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. 2003 Mar;41(3):1295-8.
doi: 10.1128/JCM.41.3.1295-1298.2003.

Identification of Histoplasma capsulatum from culture extracts by real-time PCR

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Identification of Histoplasma capsulatum from culture extracts by real-time PCR

Jose Martagon-Villamil et al. J Clin Microbiol. 2003 Mar.

Abstract

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.

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Figures

FIG. 1.
FIG. 1.
PCR analysis results. The positive PCR results include (i) cultured isolates of H. capsulatum (×ばつ), which include the ATCC positive control strain and sample 81, and (ii) clinical specimens that were culture positive for H. capsulatum (black squares), which include a formalin-fixed bone marrow clot sample, a blood sample, a bronchoalveolar lavage sample, and an open-lung biopsy sample. The negative PCR results (—) depicted include (i) the negative control (TE buffer), (ii) a representative non-H. capsulatum culture isolate (C. albicans; sample 83), and (iii) the B5 fixed bone marrow biopsy sample (tissue 73B).

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