FUNCTION Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2 (PubMed:36402789).FUNCTION Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.CATALYTIC ACTIVITY L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)CATALYTIC ACTIVITY L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)COFACTOR Binds 2 Zn(2+) ions per subunit.SUBUNIT Interacts with TH1L/NELFD.INTERACTION Predominantly in urogenital tissues.PTM Dephosphorylation of Ser-214 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity.MISCELLANEOUS Has a wider tissue distribution than isoform 1, and acts as a dominant-negative antagonist.SIMILARITY Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.