Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

doi:10.1186/s13071-015-1044-6

. 2015 Aug 20:8:432.

doi: 10.1186/s13071-015-1044-6.

Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

B L Webster ^{1

2}, M Rabone ³, T Pennance ^{4

5}, A M Emery ⁶, F Allan ⁷, A Gouvras ⁸, S Knopp ^{9

10

11}, A Garba ¹², A A Hamidou ¹³, K A Mohammed ¹⁴, S M Ame ¹⁵, D Rollinson ¹⁶, J P Webster ^{17

18}

Affiliations

¹ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. b.webster@nhm.ac.uk.
² Department of Infectious Disease Epidemiology, Imperial College Faculty of Medicine (St Mary's Campus), Norfolk Place, London, W2 1PG, UK. b.webster@nhm.ac.uk.
³ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. m.rabone@nhm.ac.uk.
⁴ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. t.pennance@nhm.ac.uk.
⁵ RVC Department of Pathology and Pathogen Biology, Centre for Emerging, Endemic and Exotic Diseases (CEEED), Royal Veterinary College, University of London, Hertsfordshire, AL97TA, UK. t.pennance@nhm.ac.uk.
⁶ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. a.emery@nhm.ac.uk.
⁷ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. f.allan@nhm.ac.uk.
⁸ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. a.gouvras@nhm.ac.uk.
⁹ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. s.knopp@unibas.ch.
¹⁰ Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland. s.knopp@unibas.ch.
¹¹ University of Basel, Petersplatz 1, 4003, Basel, Switzerland. s.knopp@unibas.ch.
¹² Réseau International Schistosomoses, Environnement, Aménagement et Lutte (RISEAL-Niger), 333, Avenue des Zarmakoye, B.P. 13724, Niamey, Niger. s.knopp@unibas.ch.
¹³ Réseau International Schistosomoses, Environnement, Aménagement et Lutte (RISEAL-Niger), 333, Avenue des Zarmakoye, B.P. 13724, Niamey, Niger. amina@risealniger.org.
¹⁴ Public Health Laboratory - Ivo de Carneri (PHL-IdC), P.O.BOX 122, Wawi, Chake Chake, Pemba, United Republic of Tanzania. kamsharjyy2@yahoo.com.
¹⁵ Public Health Laboratory - Ivo de Carneri (PHL-IdC), P.O.BOX 122, Wawi, Chake Chake, Pemba, United Republic of Tanzania. shaaliame@yahoo.com.
¹⁶ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. d.rollinson@nhm.ac.uk.
¹⁷ Department of Infectious Disease Epidemiology, Imperial College Faculty of Medicine (St Mary's Campus), Norfolk Place, London, W2 1PG, UK. jowebster@rvc.ac.uk.
¹⁸ RVC Department of Pathology and Pathogen Biology, Centre for Emerging, Endemic and Exotic Diseases (CEEED), Royal Veterinary College, University of London, Hertsfordshire, AL97TA, UK. jowebster@rvc.ac.uk.

PMID: 26329827
PMCID: PMC4557312
DOI: 10.1186/s13071-015-1044-6

Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

B L Webster et al. Parasit Vectors. 2015.

. 2015 Aug 20:8:432.

doi: 10.1186/s13071-015-1044-6.

Authors

B L Webster ^{1

2}, M Rabone ³, T Pennance ^{4

5}, A M Emery ⁶, F Allan ⁷, A Gouvras ⁸, S Knopp ^{9

10

11}, A Garba ¹², A A Hamidou ¹³, K A Mohammed ¹⁴, S M Ame ¹⁵, D Rollinson ¹⁶, J P Webster ^{17

18}

Affiliations

¹ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. b.webster@nhm.ac.uk.
² Department of Infectious Disease Epidemiology, Imperial College Faculty of Medicine (St Mary's Campus), Norfolk Place, London, W2 1PG, UK. b.webster@nhm.ac.uk.
³ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. m.rabone@nhm.ac.uk.
⁴ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. t.pennance@nhm.ac.uk.
⁵ RVC Department of Pathology and Pathogen Biology, Centre for Emerging, Endemic and Exotic Diseases (CEEED), Royal Veterinary College, University of London, Hertsfordshire, AL97TA, UK. t.pennance@nhm.ac.uk.
⁶ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. a.emery@nhm.ac.uk.
⁷ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. f.allan@nhm.ac.uk.
⁸ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. a.gouvras@nhm.ac.uk.
⁹ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. s.knopp@unibas.ch.
¹⁰ Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland. s.knopp@unibas.ch.
¹¹ University of Basel, Petersplatz 1, 4003, Basel, Switzerland. s.knopp@unibas.ch.
¹² Réseau International Schistosomoses, Environnement, Aménagement et Lutte (RISEAL-Niger), 333, Avenue des Zarmakoye, B.P. 13724, Niamey, Niger. s.knopp@unibas.ch.
¹³ Réseau International Schistosomoses, Environnement, Aménagement et Lutte (RISEAL-Niger), 333, Avenue des Zarmakoye, B.P. 13724, Niamey, Niger. amina@risealniger.org.
¹⁴ Public Health Laboratory - Ivo de Carneri (PHL-IdC), P.O.BOX 122, Wawi, Chake Chake, Pemba, United Republic of Tanzania. kamsharjyy2@yahoo.com.
¹⁵ Public Health Laboratory - Ivo de Carneri (PHL-IdC), P.O.BOX 122, Wawi, Chake Chake, Pemba, United Republic of Tanzania. shaaliame@yahoo.com.
¹⁶ Wolfson Wellcome Biomedical Laboratories, Department of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. d.rollinson@nhm.ac.uk.
¹⁷ Department of Infectious Disease Epidemiology, Imperial College Faculty of Medicine (St Mary's Campus), Norfolk Place, London, W2 1PG, UK. jowebster@rvc.ac.uk.
¹⁸ RVC Department of Pathology and Pathogen Biology, Centre for Emerging, Endemic and Exotic Diseases (CEEED), Royal Veterinary College, University of London, Hertsfordshire, AL97TA, UK. jowebster@rvc.ac.uk.

PMID: 26329827
PMCID: PMC4557312
DOI: 10.1186/s13071-015-1044-6

Erratum in

Erratum to: Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium.
Webster BL, Rabone M, Pennance T, Emery AM, Allan F, Gouvras A, Knopp S, Garba A, Hamidou AA, Mohammed KA, Ame SM, Rollinson D, Webster JP. Webster BL, et al. Parasit Vectors. 2015 Oct 9;8:519. doi: 10.1186/s13071-015-1134-5. Parasit Vectors. 2015. PMID: 26453014 Free PMC article.

Abstract

Background: Human urogenital schistosomiasis caused by Schistosoma haematobium is widely distributed across Africa and is increasingly targeted for control and regional elimination. The development of new high-throughput, cost-effective molecular tools and approaches are needed to monitor and evaluate the impact of control programs on the parasite populations. Microsatellite loci are genetic markers that can be used to investigate how parasite populations change over time and in relation to external influences such as control interventions.

Findings: Here, 18 existing S. haematobium microsatellite loci were optimised to enable simultaneous amplification across two novel multiplex microsatellite PCR's, each containing nine loci. Methods were developed for the cost effective and rapid processing and microsatellite analysis of S. haematobium larval stages stored on Whatman-FTA cards and proved robust on miracidia and cercariae collected from Zanzibar and Niger.

Conclusion: The development of these novel and robust multiplex microsatellite assays, in combination with an improved protocol to elute gDNA from Whatman-FTA fixed schistosome larval stages, enables the high-throughput population genetic analysis of S. haematobium. The molecular resources and protocols described here advance the way researchers can perform multi locus-based population genetic analyses of S. haematobium as part of the evaluation and monitoring of schistosomiasis control programmes.

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References

1. World Health Organization. Accelerating work to overcome the global impact of neglected tropical diseases: a roadmap for implementation. 2012. Available: http://www.who.int/neglected_diseases/NTD_RoadMap_2012_Fullversion.pdf
1. Webster JP, Gower CM, Norton AJ. Evolutionary concepts in predicting and evaluating the impact of mass chemotherapy schistosomiasis control programmes on parasites and their hosts. Evol Appl. 2008;1:66–83. doi: 10.1111/j.1752-4571.2007.00012.x. - DOI - PMC - PubMed
1. Glenn TC, Lance SL, McKee AM, McKee AM, Webster BL, Emery AM, et al. Significant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide database. Parasites & Vectors. 2013;6:300. doi: 10.1186/1756-3305年6月30日0. - DOI - PMC - PubMed
1. Gower CM, Gabrielli AF, Sacko M, Dembelé R, Golan R, Emery AM, et al. Population genetics of Schistosoma haematobium: development of novel microsatellite markers and their application to schistosomiasis control in Mali. Parasitology. 2011;138:978–94. doi: 10.1017/S0031182011000722. - DOI - PubMed
1. Emery AM, Allan FE, Rabone ME, Rollinson D. Schistosomiasis collection at NHM (SCAN) Parasites & Vectors. 2012;5:185. doi: 10.1186/1756-3305年5月18日5. - DOI - PMC - PubMed

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[1] World Health Organization. Accelerating work to overcome the global impact of neglected tropical diseases: a roadmap for implementation. 2012. Available: http://www.who.int/neglected_diseases/NTD_RoadMap_2012_Fullversion.pdf

[2] World Health Organization. Accelerating work to overcome the global impact of neglected tropical diseases: a roadmap for implementation. 2012. Available: http://www.who.int/neglected_diseases/NTD_RoadMap_2012_Fullversion.pdf

[3] Webster JP, Gower CM, Norton AJ. Evolutionary concepts in predicting and evaluating the impact of mass chemotherapy schistosomiasis control programmes on parasites and their hosts. Evol Appl. 2008;1:66–83. doi: 10.1111/j.1752-4571.2007.00012.x. - DOI - PMC - PubMed

[4] Webster JP, Gower CM, Norton AJ. Evolutionary concepts in predicting and evaluating the impact of mass chemotherapy schistosomiasis control programmes on parasites and their hosts. Evol Appl. 2008;1:66–83. doi: 10.1111/j.1752-4571.2007.00012.x. - DOI - PMC - PubMed

[5] Glenn TC, Lance SL, McKee AM, McKee AM, Webster BL, Emery AM, et al. Significant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide database. Parasites & Vectors. 2013;6:300. doi: 10.1186/1756-3305年6月30日0. - DOI - PMC - PubMed

[6] Glenn TC, Lance SL, McKee AM, McKee AM, Webster BL, Emery AM, et al. Significant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide database. Parasites & Vectors. 2013;6:300. doi: 10.1186/1756-3305年6月30日0. - DOI - PMC - PubMed

[7] Gower CM, Gabrielli AF, Sacko M, Dembelé R, Golan R, Emery AM, et al. Population genetics of Schistosoma haematobium: development of novel microsatellite markers and their application to schistosomiasis control in Mali. Parasitology. 2011;138:978–94. doi: 10.1017/S0031182011000722. - DOI - PubMed

[8] Gower CM, Gabrielli AF, Sacko M, Dembelé R, Golan R, Emery AM, et al. Population genetics of Schistosoma haematobium: development of novel microsatellite markers and their application to schistosomiasis control in Mali. Parasitology. 2011;138:978–94. doi: 10.1017/S0031182011000722. - DOI - PubMed

[9] Emery AM, Allan FE, Rabone ME, Rollinson D. Schistosomiasis collection at NHM (SCAN) Parasites & Vectors. 2012;5:185. doi: 10.1186/1756-3305年5月18日5. - DOI - PMC - PubMed

[10] Emery AM, Allan FE, Rabone ME, Rollinson D. Schistosomiasis collection at NHM (SCAN) Parasites & Vectors. 2012;5:185. doi: 10.1186/1756-3305年5月18日5. - DOI - PMC - PubMed

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Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

Affiliations

Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium

Authors

Affiliations

Erratum in

Abstract

References

Publication types

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources

Other Literature Sources