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. 2000 Jun;38(6):2403-6.
doi: 10.1128/JCM.38.6.2403-2406.2000.

Epidemiologic typing of Salmonella enterica serotype enteritidis in a Canada-wide outbreak of gastroenteritis due to contaminated cheese

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Epidemiologic typing of Salmonella enterica serotype enteritidis in a Canada-wide outbreak of gastroenteritis due to contaminated cheese

R Ahmed et al. J Clin Microbiol. 2000 Jun.

Abstract

A major Canada-wide outbreak of gastroenteritis due to Salmonella enterica serotype Enteritidis phage type (PT) 8 occurred in 1998, and this was traced to contaminated cheese in a commercial lunch pack product. Phage typing and pulsed-field gel electrophoresis linked the clinical and cheese isolates of serotype Enteritidis but failed to differentiate outbreak from nonoutbreak PT 8 strains. Further differentiation was made by biotyping based on melibiose fermentation.

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Figures

FIG. 1
FIG. 1
Characterization of selected S. enterica serotype Enteritidis strains by PFGE following digestion with the restriction endonuclease XbaI. Lanes 2 to 4, 8, and 9, outbreak isolates (all PT 8, melibiose negative); lanes 5 and 7, cheese isolates (PT 8, melibiose negative); lanes 10, 12, 13, 14, and 15, nonoutbreak control isolates (PT4, PT13a, PT8, PT8, and PT untypeable, respectively; all melibiose positive). Escherichia coli CDC strain G5244 was used as a molecular size marker (lanes 1, 6, and 11). Molecular sizes are indicated in kilobases.

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