. Baker and Bernardo Rudy},
 booktitle={Journal of Neuroscience},
 year={1994},
 url={https://api.semanticscholar.org/CorpusID:15544781}
}
The studies presented here provide important clues for the identification of native homo- and heteromULTimeric ShIII channels in neurons and suggest that heteromultimer formation between ShIII proteins might be a common feature in the CNS.

330 Citations

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98 References

Expression of the mRNAs for the Kv3.1 potassium channel gene in the adult and developing rat brain.
To determine whether these channels are associated with specific types of neurons and whether or not the alternately spliced K+ channel variants are differentially expressed, ribonuclease (RNase) protection assays and in situ hybridization histochemistry were used to localize the specific subsets of neurons containing Kv3.1.1 alpha and Kv 3.1 beta.

Region-specific expression of a K+ channel gene in brain.
Northern blot analysis and in situ hybridization studies reveal the highly localized expression in rat brain of transcripts from a gene (KShIIIA) encoding components for voltage-gated K+ channels, demonstrating the existence of cell-type-specific K+ channel components and suggesting that one reason for the unusually large diversity of K+Channel proteins is the presence of subtypes that participate in specific brain functions.

Characterization of a Shaw‐related potassium channel family in rat brain.
Results suggest that members of the RCK and the Raw potassium channel families express potassium channels which form independent outward current systems, and demonstrates that unrestrained mixing of potassium channel subunits to form hybrid channels does not occur in the rat central nervous system.

K+ current diversity is produced by an extended gene family conserved in Drosophila and mouse.
The Drosophila Shaker gene on the X chromosome has three sister genes, Shal, Shab, and Shaw, which map to the second and third chromosomes, which encodes voltage-gated potassium channels with widely varying kinetics and voltage sensitivity of steady-state inactivation.

Molecular cloning of a member of a third class of Shaker-family K+ channel genes in mammals.
The cloning of RKShIIIA is reported, a cDNA encoding a K+ channel sequence expressed in rat brain that cannot be assigned to either of the two known classes of Sh-family genes in mammals based on sequence analysis.

Evidence for the formation of heteromultimeric potassium channels in Xenopus oocytes
Evidence that the Shaker A-type K+ channels expressed in Xenopus oocytes contain several Shaker polypeptides is provided, and it is suggested that heteromultimer formation may increase K+ channel diversity beyond even the level expected from the large number of K+Channel genes and alternative splicing products.

Distinct spatial and temporal expression patterns of K+ channel mRNAs from different subfamilies
The isolation of two putative K+ channels that define two new subfamilies based upon amino acid sequence similarities with other known K- channels are reported, which are predominantly expressed in the brain and first expressed after about 2 weeks of postnatal cerebellar development.

A family of putative potassium channel genes in Drosophila.
By using a Shaker complementary DNA probe and low-stringency hybridization, three additional family members have now been isolated, Shab, Shaw, and Shal, suggesting the presence of a family of Shaker-like genes in Drosophila.

Heteromultimeric channels formed by rat brain potassium-channel proteins
It is reported here that heteromultimeric K+ channels composed of two different RCK proteins (RCK1 and RCK4) assemble after cotransfection of HeLa cells with the corresponding cDNAs and after coinjection of the respective cRNAs into Xenopus oocytes.
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