checksum
61EE2173E2351D80
comment
-
FUNCTION Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed:15791618, PubMed:16332456, PubMed:18985798, PubMed:19228692, PubMed:20010382, PubMed:20398791, PubMed:22262466, PubMed:24711118, PubMed:29507376, PubMed:32203132). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (PubMed:16332456). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (PubMed:15901796, PubMed:18245269).CATALYTIC ACTIVITY cholate(in) + ATP + H2O = cholate(out) + ADP + phosphate + H(+)CATALYTIC ACTIVITY taurocholate(in) + ATP + H2O = taurocholate(out) + ADP + phosphate + H(+)CATALYTIC ACTIVITY glycocholate(in) + ATP + H2O = glycocholate(out) + ADP + phosphate + H(+)CATALYTIC ACTIVITY glycochenodeoxycholate(in) + ATP + H2O = glycochenodeoxycholate(out) + ADP + phosphate + H(+)CATALYTIC ACTIVITY taurochenodeoxycholate(in) + ATP + H2O = taurochenodeoxycholate(out) + ADP + phosphate + H(+)CATALYTIC ACTIVITY glycoursodeoxycholate(in) + ATP + H2O = glycoursodeoxycholate(out) + ADP + phosphate + H(+)CATALYTIC ACTIVITY tauroursodeoxycholate(in) + ATP + H2O = tauroursodeoxycholate(out) + ADP + phosphate + H(+)CATALYTIC ACTIVITY taurodeoxycholate(in) + ATP + H2O = taurodeoxycholate(out) + ADP + phosphate + H(+)CATALYTIC ACTIVITY taurolithocholate 3-sulfate(in) + ATP + H2O = taurolithocholate 3-sulfate(out) + ADP + phosphate + H(+)CATALYTIC ACTIVITY pravastatin(in) + ATP + H2O = pravastatin(out) + ADP + phosphate + H(+)ACTIVITY REGULATION The uptake of taurocholate is inhibited by taurolithocholate sulfate with an IC(50) of 9 uM (PubMed:16332456). Pravastatin competitively inhibits the transport of taurocholic acid (PubMed:15901796, PubMed:18985798). Cyclosporin A, glibenclamide, rifampicin and troglitazonestrongly competitively inhibit the transport activity of taurocholate (PubMed:18985798, PubMed:32203132). The canalicular transport activity of taurocholate is strongly dependent on canalicular membrane cholesterol content (PubMed:19228692). The uptake of taurocholate is increased by short- and medium-chain fatty acids (PubMed:20398791). Cholesterol increases transport capacity of taurocholate without affecting the affinity for the substrate (PubMed:24711118).BIOPHYSICOCHEMICAL PROPERTIES Interacts with HAX1 (By similarity). Interacts with the adapter protein complex 2 (AP-2) throught AP2A2 or AP2A1; this interaction regulates cell membrane expression of ABCB11 through its internalization in a clathrin-dependent manner and its subsequent degradation (PubMed:22262466).INTERACTION Internalized at the canalicular membrane through interaction with the adapter protein complex 2 (AP-2) (PubMed:22262466). At steady state, localizes in the canalicular membrane but is also present in recycling endosomes. ABCB11 constantly and rapidly exchanges between the two sites through tubulo-vesicles carriers that move along microtubules. Microtubule-dependent trafficking of ABCB11 is enhanced by taurocholate and cAMP and regulated by STK11 through a PKA-mediated pathway. Trafficking of newly synthesized ABCB11 through endosomal compartment to the bile canalicular membrane is accelerated by cAMP but not by taurocholate (By similarity). Cell membrane expression is up-regulated by short- and medium-chain fatty acids (PubMed:20398791).TISSUE SPECIFICITY Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.DOMAIN Multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.PTM N-glycosylated.PTM Ubiquitinated; short-chain ubiquitination regulates cell-Surface expression of ABCB11.DISEASE The disease is caused by variants affecting the gene represented in this entry.DISEASE The disease is caused by variants affecting the gene represented in this entry.SIMILARITY Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.ONLINE INFORMATION Database for mutations in ABC proteins
databaseName
UniProt
dbId
49402
description
-
recommendedName: fullName evidence="35"Bile salt export pump ecNumber evidence="13 16 27"7.6.2.- alternativeName: ATP-binding cassette sub-family B member 11
displayName
UniProt:O95342 ABCB11
identifier
O95342
isSequenceChanged
false
keyword
-
3D-structure
-
ATP-binding
-
Cell membrane
-
Disease variant
-
Endosome
-
Glycoprotein
-
Intrahepatic cholestasis
-
Lipid transport
-
Membrane
-
Nucleotide-binding
-
Phosphoprotein
-
Proteomics identification
-
Reference proteome
-
Repeat
-
Translocase
-
Transmembrane
-
Transmembrane helix
-
Transport
-
Ubl conjugation
moleculeType
Protein
otherIdentifier
-
11752143_a_at
-
16904793
-
208288_PM_at
-
208288_at
-
211224_PM_s_at
-
211224_s_at
-
2585963
-
2585967
-
2585969
-
2585970
-
2585971
-
2585973
-
2585974
-
2585975
-
2585976
-
2585977
-
2585978
-
2585979
-
2585980
-
2585981
-
2585982
-
2585984
-
2585985
-
2585987
-
2585988
-
2585989
-
2585990
-
2585991
-
2585992
-
2585994
-
2585995
-
2585996
-
2585997
-
2585998
-
2585999
-
2586000
-
2586002
-
2586003
-
2586004
-
2586006
-
2586007
-
2586009
-
2586010
-
2586015
-
31398_at
-
57426_at
-
75945_at
-
76418_at
-
8056583
-
8647
-
A_23_P39703
-
GE54770
-
GE905236
-
GO:0000166
-
GO:0005215
-
GO:0005319
-
GO:0005515
-
GO:0005524
-
GO:0005768
-
GO:0005886
-
GO:0006629
-
GO:0006631
-
GO:0006699
-
GO:0006805
-
GO:0006855
-
GO:0006869
-
GO:0008206
-
GO:0008559
-
GO:0009986
-
GO:0015125
-
GO:0015126
-
GO:0015432
-
GO:0015721
-
GO:0015722
-
GO:0016020
-
GO:0016324
-
GO:0016567
-
GO:0016787
-
GO:0016887
-
GO:0031998
-
GO:0042632
-
GO:0045177
-
GO:0046581
-
GO:0046618
-
GO:0046691
-
GO:0055037
-
GO:0055038
-
GO:0055085
-
GO:0055088
-
GO:0055091
-
GO:0070062
-
GO:0120189
-
GO:0140359
-
GO:1904251
-
HMNXSV003045408
-
HMNXSV003049460
-
ILMN_2232066
-
PH_hs_0009589
-
TC02002508.hg
-
g4502456_3p_at
-
g4704819_3p_a_at
schemaClass
ReferenceGeneProduct
secondaryIdentifier
-
ABCBB_HUMAN
-
Q53TL2
-
Q9UNB2
sequenceLength
1321
stId
uniprot:O95342