Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples
- PMID: 12682178
- PMCID: PMC153865
- DOI: 10.1128/JCM.41.4.1753-1755.2003
Molecular detection of Histoplasma capsulatum var. capsulatum in human clinical samples
Abstract
We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.
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References
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- Bullok, W. E. 1995. Histoplasma capsulatum, p. 2340-2353. In G. L. Mandell, J. Bennet, and R. Dolin (ed.), Principles and practice of infectious diseases. Churchill Livingstone, New York, N.Y.
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- Fotjasek, M. F., M. B. Kleiman, P. Connolly-Stringfield, R. Blair, and J. Wheat. 1994. The Histoplasma capsulatum antigen assay in disseminated histoplasmosis in children. Pediatr. Infect. Dis. J. 13:801-805. - PubMed
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