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. 2013 Nov;89(5):916-8.
doi: 10.4269/ajtmh.13-0245. Epub 2013 Sep 9.

Semi-quantitative scoring of an immunochromatographic test for circulating filarial antigen

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Semi-quantitative scoring of an immunochromatographic test for circulating filarial antigen

Cédric B Chesnais et al. Am J Trop Med Hyg. 2013 Nov.

Abstract

Abstract. The value of a semi-quantitative scoring of the filarial antigen test (Binax Now Filariasis card test, ICT) results was evaluated during a field survey in the Republic of Congo. One hundred and thirty-four (134) of 774 tests (17.3%) were clearly positive and were scored 1, 2, or 3; and 11 (1.4%) had questionable results. Wuchereria bancrofti microfilariae (mf) were detected in 41 of those 133 individuals with an ICT test score ≥ 1 who also had a night blood smear; none of the 11 individuals with questionable ICT results harbored night mf. Cuzick's test showed a significant trend for higher microfilarial densities in groups with higher ICT scores (P < 0.001). The ICT scores were also significantly correlated with blood mf counts. Because filarial antigen levels provide an indication of adult worm infection intensity, our results suggest that semi-quantitative reading of the ICT may be useful for grading the intensity of filarial infections in individuals and populations.

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Figures

Figure 1.
Figure 1.
Frequency distributions of the immunochromatographic card test (ICT) scores in microfilaremic (striped boxes) and amicrofilaremic (white boxes) subjects with positive ICT test results.
Figure 2.
Figure 2.
Distribution of Wuchereria bancrofti microfilarial densities by immunochromatographic card test (ICT) score for microfilaremic subjects (left panel) and for all ICT positive subjects (right panel). Horizontal lines represent arithmetic means and 95% confidence intervals.

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