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. 2010 May;82(5):889-98.
doi: 10.4269/ajtmh.2010.09-0711.

Serologic and molecular studies of Leptospira and leptospirosis among rats in the Philippines

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Serologic and molecular studies of Leptospira and leptospirosis among rats in the Philippines

Sharon Y A M Villanueva et al. Am J Trop Med Hyg. 2010 May.

Abstract

Rats are known to be the most important reservoirs and transmission sources of leptospirosis. However, the status of leptospirosis in the Philippines regarding reservoirs and transmission remains unknown. A survey was conducted in Metro Manila and Laguna that analyzed samples obtained from 106 rats. Using the microscopic agglutination test, we found that 92% of rat serum samples were positive for anti-Leptospira antibodies; the most common infecting serovars were Manilae, Hebdomadis, and Losbanos. On the basis of pulsed-field gel electrophoresis and gyrase B gene sequence analyses, four groups of rat kidney isolates were found: L. interrogans serovar Manilae, serovar Losbanos, and serogroup Grippotyphosa, and L. borgpetersenii serogroup Javanica. Most isolates were lethal after experimental infection of golden Syrian hamsters. Results showed that these four Leptospira serovars and serogroups are circulating among rats, and that these animals may be one of the possible transmission sources of leptospirosis in the Philippines.

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Figures

Figure 1.
Figure 1.
Representative pulse-filed gel electrophoresis band patterns of the rat kidney isolates and reference strains, the Philippines. Group A = rat kidney isolates (represented by K59, K60, and K64) having identical band patterns as Leptospira interrogans serovar Manilae strain LT 398. Group B = rat kidney isolates (represented by K37, E38, and E41) having the same band patterns as L. interrogans serovar Losbanos strain LT 101-69. Group C = rat kidney isolates (represented by K5, E7, and K16) with band patterns of smaller DNA similar to serovar Manilae; isolates were classified as L. interrogans serogroup Grippotyphosa. Group D = rat kidney isolates (represented by K6, K35, and E63) with band patterns identical to several strains belonging to L. borgpetersenii serogroup Javanica. M = lambda marker; sv = serovar; VB 46 = Veldrat Batavia 46.
Figure 2.
Figure 2.
Phylogenetic tree based on gyrase B gene sequences. Sequence accession numbers are indicated in parenthesis. Underlined strains represent the isolates obtained in this study. Bar = sequence divergence of 5%. sg = serogroup.

References

    1. Famatiga EG, Topacio TM, Suva MH, Oliveros FM. Studies on leptospirosis in animals and man in the Philippines V. Serological survey of leptospirosis among occupationally exposed Filipinos. Southeast Asian J Trop Med Public Health. 1972;3:482–487.
    1. World Health Organization . Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control. Geneva: World Health Organization and International Leptospirosis Society; 2003.
    1. Faine S, Adler B, Bolin C, Perolat P. Leptospira and Leptospirosis. Melbourne, Australia: MediSci; 1999.
    1. Wisseman CL, Jr, Traub R, Gochenour WS, Jr, Smadel JE, Lancaster WE. Leptospirosis of man and animals in urban, rural and jungle areas of southeast Asia. Am J Trop Med Hyg. 1955;4:29–40. - PubMed
    1. Levett PN. Leptospirosis: a forgotten zoonosis? Clin Appl Immunol Rev. 2004;4:435–448.

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