This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features!
Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

NIH NLM Logo
Log in
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Aug;24(8):1544-1547.
doi: 10.3201/eid2408.180341.

Detection of Dengue Virus among Children with Suspected Malaria, Accra, Ghana

Detection of Dengue Virus among Children with Suspected Malaria, Accra, Ghana

Nicholas Amoako et al. Emerg Infect Dis. 2018 Aug.

Abstract

We report new molecular evidence of locally acquired dengue virus infections in Ghana. We detected dengue viral RNA among children with suspected malaria by using a multipathogen real-time PCR. Subsequent sequence analysis revealed a close relationship with dengue virus serotype 2, which was implicated in a 2016 outbreak in Burkina Faso.

Keywords: Accra; DNV2; Dengue; Ghana; Kintampo; Plasmodium; TaqMan array card; West Africa; assay; children; co-infected; endemic; febrile illness; malaria; malaria parasitemia; multi-pathogen; viruses.

PubMed Disclaimer

Figures

Figure 1
Figure 1
TaqMan array card amplification plots for 2 dengue virus–positive samples and 1 negative sample in study of dengue virus among 166 children with suspected malaria, Accra, Ghana, October 2016–July 2017. Blood samples (2.5 mL of the 5.0 mL collected) obtained from children reporting to the hospital with acute febrile illness (AFI) were screened for 26 pathogens simultaneously by using the real-time PCR TaqMan array card. The cards were in 384-well format, and each well contained 1 μL of reaction mixture (0.75 μL of the extracted total nucleic acid and 0.25 μL of TaqMan Fast Virus 1-step Master Mix; Life Technologies/Applied Biosystems, Foster City, CA, USA). The assays were run on an Applied Biosystems Quant Studio 7 Flex real-time PCR system, according to the manufacturer’s recommendations. Two samples tested positive for dengue virus: A) F227, which amplified with critical Ct of 24.40; and B) F299, which amplified with Ct of 19.35. C) All others tested negative, indicated by amplification signals (ΔRn) below threshold levels at quantification cycle cutoff of 35. Each assay included nucleic acid to serve as amplification controls. External controls were spiked into each to monitor extraction and amplification efficiency, and 1 negative control was included for each batch of extraction to monitor laboratory contamination. Ct, cycle threshold.
Figure 2
Figure 2
Gel electrophoresis of dengue virus–specific RT-PCR products in study of dengue virus among 166 children with suspected malaria, Accra, Ghana, October 2016–July 2017. We completed a conventional RT-PCR assay by using dengue-specific primers from Lanciotti et al. (15) to confirm the results of the TaqMan array card assays. The amplification products (expected size 511 bp) were electrophoresed on 2% agarose gel, stained with ethidium bromide, and viewed under ultraviolet light. Lane 1, molecular weight marker; lanes 2 and 3, test samples; lanes 4 and 5, positive controls; lane 6, negative control; lane 7, empty; lane 8, molecular weight marker. RT-PCR, reverse transcription PCR.

References

    1. Bisoffi Z, Buonfrate D. When fever is not malaria. Lancet Glob Health. 2013;1:e11–2. 10.1016/S2214-109X(13)70013-5 - DOI - PubMed
    1. Malm KL, Bart-Plange C, Armah G, Gyapong J, Adjei SO, Koram K, et al. Malaria as a cause of acute febrile illness in an urban pediatric population in Ghana. Am J Trop Med Hyg. 2012;5(Suppl 1):401. - PMC - PubMed
    1. Isiguzo C, Anyanti J, Ujuju C, Nwokolo E, De La Cruz A, Schatzkin E, et al. Presumptive treatment of malaria from formal and informal drug vendors in Nigeria. PLoS One. 2014;9:e110361. 10.1371/journal.pone.0110361 - DOI - PMC - PubMed
    1. Stoler J, Awandare GA. Febrile illness diagnostics and the malaria-industrial complex: a socio-environmental perspective. BMC Infect Dis. 2016;16:683. 10.1186/s12879-016-2025-x - DOI - PMC - PubMed
    1. Oyero OG, Ayukekbong JA. High dengue NS1 antigenemia in febrile patients in Ibadan, Nigeria. Virus Res. 2014;191:59–61. 10.1016/j.virusres.2014年07月02日3 - DOI - PubMed

Publication types

MeSH terms

LinkOut - more resources

Full text links
CDC-NCEZID full text link CDC-NCEZID Free PMC article
Cite

AltStyle によって変換されたページ (->オリジナル) /