- Yoshito Ihara 6 ,
- Yoko Inai 6 ,
- Midori Ikezaki 6 ,
- In-Sook L. Matsui 6 ,
- Shino Manabe 7 &
- ...
- Yukishige Ito 7,8
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5100 Accesses
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6 Citations
Abstract
C-Mannosylation is a unique glycosylation in which an α-mannose attaches to the indole C2 carbon atom of a tryptophan (Trp) residue to produce C-mannosyl-tryptophan. C-Mannosylation usually occurs at the first Trp in the consensus amino acid sequence Trp-x-x-Trp (W-x-x-W) in proteins through an enzymatic reaction with a specific mannosyltransferase. Recently, Caenorhabditis elegans DPY-19 was identified as a C-mannosyltransferase. Most substrates for C-mannosylation are part of either the thrombospondin type-1 repeat (TSR) superfamily or the type I cytokine receptor family, suggesting a functional role for C-mannosylation in specific substrate proteins. Although the functions of C-mannosylation have not been fully clarified, site-directed mutagenesis of the C-mannosylation potential site in the W-x-x-W motif has revealed it to be important in the folding or targeting of substrate proteins, such as mucins and ADAMTS-like 1, in the cell. By using chemically synthesized C-mannosylated TSR-derived peptides, it was revealed that C-mannosylated peptides could modulate lipopolysaccharide-induced cellular signaling to produce tumor necrosis factor-α. These accumulated findings indicate that C-mannosylation plays important roles in modulating the functions of acceptor proteins in the cell.
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Editors and Affiliations
Tokyo Metropolitan Institute of Gerontology, Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan
Tamao Endo
Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany
Peter H. Seeberger
Dept. Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Gerald W. Hart
Academia Sinica, Nankang, Taipei, Taiwan
Chi-Huey Wong
Systems Glycobiology Group, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, Wako, Saitama, Japan
Naoyuki Taniguchi
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Ihara, Y., Inai, Y., Ikezaki, M., Matsui, IS.L., Manabe, S., Ito, Y. (2014). C-Mannosylation: A Modification on Tryptophan in Cellular Proteins. In: Endo, T., Seeberger, P., Hart, G., Wong, CH., Taniguchi, N. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54836-2_67-1
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DOI: https://doi.org/10.1007/978-4-431-54836-2_67-1
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Publisher Name: Springer, Tokyo
Online ISBN: 978-4-431-54836-2
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