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. 2001 Dec;45(12):3635-9.
doi: 10.1128/AAC.45.12.3635-3639.2001.

Multidrug resistant Mycobacterium leprae from patients with leprosy

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Multidrug resistant Mycobacterium leprae from patients with leprosy

S Maeda et al. Antimicrob Agents Chemother. 2001 Dec.

Abstract

Sequences of the folP1, rpoB, and gyrA genes were analyzed for 88 isolates of Mycobacterium leprae from leprosy patients in Japan, Haiti, Indonesia, Pakistan, and the Philippines. Thirteen isolates (14.8%) showed representative mutations in more than two genes, suggesting the emergence of multidrug-resistant M. leprae.

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Figures

FIG. 1
FIG. 1
Nucleotide sequence of the folP1 gene from Thai 53 and clinical isolates of M. leprae. A DNA fragment of folP1 (388 bp) was amplified by PCR and sequenced. In 19 isolates of M. leprae, mutations at positions 53 and 55, which were found to be associated with dapsone resistance, were detected (the numbering system of reference was used). The numbers of cases which had a mutation are shown. One isolate was overlapping in this figure because it had mutations at positions 157 and 164.
FIG. 2
FIG. 2
DNA sequence of the rpoB gene from clinical isolates of M. leprae. The rpoB sequence from positions 1243 to 1547 was amplified and analyzed. Twenty-six isolates had a mutation at position 441 (516), 451 (526), 456 (531), or 458 (533) (E. coli numbering in parentheses [16]). One isolate had mutations at positions 441 (516) and 458 (533).
FIG. 3
FIG. 3
Structure and nucleotide sequence of the gyrA gene from clinical isolates of M. leprae. To analyze the sequence, the gyrA sequence from positions 1 to 390 was amplified by PCR. Mutations at positions 89 and 91 were found in ofloxacin-resistant M. leprae. The numbering system of reference was used.

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