Fig. 4

Cytotoxicity of the unexpanded and expanded NK cells against tumor cells at different E: T ratios, and 12–16 h coculture in vitro (n = 3). (A) Killing rates of the NK cells against solid and hematological tumor cell lines (including K562, MOLM-13, OCI-AML-3, THP-1, U937, Hep-G2 and OVCAR3). (B) Bar plots summarize the CD107a degranulation by unexpanded NK cells (blue bars), expanded NK cells (red bars) after coculture at a 1:1 E: T ratio with target cells for 5 h (n = 3). Statistical significance is indicated as *p < 0.05, **p < 0.01, ***p < 0.01; bars represent mean values with standard deviation