This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features!
Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

NIH NLM Logo
Log in
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Jan;92(1):201-9.
doi: 10.4269/ajtmh.14-0277. Epub 2014 Nov 4.

Local evolution of pyrethroid resistance offsets gene flow among Aedes aegypti collections in Yucatan State, Mexico

Affiliations

Local evolution of pyrethroid resistance offsets gene flow among Aedes aegypti collections in Yucatan State, Mexico

Karla Saavedra-Rodriguez et al. Am J Trop Med Hyg. 2015 Jan.

Abstract

The mosquito Aedes aegypti is the major vector of the four serotypes of dengue virus (DENV1-4). Previous studies have shown that Ae. aegypti in Mexico have a high effective migration rate and that gene flow occurs among populations that are up to 150 km apart. Since 2000, pyrethroids have been widely used for suppression of Ae. aegypti in cities in Mexico. In Yucatan State in particular, pyrethroids have been applied in and around dengue case households creating an opportunity for local selection and evolution of resistance. Herein, we test for evidence of local adaptation by comparing patterns of variation among 27 Ae. aegypti collections at 13 single nucleotide polymorphisms (SNPs): two in the voltage-gated sodium channel gene para known to confer knockdown resistance, three in detoxification genes previously associated with pyrethroid resistance, and eight in putatively neutral loci. The SNPs in para varied greatly in frequency among collections, whereas SNPs at the remaining 11 loci showed little variation supporting previous evidence for extensive local gene flow. Among Ae. aegypti in Yucatan State, Mexico, local adaptation to pyrethroids appears to offset the homogenizing effects of gene flow.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Map of collection sites across the Yucatan State.
Figure 2.
Figure 2.
Analysis of the proportion of significant linkage disequilibrium (N = 78 pairwise comparisons) for each community and collection site. Bars represent Bayesian 95% highest density intervals (95% HDI). Proportions with HDI that overlap 0.05 were not considered to have a credible excess of linkage disequilibrium.
Figure 3.
Figure 3.
Regression analysis of allele frequencies of two para mutations: I1,016 and C1,534 in 27 collections from Yucatan.
Figure 4.
Figure 4.
(A) Regression analysis of pairwise FST/(1-FST) for all 13 loci against ln(geographic distances[km]). (B) pairwise FST/(1-FST) for the putatively neutral loci against ln(geographic distances [km]), and (C) pairwise FST/(1-FST) for insecticide resistance loci against ln(geographic distances [km]). Regression analysis equation and correlation coefficient are also shown.

References

    1. Gubler DJ. The changing epidemiology of yellow fever and dengue, 1900 to 2003: full circle? Comp Immunol Microbiol Infect Dis. 2004;27:319–330. - PubMed
    1. García-Rejón JE, López-Uribe MP, Loroño-Pino MA, Arana-Guardia R, Puc-Tinal M, López-Uribe GM, Coba-Tún C, Baak-Baak CM, Machain-Williams C, Reyes-Solis GC, Lozano-Fuentes S, Saavedra-Rodriguez K, Black WC, Beaty BJ, Eisen L. Aedes (Stegomyia) aegypti and Aedes (Howardina) cozumelensis in Yucatán State, México, with a summary of published collection records for Ae. cozumelensis. J Vector Ecol. 2012;37:365–372. - PMC - PubMed
    1. Loroño-Pino MA, Farfán-Ale JA, Zapata-Peraza AL, Rosado-Paredes EP, Flores-Flores LF, García-Rejón JE, Díaz FJ, Blitvich BJ, Andrade-Narváez M, Jiménez-Ríos E, Blair CD, Olson KE, Black W, 4th, Beaty BJ. Introduction of the American/Asian genotype of dengue 2 virus into the Yucatan State of Mexico. Am J Trop Med Hyg. 2004;71:485–492. - PubMed
    1. Loroño-Pino MA, García-Rejón JE, Machain-Williams C, Gomez-Carro S, Nuñez-Ayala G, Nájera-Vázquez Mdel R, Losoya A, Aguilar L, Saavedra-Rodriguez K, Lozano-Fuentes S, Beaty MK, Black WC, 4th, Keefe TJ, Eisen L, Beaty BJ. Towards a Casa Segura: a consumer product study of the effect of insecticide-treated curtains on Aedes aegypti and dengue virus infections in the home. Am J Trop Med Hyg. 2013;89:385–397. - PMC - PubMed
    1. World Health Organization . Dengue and Dengue Hemorrhagic Fever. 2009. 117 WFSN.

Publication types

LinkOut - more resources

Full text links
Sheridan PubFactory full text link Sheridan PubFactory Free PMC article
Cite

AltStyle によって変換されたページ (->オリジナル) /