UniProt:Q8NC56 LEMD2

chain
  • initiator methionine:1
  • chain:2-503
checksum 5C24BF18B0423952
comment
  • FUNCTION Nuclear lamina-associated inner nuclear membrane protein that is involved in nuclear structure organization, maintenance of nuclear envelope (NE) integrity and NE reformation after mitosis (PubMed:16339967, PubMed:17097643, PubMed:28242692, PubMed:32494070). Plays a role as transmembrane adapter for the endosomal sorting complexes required for transport (ESCRT), and is thereby involved in ESCRT-mediated NE reformation (PubMed:28242692, PubMed:32494070). Promotes ESCRT-mediated NE closure by recruiting CHMP7 and downstream ESCRT-III proteins IST1/CHMP8 and CHMP2A to the reforming NE during anaphase (PubMed:28242692). During nuclear reassembly, condenses into a liquid-like coating around microtubule spindles and coassembles with CHMP7 to form a macromolecular O-ring seal at the confluence between membranes, chromatin, and the spindle to facilitate early nuclear sealing (PubMed:32494070). Plays a role in the organization of heterochromatin associated with the NE and in the maintenance of NE organization under mechanical stress (By similarity). Required for embryonic development and involved in regulation of several signaling pathways such as MAPK and AKT (By similarity). Required for myoblast differentiation involving regulation of ERK signaling (By similarity). Essential for cardiac homeostasis and proper heart function (By similarity).SUBUNIT Interacts (via N-terminus) with LMNA isoform C (via C-terminus) (in vitro) (PubMed:16339967). Interacts (via LEM domain) with BANF1 (PubMed:32494070). Interacts (via C-terminus) with CHMP7 (PubMed:28242692). Interacts (via N-terminus) with tubulin; the interaction causes microtubule bundling and stabilization (in vitro) (PubMed:32494070).INTERACTION Lamina-associated protein residing in the inner nuclear membrane (INM) of the nuclear envelope (NE) (PubMed:16339967). The localization to the INM is dependent on LMNA (PubMed:16339967). Evenly distributed around the NE during interphase (PubMed:16339967). During metaphase, found in a reticular network (PubMed:28242692). Recruited to the reforming NE on chromatin disks in early anaphase (PubMed:28242692). In late anaphase, concentrates at the NE core proximal to spindle microtubules, and then broadening to a distributed nuclear rim pattern (PubMed:28242692, PubMed:32494070).ALTERNATIVE PRODUCTS Ubiquitously expressed, including bone marrow, brain, kidney, colon, skeletal muscle, thymus, testis and uterus.DOMAIN The LEM domain is required for inner nuclear membrane (INM) localization and contains a BANF1 conserved binding motif which allows localization to chromatin (PubMed:16339967, PubMed:32494070). In late anaphase, as the reforming nuclear envelope (NE) surrounds the chromatin disk, both the LEM domain and the disordered regions are necessary for localization to the NE core (PubMed:32494070).DOMAIN The disordered regions, also named low complexity domain, confer the ability to phase separate (PubMed:32494070). In late anaphase, as the reforming nuclear envelope (NE) surrounds the chromatin disk, both the LEM domain and the disordered regions are necessary for localization to the NE core (PubMed:32494070). During NE reformation, the proline-arginine-rich sequence within the disordered region binds microtubules, targeting LEM2 condensation to spindle microtubules traversing the nascent NE (PubMed:32494070).DOMAIN The winged-helix (WH) region (residues 395-503) activates the ESCRT-II/ESCRT-III hybrid protein CHMP7 to form co-oligomeric rings around spindle microtubules to facilitate early nuclear sealing.PTM Phosphorylated; strongly phosphorylated in mitosis compared to G1/S.DISEASE The disease is caused by variants affecting the gene represented in this entry.DISEASE The disease is caused by variants affecting the gene represented in this entry.
created [InstanceEdit:217385] Schmidt, EE, 2008年03月27日 06:23:53
crossReference
databaseName UniProt
dbId 235011
description
  • recommendedName: LEM domain-containing protein 2 shortName: hLEM2
displayName UniProt:Q8NC56 LEMD2
geneName
  • LEMD2
identifier Q8NC56
isSequenceChanged false
keyword
  • Acetylation
  • Alternative splicing
  • Cataract
  • Cytoplasm
  • Cytoskeleton
  • Disease variant
  • Membrane
  • Nucleus
  • Phosphoprotein
  • Proteomics identification
  • Reference proteome
  • Transmembrane
  • Transmembrane helix
modified [InstanceEdit:9963647] Weiser, Joel, 2025年08月15日
moleculeType Protein
name
  • LEMD2
otherIdentifier
  • 11741576_a_at
  • 11741577_a_at
  • 11743335_s_at
  • 17018309
  • 221496
  • 224980_PM_at
  • 224980_at
  • 2950848
  • 2950849
  • 2950850
  • 2950851
  • 2950852
  • 2950853
  • 2950854
  • 2950855
  • 2950856
  • 2950860
  • 2950861
  • 2950862
  • 2950863
  • 2950864
  • 2950865
  • 2950866
  • 2950867
  • 2950868
  • 2950869
  • 2950870
  • 2950872
  • 2950873
  • 2950874
  • 2950875
  • 2950876
  • 2950877
  • 2950878
  • 2950879
  • 2950880
  • 60869_at
  • 65458_at
  • 8125788
  • A_24_P305960
  • GE850145
  • GE87062
  • GO:0000785
  • GO:0005515
  • GO:0005634
  • GO:0005635
  • GO:0005637
  • GO:0005737
  • GO:0005783
  • GO:0005819
  • GO:0005856
  • GO:0006998
  • GO:0016020
  • GO:0022008
  • GO:0030154
  • GO:0031965
  • GO:0034399
  • GO:0035914
  • GO:0043226
  • GO:0043409
  • GO:0051898
  • GO:0060914
  • GO:0061024
  • GO:0071168
  • GO:0071763
  • HMNXSV003021739
  • Hs.164919.0.S1_3p_at
  • Hs.164919.0.S1_3p_x_at
  • ILMN_1680860
  • PH_hs_0033512
  • TC06001593.hg
physicalEntity
referenceDatabase [ReferenceDatabase:2] UniProt
referenceGene
referenceTranscript
schemaClass ReferenceGeneProduct
secondaryIdentifier
  • LEMD2_HUMAN
  • B4DVH5
  • E7EVT2
  • Q5T972
  • Q5T974
sequenceLength 503
species [Species:48887] Homo sapiens
stId uniprot:Q8NC56
url http://purl.uniprot.org/uniprot/Q8NC56

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