Virology
Evaluation of the Diasorin Liaison® XL Zika Capture IgM CMIA for Zika virus serological testing☆

https://doi.org/10.1016/j.diagmicrobio.2017年11月01日8 Get rights and content
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Highlights

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    The Liaison® XL Zika Capture IgM assay (LZ-CAM) is compared to Zika IgM ELISAs.
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    LZ-CAM shows lower sensitivity but higher specificity than the recommended ELISA.
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    Most commercial Zika IgM immunoassays show lower sensitivity than LZ-CAM.
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    LZ-CAM greatly reduces laboratory resources and sample turnaround time.

Abstract

Due to the increase of Zika virus (ZIKV) transmission throughout the world, many commercial kits have recently become available to aid in laboratory diagnosis of ZIKV infections in clinical samples. Here, we analyze the fully automated Liaison® XL Zika Capture immunoglobulin M (IgM) assay against the recommended IgM-capture enzyme-linked immunosorbent assay.

Abbreviations

Abs
antibodies
CMIA
chemiluminescent microparticle immunoassay
ELISA
enzyme-linked immunosorbent assay
IgG
immunoglobulin G
IgM
immunoglobulin M
MAC
IgM antibody capture
NS1
nonstructural protein 1
PRNT
plaque reduction neutralization test
PZIP
presumptive Zika IgM positive
PRZN
presumptive recent Zika negative
PRZP
presumptive recent Zika
RT-PCR
real-time polymerase chain reaction
ZIKV
Zika virus

Keywords

Zika
Immunoassay
Diagnostics
Arbovirus
Emerging pathogens

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☆
This work was performed at the National Microbiology Laboratory (1015 Arlington Street, Winnipeg R3E 3R2, Manitoba, Canada) and at Cadman Provincial Laboratory (725 William Avenue, Winnipeg R3C 3Y1, Manitoba, Canada).
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