Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia

doi:10.1186/s13071-015-0883-5

. 2015 May 13:8:270.

doi: 10.1186/s13071-015-0883-5.

Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia

Araya Gebresilassie ^{1

2}, Ibrahim Abbasi ³, Essayas Aklilu ⁴, Solomon Yared ⁵, Oscar David Kirstein ⁶, Aviad Moncaz ⁷, Habte Tekie ⁸, Meshesha Balkew ⁹, Alon Warburg ¹⁰, Asrat Hailu ¹¹, Teshome Gebre-Michael ¹²

Affiliations

¹ Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia. shambe2006@yahoo.com.
² Department of Biology, College of Natural Science, Jigjiga University, Jigjiga, Ethiopia. shambe2006@yahoo.com.
³ Department of Microbiology and Molecular Genetics, The Institute of Medical Research Israel-Canada The Kuvin Center for the Study of Infectious and Tropical Diseases, Faculty of Medicine, The Hebrew University, Hadassah Medical School, Jerusalem, Israel. Ibrahima@ekmd.huji.ac.il.
⁴ Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia. reyeesayas@gmail.com.
⁵ Department of Biology, College of Natural Science, Jigjiga University, Jigjiga, Ethiopia. solyar2005@yahoo.com.
⁶ Department of Microbiology and Molecular Genetics, The Institute of Medical Research Israel-Canada The Kuvin Center for the Study of Infectious and Tropical Diseases, Faculty of Medicine, The Hebrew University, Hadassah Medical School, Jerusalem, Israel. oscark@ekmd.huji.ac.il.
⁷ Department of Microbiology and Molecular Genetics, The Institute of Medical Research Israel-Canada The Kuvin Center for the Study of Infectious and Tropical Diseases, Faculty of Medicine, The Hebrew University, Hadassah Medical School, Jerusalem, Israel. adi.moncaz@mail.huji.ac.il.
⁸ Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia. habte_tm@yahoo.com.
⁹ Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia. meshesha_b@yahoo.com.
¹⁰ Department of Microbiology and Molecular Genetics, The Institute of Medical Research Israel-Canada The Kuvin Center for the Study of Infectious and Tropical Diseases, Faculty of Medicine, The Hebrew University, Hadassah Medical School, Jerusalem, Israel. alonw@ekmd.huji.ac.il.
¹¹ Department of Microbiology, Immunology and Parasitology, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia. hailu_a2004@yahoo.com.
¹² Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia. teshomegm@gmail.com.

PMID: 25963759
PMCID: PMC4432826
DOI: 10.1186/s13071-015-0883-5

Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia

Araya Gebresilassie et al. Parasit Vectors. 2015.

. 2015 May 13:8:270.

doi: 10.1186/s13071-015-0883-5.

Authors

Araya Gebresilassie ^{1

2}, Ibrahim Abbasi ³, Essayas Aklilu ⁴, Solomon Yared ⁵, Oscar David Kirstein ⁶, Aviad Moncaz ⁷, Habte Tekie ⁸, Meshesha Balkew ⁹, Alon Warburg ¹⁰, Asrat Hailu ¹¹, Teshome Gebre-Michael ¹²

Affiliations

¹ Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia. shambe2006@yahoo.com.
² Department of Biology, College of Natural Science, Jigjiga University, Jigjiga, Ethiopia. shambe2006@yahoo.com.
³ Department of Microbiology and Molecular Genetics, The Institute of Medical Research Israel-Canada The Kuvin Center for the Study of Infectious and Tropical Diseases, Faculty of Medicine, The Hebrew University, Hadassah Medical School, Jerusalem, Israel. Ibrahima@ekmd.huji.ac.il.
⁴ Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia. reyeesayas@gmail.com.
⁵ Department of Biology, College of Natural Science, Jigjiga University, Jigjiga, Ethiopia. solyar2005@yahoo.com.
⁶ Department of Microbiology and Molecular Genetics, The Institute of Medical Research Israel-Canada The Kuvin Center for the Study of Infectious and Tropical Diseases, Faculty of Medicine, The Hebrew University, Hadassah Medical School, Jerusalem, Israel. oscark@ekmd.huji.ac.il.
⁷ Department of Microbiology and Molecular Genetics, The Institute of Medical Research Israel-Canada The Kuvin Center for the Study of Infectious and Tropical Diseases, Faculty of Medicine, The Hebrew University, Hadassah Medical School, Jerusalem, Israel. adi.moncaz@mail.huji.ac.il.
⁸ Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia. habte_tm@yahoo.com.
⁹ Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia. meshesha_b@yahoo.com.
¹⁰ Department of Microbiology and Molecular Genetics, The Institute of Medical Research Israel-Canada The Kuvin Center for the Study of Infectious and Tropical Diseases, Faculty of Medicine, The Hebrew University, Hadassah Medical School, Jerusalem, Israel. alonw@ekmd.huji.ac.il.
¹¹ Department of Microbiology, Immunology and Parasitology, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia. hailu_a2004@yahoo.com.
¹² Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia. teshomegm@gmail.com.

PMID: 25963759
PMCID: PMC4432826
DOI: 10.1186/s13071-015-0883-5

Abstract

Background: Blood-feeding behavior studies are important for estimating the efficiency of pathogen transmission and assessing the relative human disease risk. However, in Ethiopia and other parts of East Africa there are large remaining gaps in identifying the feeding habits of Phlebotomus orientalis, the vector of Leishmania donovani. The aim of the study was to determine the blood feeding patterns of P. orientalis in Tahtay Adiyabo district, northern Ethiopia.

Methods: For bloodmeal analysis, sandflies were collected from three different villages of Tahtay Adiyabo district using CDC light traps, sticky traps, and pyrethrum spray catches. Bloodmeal of engorged female sandflies was identified using cytochrome (cyt) b-PCR and reverse-line blotting (RLB) and enzyme linked immunosorbent assay (ELISA) assays.

Results: Most (637/641) of the females analyzed were P. orientalis. Successful identification of the host from bloodmeals was achieved in 83.03 and 92.1% using cyt b PCR-RLB and ELISA, respectively. Bloodmeal analysis of P. orientalis females revealed that they have a range of hosts with predominant preference to bovines followed by donkey, human, goat, sheep, dog, and camel.

Conclusion: Results obtained from bloodmeal analyses demonstrate that the feeding preference of P. orientalis is mainly zoophilic, which could vary depending on the availability of hosts.

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Figures

Fig. 1

Fig. 1

Representative RLB results of Cyt b PCR products from blood-fed, P. orientalis. Sample number 1: human blood. Sample 2: cow blood. Sample 3: goat blood. Sample 4: cow and goat. Sample 5: human and cow blood. +ve sample is cow blood. -ve sample with no PCR product

See this image and copyright information in PMC

References

1. Desjeux P. Leishmaniasis: current situation and new perspectives. Comp Imunnol Microbiol Infect Dis. 2004;27:305–18. doi: 10.1016/j.cimid.200403004. - DOI - PubMed
1. Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, et al. Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 2012;7 doi: 10.1371/journal.pone.0035671. - DOI - PMC - PubMed
1. Ali A, Ashford RW. Visceral leishmaniasis in Ethiopia. IV. Prevalence, incidence and relation of infection to disease in an endemic area. Ann. Trop. Med. Parasitol. 1994;88:289–93. - PubMed
1. Lyons S, Veeken H, Long J. Visceral leishmaniasis and HIV in Tigray, Ethiopia. Trop. Med. Int. Health. 2003;8:733–9. doi: 10.1046/j.1365-3156.2003.01088.x. - DOI - PubMed
1. Hailu A, Gebre-Michael T, Berhe N, Balkew M. Leishmaniasis in Ethiopia. In: Berhane Y, Haile-Mariam D, Kloos H, editors. The Ecology and Epidemiology of Health and Disease in Ethiopia. 1. Addis Ababa: Shama Books; 2006. pp. 615–34.

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[1] Desjeux P. Leishmaniasis: current situation and new perspectives. Comp Imunnol Microbiol Infect Dis. 2004;27:305–18. doi: 10.1016/j.cimid.200403004. - DOI - PubMed

[2] Desjeux P. Leishmaniasis: current situation and new perspectives. Comp Imunnol Microbiol Infect Dis. 2004;27:305–18. doi: 10.1016/j.cimid.200403004. - DOI - PubMed

[3] Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, et al. Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 2012;7 doi: 10.1371/journal.pone.0035671. - DOI - PMC - PubMed

[4] Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, et al. Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 2012;7 doi: 10.1371/journal.pone.0035671. - DOI - PMC - PubMed

[5] Ali A, Ashford RW. Visceral leishmaniasis in Ethiopia. IV. Prevalence, incidence and relation of infection to disease in an endemic area. Ann. Trop. Med. Parasitol. 1994;88:289–93. - PubMed

[6] Ali A, Ashford RW. Visceral leishmaniasis in Ethiopia. IV. Prevalence, incidence and relation of infection to disease in an endemic area. Ann. Trop. Med. Parasitol. 1994;88:289–93. - PubMed

[7] Lyons S, Veeken H, Long J. Visceral leishmaniasis and HIV in Tigray, Ethiopia. Trop. Med. Int. Health. 2003;8:733–9. doi: 10.1046/j.1365-3156.2003.01088.x. - DOI - PubMed

[8] Lyons S, Veeken H, Long J. Visceral leishmaniasis and HIV in Tigray, Ethiopia. Trop. Med. Int. Health. 2003;8:733–9. doi: 10.1046/j.1365-3156.2003.01088.x. - DOI - PubMed

[9] Hailu A, Gebre-Michael T, Berhe N, Balkew M. Leishmaniasis in Ethiopia. In: Berhane Y, Haile-Mariam D, Kloos H, editors. The Ecology and Epidemiology of Health and Disease in Ethiopia. 1. Addis Ababa: Shama Books; 2006. pp. 615–34.

[10] Hailu A, Gebre-Michael T, Berhe N, Balkew M. Leishmaniasis in Ethiopia. In: Berhane Y, Haile-Mariam D, Kloos H, editors. The Ecology and Epidemiology of Health and Disease in Ethiopia. 1. Addis Ababa: Shama Books; 2006. pp. 615–34.

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Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia

Affiliations

Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia

Authors

Affiliations

Abstract

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References

Publication types

MeSH terms

LinkOut - more resources

Full Text Sources

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