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A Novel Antibody for Keratan Sulfate Expressed on Human iPS/ES Cells

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Abstract

Novel marker antibodies to human iPS/ES cells that are capable of distinguishing between human embryonal carcinoma (hEC) cells and human embryonic stem (hES) cells were developed. One of these antibodies, R-10G recognizes a type of keratan sulfate lacking oversulfated structures on hiPS/ES cells. R-10G is different from traditional hiPS/ES marker antibodies, TRA-1-60 and TRA-1-81, in its cellular localization and therefore its epitope structure. Thus, R-10G should be useful not only as a new molecular probe for disclosing the roles of glycans on the surface of hiPS cells in the maintenance of self-renewal and pluripotency and during the process of differentiation but also as a potent tool for the evaluation and standardization of hiPS cells of different tissue origins and histories in regenerative medicine.

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Author information

Authors and Affiliations

  1. Research Center for Glycobiotechnology, Ritsumeikan University, 1-1-1 Nojihigashi, 525-8577, Kusatsu, Shiga, Japan

    Toshisuke Kawasaki, Nobuko Kawasaki & Hiromi Nakao

  2. College of Pharmaceutical Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, 525-8577, Kusatsu, Shiga, Japan

    Hidenao Toyoda

Authors
  1. Toshisuke Kawasaki
  2. Nobuko Kawasaki
  3. Hiromi Nakao
  4. Hidenao Toyoda

Corresponding author

Correspondence to Toshisuke Kawasaki .

Editor information

Editors and Affiliations

  1. Tokyo Metropolitan Institute of Gerontology, Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan

    Tamao Endo

  2. Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany

    Peter H. Seeberger

  3. Dept. Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Gerald W. Hart

  4. Academia Sinica, Nankang, Taipei, Taiwan

    Chi-Huey Wong

  5. Systems Glycobiology Group, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, Wako, Saitama, Japan

    Naoyuki Taniguchi

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Kawasaki, T., Kawasaki, N., Nakao, H., Toyoda, H. (2014). A Novel Antibody for Keratan Sulfate Expressed on Human iPS/ES Cells. In: Endo, T., Seeberger, P., Hart, G., Wong, CH., Taniguchi, N. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54836-2_94-1

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  • DOI: https://doi.org/10.1007/978-4-431-54836-2_94-1

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  • Accepted:

  • Published:

  • Publisher Name: Springer, Tokyo

  • Online ISBN: 978-4-431-54836-2

  • eBook Packages: Living Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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