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. 2014 Sep;80(1):40-2.
doi: 10.1016/j.diagmicrobio.2014年01月03日1. Epub 2014 Mar 7.

Parasite load estimation by qPCR differentiates between asymptomatic and symptomatic infection in Indian visceral leishmaniasis

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Parasite load estimation by qPCR differentiates between asymptomatic and symptomatic infection in Indian visceral leishmaniasis

Medhavi Sudarshan et al. Diagn Microbiol Infect Dis. 2014 Sep.

Abstract

Using quantitative PCR (qPCR), we differentiated asymptomatic and symptomatic Indian Leishmania donovani infection. qPCR on blood of 40 visceral leishmaniasis, 130 endemic, and 40 non-endemic healthy controls showed 500 times less (P < .0001) parasitemia in asymptomatic compared to the symptomatic ones and threshold of 5 parasite genome/mL for the clinical disease.

Keywords: Asymptomatic; Visceral leishmaniasis; patients; qPCR.

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Figures

Figure. 1
Figure. 1
Standard curve generated by different primers.
Figure. 2
Figure. 2
Melt curve analysis for presence or absence of Leishmania parasite.
Figure. 3
Figure. 3
Parasitemia level in active cases and EHC(asymptomatics).

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