To install this package, start R and enter:

## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("vsn")

In most cases, you don't need to download the package archive at all.

vsn

This package is for version 2.10 of Bioconductor; for the stable, up-to-date release version, see vsn.

Variance stabilization and calibration for microarray data

Bioconductor version: 2.10

The package implements a method for normalising microarray intensities, both between colours within array, and between arrays. The method uses a robust variant of the maximum-likelihood estimator for the stochastic model of microarray data described in the references (see vignette). The model incorporates data calibration (a.k.a. normalization), a model for the dependence of the variance on the mean intensity, and a variance stabilizing data transformation. Differences between transformed intensities are analogous to "normalized log-ratios". However, in contrast to the latter, their variance is independent of the mean, and they are usually more sensitive and specific in detecting differential transcription.

Author: Wolfgang Huber, with contributions from Anja von Heydebreck. Many comments and suggestions by users are acknowledged, among them Dennis Kostka, David Kreil, Hans-Ulrich Klein, Robert Gentleman, Deepayan Sarkar and Gordon Smyth.

Maintainer: Wolfgang Huber <huber at ebi.ac.uk>

Citation (from within R, enter citation("vsn")):

Installation

To install this package, start R and enter:

## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("vsn")

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("vsn")
PDF Introduction to vsn
PDF Likelihood Calculations for vsn
PDF Verifying and assessing the performance with simulated data
PDF Reference Manual

Details

Version 3.24.0
In Bioconductor since BioC 1.6 (R-2.1) or earlier (> 11 years)
License Artistic-2.0
Depends R (>= 2.10), Biobase(>= 2.5.5)
Imports methods, affy(>= 1.23.4), limma, lattice
LinkingTo
Suggests affydata, hgu95av2cdf
SystemRequirements
Enhances

Package Archives

Follow Installation instructions to use this package in your R session.

Package Source vsn_3.24.0.tar.gz
Windows Binary vsn_3.24.0.zip (32- & 64-bit)
Mac OS X 10.6 (Snow Leopard)
Package Downloads Report Download Stats

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