Professor Akila Mayeda retired and the laboratory was closed on March 31, 2024.
All the genetic information, DNA, is packed in the cell
nucleus-the minimal unit of the life-and believe or not, 99% of the
content is non-coding. The pre-mRNAs transcribed from genes are
indeed horribly interrupted by non-coding sequences, termed
‘introns’. The mRNAs, serving as designs for proteins, are
elaborated after the meticulous work to remove introns, termed
‘splicing’. We, the team of the Division of Gene Expression
Mechanism, has been studying to elucidate the precise mechanism of
this essential splicing,
and trying to investigate the splicing defects causing serious
diseases. We are in the small laboratory, but our teamwork for
17 years has led to considerable accomplishments.
WHAT'S NEW
- 2024年11月15日
- Our paper (Kazuhiro Fukumura et al. ) has been
published in iScience !
Fukumura K., Masuda, A., Takeda, J-i., Nagano, O, Saya, H., Ohno, K., Mayeda, M. (2024). RNPS1 in PSAP complex controls periodic pre-mRNA splicing over the cell cycle. iScience , 27, 111400. - 2023年11月17日
- Our paper (Kazuhiro Fukumura et al.) has been
published in Cell Reports !
Fukumura, K., Sperotto, L., Seuß, S., Kang, H-S., Yoshimoto, R., Sattler, M. & Mayeda, A. (2023). SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns. Cell Rep., 42, 113534. - 2023年9月5日
- Our collaboration paper with Osaka Metropolitan University has
been accepted and published!
Nagamine, H., Yashiro M., Yoshimoto, N., Izumi, M., Sugimoto, A, Nakahama, K., Ogawa, K., Matsumoto, Y., Sawa, K., Tani, Y., Kaneda, H., Mitsuoka, S., Yamada, K., Watanabe, T., Aasai, K., Fukumura, K., Mayeda, A. & Kawaguchi, T. (2023) RBM17 expression is associated with the efficacy of ICI monotherapy in NSCLC with low PD-L1 expression. Anticancer Res. 43,4663–4672. - 2023年6月1日
- Communication article by Dr. Makoto Shimada has been published.
Shimada, M.K. (2023). Splicing modulators are involved in human polyglutamine diversification via protein complexes shuttling between nucleus and cytoplasm. Int. J. Mol. Sci. 24, 9622. - 2022年12月30日
- The preprint of our paper (Kazuhiro Fukumura et al.)
has been posted on the preprint server.
Fukumura, K., Sperotto, L., Seuß, S., Kang, H-S., Yoshimoto, R., Sattler, M. & Mayeda, A. (2022). The RBM17/SPF45–SAP30BP interaction is essential for splicing in a subset of human short introns. bioRxiv doi: 10.1101/2022.12.30.522300v1 - 2022年2月24日
- Our collaboration paper with National Taiwan University Hospital
has been published!
Chua, H.-H, Kameyama, T. and Mayeda, A. & Yeh, T.-H. (2022) Epstein-Barr virus enhances cancer-specific aberrant splicing of TSG101 pre-mRNA. Int. J. Mol. Sci. 23, 2516. - 2021年11月15日
- Taylor & Francis Co. requested our contribution for a
commentary article "Author's Views" and it has been published!
Fukumura, K., Venables, J.P. & Mayeda, A. (2021). SPF45/RBM17-dependent splicing and multidrug resistance to cancer chemotherapy.Mol. Cell. Oncol. 9, in press. - 2021年10月1日
- Assistant Professor, Dr. Kazuhiro Fukumura, has been promoted to
Senior Assistant Professor.
- 2021年8月13日
- Our paper (Kazuhiro Fukumura et al.) has been
published!
Fukumura, K., Yoshimoto, R., Sperotto, L., Kang, H-S., Hirose, T., Inoue, K., Sattler, M. & Mayeda, A. (2021). SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns. Nat. Commun. 12, 4910. - 2021年6月17日
- Our paper by Mr. Yuta Otani (Nippon Shinyaku Co., Ltd.) et
al. has been published!
Otani, Y., Fujita, K.-i., Kameyama, T. and Mayeda, A. (2021) The exon junction complex core represses cancer-specific mature mRNA re-splicing: A potential key role in terminating splicing. Int. J. Mol. Sci. 22, 6519. - 2021年3月29日
- Collaboration paper by Dr. Rei Yoshimoto (former Assistant
Professor) et al. has been published online!
Yoshimoto, R. et al. (2021) Spliceostatin A interaction with SF3B1 limits U1 snRNP availability and causes premature cleavage and polyadenylation. Cell Chem. Biol. 28,1356-1365. - 2020年7月4日
- Our paper by Dr. Rei Yoshimoto et al. has been
published!
Yoshimoto, R., Rahimi, K., Ebbesen, K.K., Hansen, T.B., Kjems, J. and Mayeda, A. (2018) Biosynthesis of circular RNA, ciRS-7/CDR1as is mediated by Mammalian-wide Interspersed Repeats, MIRs.iScience 23,101345. - 2019年2月12日
- Our collaboration paper with National Taiwan University Hospital
has been published online!
Chua, H.-H, Kameyama, T. and Mayeda, A. and Yeh, T.-H. (2019) Cancer-specifically re-spliced TSG101 mRNA promotes invasion and metastasis of nasopharyngeal carcinoma. Int. J. Mol. Sci., 20, 773. - 2018年1月31日
- Our paper by Dr. Kazuhiro Fukumura et al. has been E-published
online!
Fukumura, K., Inoue, K. and Mayeda, A. (2018) Splicing activator RNPS1 suppresses errors in pre-mRNA splicing: A Key factor for mRNA quality control. Biochem. Biophys. Res. Commun., in press. - 2017年3月16日
- Joint Research Project funded by FWF (Austrian Science Fund) and JSPS (Japan Society for the Promotion of Science) was awarded! The title of the research was " Global Profiling of Cancer-Associated Exitrons, Alternatively Spliced Internal Regions of Protein-Coding Exons".
- 2016年10月17日
- Our paper by Dr. Rei Yoshimoto et al. has been E-published
online!
Yoshimoto, R., Kaida, D., Furuno, M., Burroughs, A.M., Noma, S., Suzuki, H., Kawamura, Y., Hayashizaki, Y., Mayeda, A. and Yoshida, M. (2016) Global analysis of pre-mRNA subcellular localization following splicing inhibition by spliceostatin A. RNA, in press. - 2016年10月13日
- Our paper in collaboration with Dr. T. Tsukahara (Japan Advanced
Institute of Science and Technology) has been published online!
Suzuki, H., Aoki, Y., Kameyama, T., Saito, T., Masuda, S., Tanihata, J., Nagata, T., Mayeda, A., Takeda, S. and Tsukahara, T. (2016) Endogenous multiple exon skipping and back-splicing at the DMD mutation hotspot. Int. J. Mol. Sci., 17, 1722. - 2016年8月2日
- Our paper by Dr. Kazuhiro Fukumura et al. has been
E-published online!
Fukumura, K., Wakabayashi, S., Kataoka, N., Sakamoto, H., Suzuki, Y., Nakai, K., Mayeda, A. and Inoue, K. (2016) The exon junaction complex controls the efficient and faithful splicing of a subset of transcripts involved in mitotic cell-cyle progression. Int. J. Mol. Sci., 17, 1153. - 2016年7月15日
- Review (in Japanese) by Drs. Rei Yoshimoto and Akila Mayeda has
been published!
Yoshimoto, R. and Mayeda, A. (2016) circRNA, In Non-coding RNA: Overview of RNA world. Hirose, T. and Tomari, Y., eds. Kagaku-Dojin. pp. 271–279. - 2016年6月15日
- Our paper by Dr. Makoto K. Shimada et al. has been E-published
online!
Shimada, M.K., Sanbonmatsu, R., Yamaguchi-Kabata, Y., Yamasaki, C., Suzuki, Y., Chakraborty, R., Gojobori, T. and Imanishi, T. (2016) Selection pressure on human STR loci and its relevance in repeat expansion disease. Mol. Genet. Genomics., 291, 1851-1869. - 2016年1月5日
- Our paper in collbaboration with Dr. Nobuhiko Emi (Graduate
School of Medicine, Division of Hematology) has been E-published
online!
Abe, A., Mizuta, S., Okamoto, A., Yamamoto, Y., Kameyama, T., Mayeda, A. and Emi, N. (2016) Transcriptional activation of platelet-derived growth factor receptor α and GS homeobox 2 resulting from E26 transformation-specific variant 6 translocation in a case of acute myeloid leukemia with t(4;12)(q12;p13). Int. J. Lab. Hematol., 38, e15–e18. - 2015年11月6日
- Our paper in collbaboration with Dr. Nobuhiko Emi (Graduate
School of Medicine, Division of Hematology) has been E-published
online!
Abe, A., Yamamoto, Y., Iba, S., Kanie, T., Okamoto, A., Tokuda, M., Inaguma, Y., Yanada, M., Morishima, S., Mizuta, S., Akatsuka, Y., Okamoto, M., Kameyama, T., Mayeda, A. and Emi, N. (2015). ETV6-LPXN fusion transcript generated by t(11;12)(q12.1;p13) in a patient with relapsing acute myeloid leukemia with NUP98-HOXA9. Genes Chromosomes Cancer, 55, 242-250. - 2015年10月31日
- Our paper in collbaboration with Dr. Nobuhiko Emi (Graduate
School of Medicine, Division of Hematology) has been E-published
online!
Abe, A., Yamamoto, Y., Iba, S., Okamoto, A., Tokuda, M., Inaguma, Y., Yanada, M., Morishima, S., Kanie, T., Tsuzuki M., Akatsuka, Y., Mizuta, S., Okamoto, M., Kameyama, T., Mayeda, A. and Emi, N. (2015) NUP214-RAC1 and RAC1-COL12A1 fusion in complex variant translocations involving chromosomes 6, 7 and 9 in an acute myeloid leukemia case with DEK-NUP214. Cytogenet. Genome. Res., 146, 279-284. - 2015年10月3日
- Our review by Dr. Rei Yoshimoto et al. has been
E-published online!
Yoshimoto, R., Mayeda, A., Yoshida, M. and Nakagawa, S. (2016) MALAT1 long non-coding RNA in cancer. Biochem. Biophys. Acta, 1859, 192-199. - 2015年5月7日
- Our paper by Dr. Makoto K. Shimada et al. has been
published online!
Shimada, M.K., Sasaki-Haraguchi, N., and Mayeda, A. (2015) Idnetification and validation of evolutionarily conserved unusually short pre-mRNA introns in the human genome. Int. J. Mol. Sci., 16, 10376–10388. - 2015年3月17日
- International research activity of Dr. Toshiki Kameyama (Assistant Professor) has been reported in the WEB site (in Japanese) of Scientific Support Programs for Cancer Research Grant-in-Aid!
- 2015年1月1日
- Our review (in Japanese) by Dr. Toshiki Kameyama (Assistant
Professor) has been published!
Kameyama, T., Mayeda, A. (2015) Exploring the generation of aberrant proteins in cancer cells from 'mRNA re-splicing' event. Pharmacia, 51, 22–26. - 2014年5月22日
- Highlight of our research (in Japanese) has been presented
on-line!
'Searching for fhe mechanism of producing aberrant proteins in cancer cells by mRNA re-splicing events'
Scientific Support Programs for Cancer Research Grant-in-Aid for Scientific Research on Innovative Areas Ministry of Education, Culture, Sports, Science and Technology - 2014年1月11日
- Recent research results by Dr. Makoto Shimada has been reported
in the Japanese newspaper.
'Why human can be survived? Explicate by genome comparison with Neanderthal man '
Chunichi Newspaper(Hardcopy・Web) - 2013年11月11日
- Our paper by Dr. Toshiki Kameyama et al. has been
published!
Matsushita, F., Kameyama, T., Kadokawa, Y. and Marunouchi, T. (2014) Spatio-temporal expression pattern of Myt/NZF family zinc finger transcription factors during mouse nervous system development. Dev. Dyn., 243, 588-600. - 2013年10月14日
- Our paper in collaboration with Dr. Kinji Ohno Laboratory(Nagoya
University Graduate School of Medicine) has been published!
Rahman, M.A., Masuda, A., Ohe, K., Ito, M., Hutchinson, D.O., Mayeda, A., Engel, A.G. and Ohno, K. (2013) HnRNP L and hnRNP LL antagonistically modulate PTB-mediated splicing supression of CHRNA1pre-mRNA. Sci. Rep., 3, 2931. - 2013年1月23日
- Our paper by Dr. Hitoshi Suzuki (former Postdoc; Japan Advanced
Institute of Science and Technology) has been published!
Suzuki, H., Kameyama, T., Ohe, K., Tsukahara, T. and Mayeda, A. (2013) Nested introns in an intron: evidence of multi-stepp splicing of a large intron in the human dystrophin pre-mRNA. FEBS Lett., 587, 555-561. - 2012年6月7日
- The Japanese newspaper has reported the below publication in the
article, entitled
'Aberrant proteins in cancer cells: Finding for the possible mechanism'
Chunichi newspaper - 2012年6月6日
- Our paper by Dr. Toshiki Kameyama (Assistant Professor) has been
published!
Kameyama, T., Suzuki, H. and Mayeda, A. (2012) Re-splicing of mature mRNA in cancer cells promotes activation of distant weak alternative splice sites. Nucleic Acids Res., 40, 7896-7906. - 2012年5月26日
- Our paper by Dr. Noriko Sasaki-Haraguchi (Postdoc) has been
published!
Sasaki-Haraguchi, N., Shimada, M.K., Taniguchi, I., Ohno, M. and Mayeda, A. (2012) Mechanistic insights into human pre-mRNA splicing of human ultra-short introns: Potential unusual mechanism identifies G-rich introns. Biochem. Biophys. Res. Commun., 423, 289–294.