Icon Gated: Flow Cytometry Analyser
Gated is a free, light-weight and elegant analyser and visualizer of FCS data. It is a cross-platform application with supports of:
- Management of FCS samples into groupings and workspace.
- Spillover compensation
- Hierarchial gating
- Common statistical analysis of sub-populations
- Plotting and visual style customization
- Data normalization, integration and dimensionality reduction built-in
- Layout editor for graphics export
- Automation, macro, and scripting
- Free access to Python backend through scripting engine
- Embedded data package format for reproducible analysis and sharing
See the releases for compiled binary packages for Windows x64 (.Net 10) Download Releases
This is the portable software package that you could decompress anywhere without administrator previlege and run the application. The software will try to update itself if newer versions are released if there are network connection from the GitHub repository releases.
From 0.25.3, the binary package contains an embeddable Python runtime and are capable of executing arbitary code. User should only execute trusted macros and scripts.
Population hierarchy
Layout editor
Data normalization and integration
Data normalization and integration
Scripting
Gated is licensed under GNU GPLv3. This program is distributed in the hope that it will be useful, but without any warranty; without even the implied warranty of merchantability or fitness for a particular purpose. See the GNU General Public License for more details.
Copyright (C) Zheng Yang 2025 - 2026.