Characterise whole transcriptomes
Oxford Nanopore technology uniquely delivers high outputs of any-length reads that span full-length RNA transcripts, facilitating their accurate, unambiguous analysis and resulting in complete transcriptome characterisation at the isoform level.
Nanopore RNA and cDNA sequencing generates novel insights into differential isoform expression and allele-specific expression, even at single-cell resolution — in one go. Furthermore, with direct RNA sequencing that does not require reverse transcription or amplification, it is possible to detect base modifications (such as methylation) in native RNA transcripts. The scalability of Oxford Nanopore sequencing devices means that researchers do not need to batch a high number of samples, reducing waiting times and generating faster results.
Featured content
Long cDNA sequencing reads enable transcriptome analysis at isoform resolution
In this application note, we compare RNA sequencing methods and demonstrate that sequencing libraries prepared with the updated protocol for the Oxford Nanopore cDNA-PCR Sequencing Kit produce longer reads and improved transcript coverage, and offer benefits over alternative RNA sequencing methods.
Direct RNA Sequencing Kits
In this flyer, learn how to sequence RNA in its native form to detect multiple modifications at single-base resolution, capture full-length transcripts, and quantify gene expression without PCR bias. Plus, discover how the new Direct RNA Barcoding Kit unlocks multiplexed direct RNA sequencing of up to 24 samples in a single run.
Related content
See the whole picture with full-length transcripts
Capture transcriptomic and epitranscriptomic variation, in one go
A guide to transcriptomics with Oxford Nanopore
High-definition transcriptomics
Recommended device for RNA and cDNA sequencing
PromethION 2 Integrated
Offering the flexibility of two independent, high-output PromethION Flow Cells, the compact PromethION 2 device brings the benefits of high-coverage, real-time nanopore sequencing to every lab. Ideal for low-cost access to highly accurate whole transcriptomes.