Amido black 10B
From Wikipedia, the free encyclopedia
| Names | |
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| IUPAC name
Sodium 4-amino-5-hydroxy-3-((E)-(4-nitrophenyl)diazenyl)-6-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate
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| Other names
4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt
Amidoschwarz Naphthol blue black Acid Black 1 Acidal Black 10B Acidal Navy Blue 3BR Naphthalene Black 10B Buffalo Black NBR C.I. 20470 | |
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3D model (JSmol)
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| ChEBI |
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| ChemSpider |
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| ECHA InfoCard | 100.012.640 Edit this at Wikidata |
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PubChem CID
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| UNII |
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CompTox Dashboard (EPA)
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| Properties | |
| C22H14N6Na2O9S2 | |
| Molar mass | 616.49 g·mol−1 |
| Appearance | Dark red to black |
| Density | 1.536 g/cm3 |
| ~ 30 g/L at 20 °C (68 °F) | |
| Hazards | |
| GHS labelling: | |
| GHS07: Exclamation mark | |
| Warning | |
| NFPA 704 (fire diamond) |
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Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Chemical compound
Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot.[2] It is also used in criminal investigations to detect blood present with latent fingerprints - it stains the proteins in blood a blue-black color.[3] Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.
See also
[edit ]References
[edit ]- ^ "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017.
- ^ Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. ISBN 978-1-4939-2694-7
- ^ Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. doi:10.1016/j.forsciint.201104006