PCR法を用いた米加工品の安全性未審査遺伝子組換え米の検知法

タイトル別名

• A conventional PCR method to detect recombinant DNA from genetically modified rice lines not approved for use in processed foods
• PCRホウ オ モチイタ コメ カコウヒン ノ アンゼンセイ ミシンサ イデンシ クミカエ マイ ノ ケンチホウ

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• CiNii Books

Detection methods using a conventional polymerase chain reaction (PCR) were developed to detect two lines of genetically modified (GM) rice that have not been approved as safe for use in foods in Japan. Two GM rice lines were Bacillus thuringiensis (Bt) rice lines (Bt63 and NNBt) in which cry genes derived from Bt conjugated with a suitable plant expression promoter and terminator are transformed, expressing the Bt toxin protein confers a tolerance against insects. We designed primer pairs to detect the two lines of GM rice based on the common cry gene sequence. We also designed the primer pairs to specifically detect Bt63 and NNBt rice, respectively, on the sequences we had already clarified. The proposed method was applied to the detection of the two Bt rice lines in rice vermicelli and glutinous rice samples. We showed that the present conventional method was a reasonable assay for monitoring two Bt rice lines in processed rice products.