Figure 2

Transfer of Th1-conditioned cells ameliorates functional recovery after spinal cord injury (SCI). (a) BMS scores are shown for SCI mice after the transfer of 5.0 ×ばつ 10⁶ Th1- or Th2-conditioned cells (PBS, n=3; Th1, n=3; Th2, n=3). (b) Time course of hindlimb locomotion scored by BMS with adoptive transfer of 1.0 ×ばつ 10⁷ each helper T-cell subsets. Transfer of Th1-conditioned cells improved BMS score after SCI (PBS, n=8; Th1, n=7; Th2, n=5; Th17, n=6). (c) Time course of BMS scores after SCI followed by transfer of Th1-conditioned cells stimulated with anti-CD3/anti-CD28 antibodies for 24 h (PBS, n=4; Th1, n=5). (d) Time course of BMS scores for SCI mice after the transfer of anti-IL-2 antibody-treated Th17-conditioned cells (PBS, n=5, Th17+anti-IL-2, n=7). (e) Time course of the inclined plane task. Th1-conditioned cells improved the angle of the inclined plane task (PBS, n=9; Th1, n=6; Th17, n=6). (f) Tactile sensation scored using the von Frey threshold at day 70 after SCI. Th1-conditioned cells improved tactile sensation after SCI. Sham SCI, n=4; PBS, n=5; Th1, n=4; Th17, n=3. Data are mean±S.E.M. *P<0.05, **P<0.01, ***P<0.001; N.S., not significant versus the PBS group (two-way ANOVA with repeated measures, Bonferroni post-test (analysis of motor function-score) or one-way ANOVA with Dunnett post-test (analysis of sensory function-score))