This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features!
Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

NIH NLM Logo
Log in
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2011 Jul:107 Suppl:S42-8.
doi: 10.1016/j.jip.201105004.

Large-scale production and purification of VLP-based vaccines

Affiliations
Review

Large-scale production and purification of VLP-based vaccines

Tiago Vicente et al. J Invertebr Pathol. 2011 Jul.

Abstract

Virus-like particles (VLPs) hold tremendous potential as vaccine candidates. These innovative biopharmaceuticals present the remarkable advantages of closely mimicking the three-dimensional nature of an actual virus while lacking the virus genome packaged inside its capsid. As a result, an equally efficient but safer prophylaxis is anticipated as compared to inactivated or live attenuated viral vaccines. With the advent of successful cases of approved VLP-based vaccines, pharmaceutical companies are indeed redirecting their resources to the development of such products. This paper reviews the current choices and trends of large-scale production and purification of VLP-based vaccines generated through the baculovirus expression vector system using insect cells.

PubMed Disclaimer

Figures

None
Graphical abstract
Fig. 1
Fig. 1
Downstream process strategy for rotavirus VLP purification (Peixoto et al., 2007); note the inclusion of a lysis step to help release the VLPs from the cell host. Reproduced from Peixoto et al. (2007).

References

    1. CBER guidelines, 2007. US Food and Drug Administration, Center for Biologics Evaluation and Research.
    1. Almanza H., Cubillos C., Angulo I., Mateos F., Caston J.R., van der Poel W.H., Vinje J., Barcena J., Mena I. Self-assembly of the recombinant capsid protein of a swine norovirus into virus-like particles and evaluation of monoclonal antibodies cross-reactive with a human strain from genogroup II. J. Clin. Microbiol. 2008;46:3971–3979. - PMC - PubMed
    1. Bachmann A.S., Corpuz G., Hareld W.P., Wang G., Coller B.A. A simple method for the rapid purification of copia virus-like particles from Drosophila Schneider 2 cells. J. Virol. Methods. 2004;115:159–165. - PubMed
    1. Bernal V., Carinhas N., Yokomizo A.Y., Carrondo M.J., Alves P.M. Cell density effect in the baculovirus–insect cells system: a quantitative analysis of energetic metabolism. Biotechnol. Bioeng. 2009;104:162–180. - PubMed
    1. Betenbaugh M., Yu M., Kuehl K., White J., Pennock D., Spik K., Schmaljohn C. Nucleocapsid- and virus-like particles assemble in cells infected with recombinant baculoviruses or vaccinia viruses expressing the M and the S segments of Hantaan virus. Virus Res. 1995;38:111–124. - PubMed

Publication types

Substances

Full text links
Elsevier Science full text link Elsevier Science Free PMC article
Cite

AltStyle によって変換されたページ (->オリジナル) /