🌱 Transcriptome Profiling of Brassica napus Seed Size Development

This repository contains scripts used for transcriptome profiling analyses of two contrasting B. napus genotypes:

• 🟢 Ragged Jack (RJ) – small seed size
• 🟡 Zhongshuang (ZS11) – large seed size

The repository is organized into the following modules:

• From Woolfenden, H. et al. (2024).
  A dataset of tissue-specific gene expression dynamics during seed development in Brassica.
  Figshare. https://doi.org/10.6084/m9.figshare.27854361

🧩 PCA plots for sample quality control.

📊 Identification of differentially expressed genes between genotypes.

🤝 Detection of co-expressed gene clusters.

🧠 Construction and visualization of gene regulatory interactions.

📦 Visualization of seed size distribution across genotypes.

This repository is distributed under the GNU General Public License v3.0.

For questions or collaborations, please contact:
👩‍🔬 Dr. Smita Kurup – smita.kurup@rothamsted.ac.uk

⚠️ Please update working directory paths to match your system before running the scripts.

If you use these scripts, please cite this work using the following Zenodo link: DOI