Figure 5

Increase of microglia/macrophages induced by adoptive transfer of Th1-conditioned cells after SCI. (a) Time course of leukocyte infiltration into the injured spinal cord. Th1-conditioned cells or PBS were injected i.p. on day 4 after SCI. (b) Representative profile of flow cytometric analysis of microglia/macrophages accumulated in the spinal cord on SCI days 6, 10, and 14 (2, 6, and 10 days, respectively, after PBS injection or Th1 cell transfer) of PBS or Th1-conditioned cells. Upper dot plots indicate side-scatter/forward-scatter profile by which CD11b/CD45 profiles were gated. In lower dot plots, CD11b^intermediate CD45^intermediate corresponds to microglia, whereas CD11b^high CD45^high corresponds to macrophages. (c) Quantification of microglia/macrophages accumulated in the injured spinal cord. Leukocytes isolated from the spinal cords of 2–4 mice were analyzed in each experiment. Representative data are shown from 3–4 independent experiments. **P<0.01 versus the control group (Student’s t test)