Reactome Pathway Database

Schema > Reaction > Entries

nsp14 binds nsp12 Show undefined attributes

authored

[InstanceEdit:9697308] Orlic-Milacic, Marija, 2020年08月12日

category binding

compartment

[Compartment:70101] cytosol

created [InstanceEdit:9694660] Cook, Justin, 2020年07月07日

crossReference

[DatabaseIdentifier:11944893] Mondo:0100096

dbId 9694304

disease

[Disease:9683912] COVID-19

displayName nsp14 binds nsp12

edited

[InstanceEdit:9697657] Orlic-Milacic, Marija, 2020年08月18日

eventOf

[Pathway:R-HSA-9694271] Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) - Homo sapiens

followingEvent

[Reaction:R-HSA-9694524] nsp13 binds nsp12 - Homo sapiens

inferredFrom

[Reaction:R-HSA-9682258] nsp14 binds nsp12 - Homo sapiens

input

isChimeric false

isInDisease true

isInferred true

maxDepth 1

modified [InstanceEdit:9830342] Matthews, Lisa, 2023年03月08日

name

nsp14 binds nsp12

output

[Complex:R-COV-9694597] nsp7:nsp8:nsp12:nsp14:nsp10 [cytosol]

precedingEvent

relatedSpecies

[Species:9681683] Severe acute respiratory syndrome coronavirus 2

releaseDate 2020年09月15日

reviewStatus [ReviewStatus:9821382] five stars

reviewed

[InstanceEdit:9699044] Acencio, Marcio Luis, 2020年09月09日

schemaClass Reaction

species

[Species:48887] Homo sapiens

stId R-HSA-9694304

summation

[Summation:9694297] This COVID-19 event has been created by a combination of computational inference (see https://reactome.org/documentation/inferred-events) from SARS-CoV-1 data and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway.

Non-structural protein 14 (nsp14) of the human SARS coronavirus is a bifunctional enzyme bearing 3'-5 exoribonuclease activity involved in replication fidelity and RNA cap N7-guanine methyltransferase activity involved in 5'-RNA capping. nsp14 binds to the minimal replication and transcription complex (RTC), composed of nsp7, nsp8, and nsp12, by directly binding to nsp12 (the main RNA-dependent RNA polymerase). Binding of nsp14 does not affect the processivity of the RTC (Minskaia et al. 2006, Subissi et al. 2014).

Referrals

(hasEvent)

[Pathway:R-HSA-9694271] Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC)

(precedingEvent)

[Reaction:R-HSA-9694524] nsp13 binds nsp12

(inferredTo)

[Reaction:R-HSA-9682258] nsp14 binds nsp12

© 2025 Reactome

Cite Us!