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. 2014 Apr;90(4):638-45.
doi: 10.4269/ajtmh.13-0292. Epub 2014 Feb 10.

Development of a specimen-sparing multichannel bead assay to detect antiparasite IgG4 for the diagnosis of Schistosoma and Wuchereria infections on the coast of Kenya

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Development of a specimen-sparing multichannel bead assay to detect antiparasite IgG4 for the diagnosis of Schistosoma and Wuchereria infections on the coast of Kenya

Adam S DuVall et al. Am J Trop Med Hyg. 2014 Apr.

Abstract

To better delineate the impact of parasitic coinfection in coastal Kenya, we developed a novel specimen-sparing bead assay using multiplex flow immunoassay (MFI) technology to simultaneously measure serum or plasma immunoglobulin G4 (IgG4) against Brugia malayi antigen (BMA) and Schistosoma haematobium soluble worm antigen (SWAP). Properties of the bead assay were estimated by latent class analysis using data from S. haematobium egg counts/filarial rapid diagnostic cards (RDTs), parasite-specific enzyme-linked immunosorbent assays (ELISAs), and the multichannel IgG4 assay. For schistosomiasis, the bead assay had an estimated sensitivity of 81% and a specificity of 45%, and it was more sensitive than ELISA or urine egg counts for diagnosing infection. For filariasis, it had a sensitivity of 86% and a specificity of 39%, and it was more sensitive than ELISA or RDT. Measuring antibody by MFI is feasible and may provide more accurate epidemiological information than current parasitological tests, especially in the setting of low-intensity infections.

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Figures

Figure 1.
Figure 1.
Relationship between SWAP IgG4 multiplex assay fluorescence and SWAP IgG4 ELISA optical density.
Figure 2.
Figure 2.
Relationship between BMA IgG4 multiplex assay fluorescence and BMA IgG4 ELISA optical density.
Figure 3.
Figure 3.
Two-class model for estimation of the diagnostic performance of the novel antiparasite IgG4 multiplex assays compared with standard parasitology and classical ELISA technique.

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