Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection
- PMID: 27417494
- DOI: 10.1126/science.aaf8505
Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection
Abstract
Zika virus (ZIKV), a mosquito-borne flavivirus with homology to Dengue virus (DENV), has become a public health emergency. By characterizing memory lymphocytes from ZIKV-infected patients, we dissected ZIKV-specific and DENV-cross-reactive immune responses. Antibodies to nonstructural protein 1 (NS1) were largely ZIKV-specific and were used to develop a serological diagnostic tool. In contrast, antibodies against E protein domain I/II (EDI/II) were cross-reactive and, although poorly neutralizing, potently enhanced ZIKV and DENV infection in vitro and lethally enhanced DENV disease in mice. Memory T cells against NS1 or E proteins were poorly cross-reactive, even in donors preexposed to DENV. The most potent neutralizing antibodies were ZIKV-specific and targeted EDIII or quaternary epitopes on infectious virus. An EDIII-specific antibody protected mice from lethal ZIKV infection, illustrating the potential for antibody-based therapy.
Copyright © 2016, American Association for the Advancement of Science.
Comment in
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VIROLOGY. Diagnostics for Zika virus on the horizon.Speer SD, Pierson TC. Speer SD, et al. Science. 2016 Aug 19;353(6301):750-1. doi: 10.1126/science.aah6187. Science. 2016. PMID: 27540152 No abstract available.
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Immunogenic cross-talk between dengue and Zika viruses.Harrison SC. Harrison SC. Nat Immunol. 2016 Aug 19;17(9):1010-2. doi: 10.1038/ni.3539. Nat Immunol. 2016. PMID: 27540984 No abstract available.
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