Ligation, and Transformation iNHIBITORS.
Gerd
gerdn at ibg.uit.no
Tue Mar 9 07:25:25 EST 1999
amherrera at CIGB.EDU.CU wrote:
>> Hi all
>> I having a low transformation frequency when transform my
> ligation?
>> I have evidence about the good efficiency of the competent
> cells.
>> The vector I used is 10.kb and the band over 3.3kb
>> Thanking Any Suggestion
>> D.Q.V
Read somewhere (Invitrogen cloning kit) that 20 min at 65 C to kill
ligase would help transformation efficiency. Do not have any idéa if it
actually works.
Gerd
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