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Case Reports
. 2002 Jul;8(7):657-61.
doi: 10.3201/eid0807.010277.

First human isolate of Hantavirus (Andes virus) in the Americas

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Case Reports

First human isolate of Hantavirus (Andes virus) in the Americas

Hector Galeno et al. Emerg Infect Dis. 2002 Jul.

Abstract

We isolated Andes virus (formal name: Andes virus [ANDV], a species in the genus Hantavirus), from serum of an asymptomatic 10-year-old Chilean boy who died 6 days later of hantavirus pulmonary syndrome (HPS). The serum was obtained 12 days after his grandmother died from HPS and 2 days before he became febrile. No hantavirus immunoglobulin (Ig) G or IgM antibodies were detected in the serum sample. After three blind passages, ANDV antigens were detected in Vero E6 cells by immunofluorescence assay and enzyme-linked immunosorbent assay, and ANDV RNA was detected by reverse transcription-polymerase chain reaction. A fragment of the virus genome showed 96.2% nucleotide identity with that of prototype ANDV. To our knowledge, this is the first isolation of any agent of hemorrhagic fever with HPS from a human and the first such isolation of hantavirus before symptoms of that syndrome or HPS began.

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Figures

Figure 1
Figure 1
Immunofluorescence assay (IFA) of Vero E6 cells infected with Chilean hantavirus CHI-7913 isolate. A, IFA with seropositive human sera from a Chilean HPS patient; arrow shows infected Vero E6 cells expressing hantavirus antigens. B, IFA with seronegative human sera from uninfected control; arrow shows the negative IFA of Vero E6 cells infected with the CHI-7913 isolate.
Figure 2
Figure 2
Maximum-parsimony tree analysis comparing S nucleotide sequence of CHI-7913 virus with homologous sequences of previously characterized hantaviruses. Nucleotide sequences examined correspond to positions 22–359 of antigenome-sense sequence of nucleoprotein (N) gene. Sequences were analyzed by the maximun parsimony method with the Clustal W and PHYLIP packages . The minimal length trees shown were supported as the majority rule consensus tree in 500 replicates. The bootstrap replicates supporting each node are indicated. References and GenBank accession numbers for the sequences used in S segment comparisons are BCC L39949; BAY L36929; NY strain RI-1 , U09488; SN strain cc107 , L33683; SN strain nmh10 , L25784; PH strain PH-1 , and M34011; Puumala strains Sotkamo , X61035; Seoul (SEO) strain sr-11 , and M34881; Hantaan (HTN) strain 76-118 , M14626; Andes strain AH-1 , AF004660; ESQ H-1/96 (14), AF005948; CH H-1/96 , AF 005947; AND Nort (strain unpublished) AF325966; and Andes strain 23 (AF291702).

References

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