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Comparative Study
. 2003 Apr;41(4):1753-5.
doi: 10.1128/JCM.41.4.1753-1755.2003.

Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples

Affiliations
Comparative Study

Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples

Andrea Bracca et al. J Clin Microbiol. 2003 Apr.

Abstract

We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.

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Figures

FIG. 1.
FIG. 1.
Sensitivity of seminested PCR assay. (A) Schematic representation of primers location at the H. capsulatum H antigen (Ag) gene. Primer sequences are described in the text. (B) Amplification products of both the first reaction and seminested PCR, analyzed by ethidium bromide-stained agarose gel electrophoresis. The amounts of DNA (expressed as genome equivalents) loaded in the first reaction were 5 ×ばつ 106 (lane 1), 2.5 ×ばつ 105 (lane 2), 5 ×ばつ 104 (lane 3), 5 ×ばつ 103 (lane 4), 5 ×ばつ 102 (lane 5), 5 ×ばつ 101 (lane 6), and 5 (lane 7).

References

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