Ligation, and Transformation iNHIBITORS.
Rich Dudley
rdudley+ at pitt.edu
Tue Mar 9 09:04:30 EST 1999
amherrera at CIGB.EDU.CU wrote:
> Hi all
>> I having a low transformation frequency when transform my
> ligation?
>> I have evidence about the good efficiency of the competent
> cells.
>> The vector I used is 10.kb and the band over 3.3kb
>> Thanking Any Suggestion
>> D.Q.V
How much of your ligation mix are you transforming into what volume of
cells? I do usually heat my reactions 65oc for 10-15 minutes, then ice
cool and spin down the condensate. You may have to transform less of
your reaction mix.
Good luck!
rich
--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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