New epidemiological aspects of visceral and cutaneous leishmaniasis in Taza, Morocco

doi:10.1186/s13071-016-1910-x

. 2016 Nov 29;9(1):612.

doi: 10.1186/s13071-016-1910-x.

New epidemiological aspects of visceral and cutaneous leishmaniasis in Taza, Morocco

Maryam Hakkour ^{1

2

3}, Asmae Hmamouch ^{4

5}, Mohamed Mahmoud El Alem ^{4

6}, Abdelkbir Rhalem ⁷, Fatima Amarir ⁸, Mohamed Touzani ⁹, Abderrahim Sadak ⁶, Hajiba Fellah ⁴, Faiza Sebti ⁴

Affiliations

¹ National Reference Laboratory of Leishmaniasis, National Institute of Hygiene, Agdal, Rabat, Morocco. maryam.hakkour@gmail.com.
² Laboratory of Zoology and General Biology, Faculty of Science, Agdal, Rabat, Morocco. maryam.hakkour@gmail.com.
³ Hassan II Institute of Agronomy and Veterinary, Rabat, Morocco. maryam.hakkour@gmail.com.
⁴ National Reference Laboratory of Leishmaniasis, National Institute of Hygiene, Agdal, Rabat, Morocco.
⁵ Laboratory of Microbial Biotechnology, Faculty of Sciences and Techniques, Sidi Mohammed Ben Abdellah University, Fez, Morocco.
⁶ Laboratory of Zoology and General Biology, Faculty of Science, Agdal, Rabat, Morocco.
⁷ Hassan II Institute of Agronomy and Veterinary, Rabat, Morocco.
⁸ Institute of Nursing Professions and Health Techniques, Casablanca, Morocco.
⁹ Delegation of Health, Taza, Morocco.

PMID: 27899126
PMCID: PMC5129210
DOI: 10.1186/s13071-016-1910-x

New epidemiological aspects of visceral and cutaneous leishmaniasis in Taza, Morocco

Maryam Hakkour et al. Parasit Vectors. 2016.

. 2016 Nov 29;9(1):612.

doi: 10.1186/s13071-016-1910-x.

Authors

Maryam Hakkour ^{1

2

3}, Asmae Hmamouch ^{4

5}, Mohamed Mahmoud El Alem ^{4

6}, Abdelkbir Rhalem ⁷, Fatima Amarir ⁸, Mohamed Touzani ⁹, Abderrahim Sadak ⁶, Hajiba Fellah ⁴, Faiza Sebti ⁴

Affiliations

¹ National Reference Laboratory of Leishmaniasis, National Institute of Hygiene, Agdal, Rabat, Morocco. maryam.hakkour@gmail.com.
² Laboratory of Zoology and General Biology, Faculty of Science, Agdal, Rabat, Morocco. maryam.hakkour@gmail.com.
³ Hassan II Institute of Agronomy and Veterinary, Rabat, Morocco. maryam.hakkour@gmail.com.
⁴ National Reference Laboratory of Leishmaniasis, National Institute of Hygiene, Agdal, Rabat, Morocco.
⁵ Laboratory of Microbial Biotechnology, Faculty of Sciences and Techniques, Sidi Mohammed Ben Abdellah University, Fez, Morocco.
⁶ Laboratory of Zoology and General Biology, Faculty of Science, Agdal, Rabat, Morocco.
⁷ Hassan II Institute of Agronomy and Veterinary, Rabat, Morocco.
⁸ Institute of Nursing Professions and Health Techniques, Casablanca, Morocco.
⁹ Delegation of Health, Taza, Morocco.

PMID: 27899126
PMCID: PMC5129210
DOI: 10.1186/s13071-016-1910-x

Abstract

Background: Leishmaniasis is considered among the main endemic diseases in Morocco. However, further knowledge about epidemiological aspects of this disease is needed in several provinces to plan control and preventive strategies to tackle the disease. The present study aims to determine the epidemiological aspect of cutaneous and visceral leishmaniasis in Taza Province from 2007-2014 and to identify the circulating species in this province.

Results: The temporal study from 2007 to 2014 showed that the number of cutaneous leishmaniasis cases increased since 2010. During the period of study, most leishmaniasis cases were detected in both urban and rural areas with 34% of cases detected in two urban localities, Bab Zitouna and Bab tété with 297 and 106 cases, respectively. The molecular study of cutaneous leishmaniasis showed the presence of non-sporadic Leishmania infantum and Leishmania tropica in this province. Regarding visceral leishmaniasis, Leishmania infantum is the species that has been identified.

Conclusions: The epidemio-molecular study of leishmaniasis in Taza Province showed the coexistence of two species of Leishmania in the same foci. They also indicated that CL due to Leishmania infantum is more prevalent than reported in the literature. These results will be helpful for the implementation of control strategies by targeting dogs that constitute a reservoir of Leishmania infantum.

Keywords: ITS1-PCR-RFLP; Leishmania infantum; Leishmania tropica; Leishmaniasis; Morocco; Taza Province.

PubMed Disclaimer

Figures

Fig. 1

Fig. 1

Taza Province on the map and its districts [14]

Fig. 2

Fig. 2

VL and CL cases between 1997 and 2014 in Taza Province

Fig. 3

Fig. 3

Geographical distribution of VL and CL cases in Taza Province (2007–2009)

Fig. 4

Fig. 4

Geographical distribution of VL and CL cases in Taza Province (2010–2012)

Fig. 5

Fig. 5

Geographical distribution of VL and CL cases in Taza Province (2013–2014)

Fig. 6

Fig. 6

Distribution of leishmaniasis cases in Taza’s localities (2007–2014)

Fig. 7

Fig. 7

Average number of leishmaniasis cases in Taza’s localities (2007–2014)

Fig. 8

Fig. 8

Variation of leishmaniasis cases in Taza’s localities in relation to year (2007–2014)

Fig. 9

Fig. 9

Distribution of leishmaniasis cases in relation to age (2007–2014)

Fig. 10

Fig. 10

Application of analysis method ITS1-PCR/RFLP on positive slides of Leishmania in Taza Province. Lanes 1, 2, 5: L. infantum; Lanes 3, 4, 6, 7: L tropica; Lane WM: weight marker 100 bp. Positive controls: Lt, L. tropica; Li, L. infantum; Lm, L. major; C-, negative control;

See this image and copyright information in PMC

References

1. WHO Control of the leishmaniasis . Report of a WHO expert committee. Geneva: WHO Technical Report Series; 2010. p. 793.
1. Rhajaoui M. Les leishmanioses humaines au Maroc: une diversité noso-géographique. Pathol Biol. 2009;10:226–229. - PubMed
1. Remlinger P. Un cas de Kala-azar infantile observé au Maroc. Arch Inst Pasteur Afr Noi. 1921;1:240–1.
1. Rhajaoui M, Abdelmajeed N, Fellah H, Azmi K, Amrir F, Al-jawabreh A, et al. Molecular typing reveals emergence of a new clinic-epidemiologic profile of cutaneous leishmaniasis in Morocco. Emerg Infect Dis. 2007;9:1358–1360. doi: 10.3201/eid1309.060946. - DOI - PMC - PubMed
1. Foley H, Vialatte C, Adde R. Existence dans le sud marocain (Haut Guir) du bouton d’orient à l’état endémique. Bull Soc Pathol Exot. 1914;7:114–115.

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
Other Literature Sources
- scite Smart Citations

[1] WHO Control of the leishmaniasis . Report of a WHO expert committee. Geneva: WHO Technical Report Series; 2010. p. 793.

[2] WHO Control of the leishmaniasis . Report of a WHO expert committee. Geneva: WHO Technical Report Series; 2010. p. 793.

[3] Rhajaoui M. Les leishmanioses humaines au Maroc: une diversité noso-géographique. Pathol Biol. 2009;10:226–229. - PubMed

[4] Rhajaoui M. Les leishmanioses humaines au Maroc: une diversité noso-géographique. Pathol Biol. 2009;10:226–229. - PubMed

[5] Remlinger P. Un cas de Kala-azar infantile observé au Maroc. Arch Inst Pasteur Afr Noi. 1921;1:240–1.

[6] Remlinger P. Un cas de Kala-azar infantile observé au Maroc. Arch Inst Pasteur Afr Noi. 1921;1:240–1.

[7] Rhajaoui M, Abdelmajeed N, Fellah H, Azmi K, Amrir F, Al-jawabreh A, et al. Molecular typing reveals emergence of a new clinic-epidemiologic profile of cutaneous leishmaniasis in Morocco. Emerg Infect Dis. 2007;9:1358–1360. doi: 10.3201/eid1309.060946. - DOI - PMC - PubMed

[8] Rhajaoui M, Abdelmajeed N, Fellah H, Azmi K, Amrir F, Al-jawabreh A, et al. Molecular typing reveals emergence of a new clinic-epidemiologic profile of cutaneous leishmaniasis in Morocco. Emerg Infect Dis. 2007;9:1358–1360. doi: 10.3201/eid1309.060946. - DOI - PMC - PubMed

[9] Foley H, Vialatte C, Adde R. Existence dans le sud marocain (Haut Guir) du bouton d’orient à l’état endémique. Bull Soc Pathol Exot. 1914;7:114–115.

[10] Foley H, Vialatte C, Adde R. Existence dans le sud marocain (Haut Guir) du bouton d’orient à l’état endémique. Bull Soc Pathol Exot. 1914;7:114–115.

Account

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

New epidemiological aspects of visceral and cutaneous leishmaniasis in Taza, Morocco

Affiliations

New epidemiological aspects of visceral and cutaneous leishmaniasis in Taza, Morocco

Authors

Affiliations

Abstract

Figures

References

MeSH terms

LinkOut - more resources

Full Text Sources

Other Literature Sources