General information of database
info Alternative name Planarian Brain Database
info Creator
Creator Name:
Kiyokazu Agata*
Creator Affiliation:
Kyoto University
Creator Name:
Kaneyasu Nishimura
Creator Affiliation:
Kyoto University
Creator Name:
Osamu Nishimura
Creator Affiliation:
Kyoto University
Creator Name:
Tetsutaro Hayashi
Creator Affiliation:
RIKEN Center for Developmental Biology
Creator Name:
Hiroshi Tarui
Creator Affiliation:
RIKEN Center for Developmental Biology
info Contact address Laboratory for Molecular Developmental Biology, Department of Biophysics, Division of Biological Sciences, Graduate School of Science, Kyoto University
Kitashirakawa-Oiwake, Sakyo, Kyoto 606-8502
Email: planaria-nerves ad
info Database classification Microarray Data and other Gene Expression Databases
info Organism
Taxonomy Name:
Dugesia japonica
info Database description Plabrain DB is a database for planarian nervous system analysis, including results of the gene expression profiling of single cells, the gene expression analysis by whole-mount in situ hybridization and the analysis by Immunohistochemical staining. This is the first case to release the result of gene expression analysis at the single-cell resolution by semi-quantitative single-cell RT-PCR (FBSC-PCR) method using FACS (Fluorescence Activated Cell Sorting) .
info Features and manner of utilization of database Plabrain DB is featured by the unique method which combines the semi-quantitative single-cell RT-PCR with FACS to analyze gene expression in single neurons.
An article "Planaria nervous system" in Scholarpedia also provides information about this database.
Some planarian whole-mount in situ hybridization images related to this database are available at a website of JT Biohitory Research Hall (Japanese version only).
info Background and funding The method of gene expression profiling of single cells was developed by Dr. Kiyokazu Agata at RIKEN Center for Developmental Biology.
Neural-specific gene analysis was supported by a Grant-in-Aid for Creative Scientific Research (Research Project Number: 17GS0318, Project Year: 2005-2009).
info Reference(s)
Article title:
Single-cell gene profiling utilizing FACS and its "index sorting" function for stem cell research
Author name(s):
Tetsutaro Hayashi, Norito Shibata, Ryo Okumura, Tomomi Kudome, Osamu Nishimura, Hiroshi Tarui and Kiyokazu Agata
Journal:
Dev Growth Differ., 52, 131-144 (2010)
Article title:
Analysis of motor function modulated by cholinergic neurons in planarian Dugesia japonica
Author name(s):
Kaneyasu Nishimura, Yoshihisa Kitamura, Takashi Taniguchi and Kiyokazu Agata
Journal:
Neuroscience, 168, 18-30 (2010)
External Links:
Link to PubMed
Link to J-GLOBAL
Article title:
Planarians change their body size by maintaining a constant ratio of different cell types using stem cell system
Author name(s):
Hiroyuki Takeda, Kaneyasu Nishimura and Kiyokazu Agata
Journal:
Zoolog. Sci., 26, 805-813.
External Links:
Link to PubMed
Link to J-GLOBAL
Article title:
Characterization of tyramine beta-hydroxylase in planarian Dugesia japonica: Cloning and expression
Author name(s):
Kaneyasu Nishimura, Yoshihisa Kitamura, Takeshi Inoue, Yoshihiko Umesono, Kanji Yoshimoto, Takashi Taniguchi and Kiyokazu Agata
Journal:
Dev Growth Differ., 52, 131-144 (2010)
External Links:
Link to PubMed
Link to J-GLOBAL
Article title:
Identification and distribution of tryptophan hydroxylase (TPH)-positive neurons in the planarian Dugesia japonica
Author name(s):
Kaneyasu Nishimura, Yoshihisa Kitamura, Takeshi Inoue, Yoshihiko Umesono, Kanji Yoshimoto, Kosei Takeuchi, Takashi Taniguchi and Kiyokazu Agata
Journal:
Neurosci. Res., 59, 101-106 (2007)
External Links:
Link to PubMed
Link to J-GLOBAL
Article title:
Reconstruction of dopaminergic neural network and recovery of behavioral function in planarian regenerates
Author name(s):
Kaneyasu Nishimura, Yoshihisa Kitamura, Takeshi Inoue, Yoshihiko Umesono, Shozo Sano, Kanji Yoshimoto, Masatoshi Inden, Kazuyuki Takata, Takashi Taniguchi, Shun Shimohama and Kiyokazu Agata
Journal:
Dev. Neurobiol., 67, 1059-1078 (2007)
External Links:
Link to PubMed
Link to J-GLOBAL
Original website information
info URL of the original website -
info Operation start date -
info Last update date -
info URL of the portal site -
info Whole data download -
info Referenced database -
info Entry list -
info Query search Not available
info Web services Not available
info URL of Web services -
info Need for user registration -