Talk:Horseradish peroxidase
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Dead link
[edit ]This link is dead: * HRP Reference Centre —Preceding unsigned comment added by 131.174.36.210 (talk) 17:38, 26 November 2010 (UTC) [reply ]
- I've removed the dead link, thanks for pointing that out; if you spot something like this again, feel free to either find a working replacement link, mark it with {{dead link}}, or remove or replace the link as you feel appropriate. 78.105.8.153 (talk) 14:07, 1 June 2012 (UTC) [reply ]
What is substrate?
[edit ]According to Peroxidase substrate only H2O2 This is contradiction and needs editorial interaction. Somogyi26 (talk) 17:07, 22 October 2012 (UTC) [reply ]
Peroxidases have two substrates: one is the oxidant (hydrogen peroxide in this case; it gets reduced to water in the process) and the other is a reductant (most commonly a dye in lab application; it gets oxidized and gives color) which can be one of the many substrates. Catalase is very similar but has one susbtrate: one molecule of hydrogen peroxide is oxidized and the other molecule of hydrogen peroxide is reduced. chami 04:00, 22 November 2013 (UTC) — Preceding unsigned comment added by Ck.mitra (talk • contribs)
Simply wrong...
[edit ]"...applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule".- I know what you are thinking and trying to say but what you have written does not make make sense.
You should write clearly "it is a heme protein that acts on hydrogen peroxide and reduces it to water. It simultaneously oxidizes another molecule which is the second substrate. The..."
What you have written (actually hinted at) is only one application of the enzyme. It has many other uses. In the living system, it acts... — Preceding unsigned comment added by Ck.mitra (talk • contribs) 03:55, 22 November 2013 (UTC) [reply ]